A novel lipid droplets/lysosomes-targeting colorimetric and ratiometric fluorescent probe for Cu2+ and its application.
Spectrochim Acta A Mol Biomol Spectrosc
; 291: 122333, 2023 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-36621028
ABSTRACT
A novel multifunctional fluorescent probe LL2 was prepared via a one-step condensation reaction between 3-formyl-N-butylcarbazole and 2-Hydroxy-1-naphthylhydrazone. LL2 can work as a colorimetric probe for Cu2+, and can also selectively recognize Cu2+ via ratiometric fluorescence signal. After the addition of Cu2+, the probe LL2 responded rapidly within 5 s and reached stability within 30 s. In natural light, when Cu2+ were added to the solution, the color of probe LL2 changed from yellowish to colorless, while there was a discernible fluorescence changed from green to blue under a 365 nm UV lamp. The ratiometric fluorescence intensity (F449/F510) showed a good linear relationship (R2 = 0.9902) with Cu2+ concentration in the concentration range of 0-5 µmol/L, and the minimum detection limit was 1.96 µM. Cell imaging experiments showed that LL2 could capture fluorescence signals in the green and blue channels of HepG2 cells through fluorescence confocal microscope, and successfully recognize exogenous Cu2+ in HepG2 cells. In addition, fluorescence co-localization experiments showed that LL2 could target both lipid droplets and lysosomes. Meanwhile, LL2 could be applied to filter paper strip assay and detection of Cu2+ in actual water samples. These results indicated that probe LL2 has a good capability for monitoring Cu2+ in environment and living cells.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Colorimetría
/
Colorantes Fluorescentes
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2023
Tipo del documento:
Article