Detection of diatoms in blood by a combination of membrane filtering and chemical digestion.
Forensic Sci Int
; 34(3): 175-82, 1987 Jul.
Article
en En
| MEDLINE
| ID: mdl-3666621
ABSTRACT
An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 microns in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sangre
/
Ahogamiento
/
Eucariontes
/
Medicina Legal
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Child
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Forensic Sci Int
Año:
1987
Tipo del documento:
Article
País de afiliación:
Japón