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Genomic features and heat resistance profiles of Escherichia coli isolated from Brazilian beef.
Machado, Maxsueli Aparecida Moura; Castro, Vinicius Silva; da Cunha Neto, Adelino; Carvalho, Ricardo César Tavares; Stanford, Kim; Conte-Junior, Carlos Adam; Figueiredo, Eduardo Eustáquio de Souza.
Afiliación
  • Machado MAM; Postgraduate Program in Food, Nutrition and Metabolism (PPGNAM), Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso 78060-900, Brazil.
  • Castro VS; Postgraduate program in Food Science (PPGCAL), Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, Brazil.
  • da Cunha Neto A; Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitaria, Rio de Janeiro, RJ 21941-598, Brazil.
  • Carvalho RCT; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universitaria, Rio de Janeiro, RJ 21941-901, Brazil.
  • Stanford K; University of Lethbridge, 4401 University Drive Lethbridge, Lethbridge Campus, Alberta T1K 3M4, Canada.
  • Conte-Junior CA; Postgraduate program in Animal Science (PPGCA), Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso 78060-900, Brazil.
  • Figueiredo EES; Postgraduate Program in Food, Nutrition and Metabolism (PPGNAM), Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso 78060-900, Brazil.
J Appl Microbiol ; 134(1)2023 Jan 23.
Article en En | MEDLINE | ID: mdl-36724247
AIMS: Characterize Escherichia coli and E. coli -producing (STEC) isolates from Brazilian beef to determine heat resistance and the presence of the transmissible locus of stress tolerance (tLST). METHODS AND RESULTS: Twenty-two STEC previously isolated from beef and characterized as STEC by PCR were subjected to different heat survival challenges (60°C and 71°C). Furthermore, the three tLST-positive isolates and one tLST-negative isolate by PCR were selected for WGS analysis. Phenotypic results indicated that 3/22 (13.64%) were heat resistant, 12/22 (54.54%) were moderately resistant, and 7/22 (31.82%) were sensitive to heat treatments. WGS analyses showed that three isolates with heat resistance showed tLST with up to 80% and 42% of similarity by BLAST analysis, with the major tLST genes being responsible for the homeostasis module. However, WGS showed the absence of stx genes associated with tLST-positive isolates, albeit with virulence and resistance genes found in extraintestinal pathogenic E. coli (ExPEC). CONCLUSION: Our findings demonstrate the presence of heat-resistant E. coli as well as confirm some tLST genes in E. coli isolated from Brazilian beef.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil