Genetic ablation: targeted expression of a toxin gene causes microphthalmia in transgenic mice.
Science
; 238(4833): 1563-5, 1987 Dec 11.
Article
en En
| MEDLINE
| ID: mdl-3685993
ABSTRACT
Lineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene encoding a cytotoxic gene product, then genetic ablation of a specific cell lineage can be achieved. We have generated six transgenic mice by introducing into fertilized eggs the mouse gamma 2-crystallin promoter fused to the coding region of the diphtheria toxin A-chain gene. Three of these mice and all the transgenic offspring analyzed were microphthalmic. The lenses of these mice displayed considerable heterogeneity some were almost normal morphologically but reduced in size, whereas others were grossly aberrant and deficient in nuclear fiber cells. These studies indicate that programmed ablation of specific cell types can be stably transmitted through the germ line.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Microftalmía
/
Cristalinas
/
Toxina Diftérica
/
Genes
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
1987
Tipo del documento:
Article
País de afiliación:
Canadá