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Berberine Enhances Intestinal Mucosal Barrier Function by Promoting Vitamin D Receptor Activity.
Huang, Yong-Quan; Liu, Jia-Lin; Chen, Geng-Xin; Shen, Dan-Ting; Zhu, Wang; Chen, Xin-Lin; Liu, Feng-Bin; Hou, Qiu-Ke.
Afiliación
  • Huang YQ; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Liu JL; Department of Orthopedics, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
  • Chen GX; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Shen DT; Department of Orthopedics, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
  • Zhu W; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Chen XL; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Liu FB; Department of Preventive Medicine and Health Statistics, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
  • Hou QK; Department of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
Chin J Integr Med ; 30(2): 143-151, 2024 Feb.
Article en En | MEDLINE | ID: mdl-37046128
OBJECTIVE: To evaluate if berberine can act on vitamin D receptors (VDR) and thereby regulate the expression of tight junction proteins (TJPs) in irritable bowel syndrame-diarrhea-predominant (IBS-D) rats. METHODS: The newborn rats were induced into IBS-D rat model via neonatal maternal separation combined with acetic acid chemical stimulation. After modeling, the model was evaluated and rats were divided into the control group and berberine treatment groups (0.85, 1.7 and 3.4 mg/kg, once a day for 2 weeks). The distal colon was obtained and colonic epithelial cells (CECs) were isolated and cultured after IBS-D model evaluation. The vitamin D receptor response element (VDRE) reporter gene was determined in the CECs of IBS-D rats to analyze the effect of berberine on the VDRE promoter. VDR overexpression or silencing technology was used to analyze whether VDR plays a role in promoting intestinal barrier repair, and to determine which region of VDR plays a role in berberine-regulated intestinal TJPs. RESULTS: The IBS-D rat model was successfully constructed and the symptoms were improved by berberine in a dose-dependent manner (P<0.05). The activity of VDRE promoter was also effectively promoted by berberine (P<0.05). Berberine increased the expression of TJPs in IBS-D CECs (P<0.05). VDR expression was significantly increased after transfection of different domains of VDR when compared to normal control and basic plasmid groups (all P<0.05). RT-qPCR and Western blot results showed that compared with the blank group, expressions of occludin and zonula occludens-1 were significantly higher in VDR containing groups (all P<0.05). Berberine plus pCMV-Myc-VDR-N group exerted the highest expression levels of occludin and zonula occludens-1 (P<0.05). CONCLUSION: Berberine enhances intestinal mucosal barrier function of IBS-D rats by promoting VDR activity, and the main site of action is the N-terminal region of VDR.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Berberina / Síndrome del Colon Irritable Límite: Animals Idioma: En Revista: Chin J Integr Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Berberina / Síndrome del Colon Irritable Límite: Animals Idioma: En Revista: Chin J Integr Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2024 Tipo del documento: Article País de afiliación: China