First gene-edited calf with reduced susceptibility to a major viral pathogen.
PNAS Nexus
; 2(5): pgad125, 2023 May.
Article
en En
| MEDLINE
| ID: mdl-37181049
ABSTRACT
Bovine viral diarrhea virus (BVDV) is one of the most important viruses affecting the health and well-being of bovine species throughout the world. Here, we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by reduced clinical signs and the lack of viral infection in white blood cells. The edited calf has no off-target edits and appears normal and healthy at 20 months of age without obvious adverse effects from the on-target edit. This precision bred, proof-of-concept animal provides the first evidence that intentional genome alterations in the CD46 gene may reduce the burden of BVDV-associated diseases in cattle and is consistent with our stepwise, in vitro and ex vivo experiments with cell lines and matched fetal clones.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
PNAS Nexus
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos