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Advances in proteomics methods for the analysis of exhaled breath condensate.
Yoo, Edwin J; Kim, Julie S; Stransky, Stephanie; Spivack, Simon; Sidoli, Simone.
Afiliación
  • Yoo EJ; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA.
  • Kim JS; Department of Medicine, Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Stransky S; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA.
  • Spivack S; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA.
  • Sidoli S; Department of Medicine, Department of Epidemiology & Population Health, Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, USA.
Mass Spectrom Rev ; 43(4): 713-722, 2024.
Article en En | MEDLINE | ID: mdl-38149478
ABSTRACT
The analysis of exhaled breath condensate (EBC) demonstrates a promising avenue of minimally invasive biopsies for diagnostics. EBC is obtained by cooling exhaled air and collecting the condensation to be utilized for downstream analysis using various analytical methods. The aqueous phase of breath contains a large variety of miscible small compounds including polar electrolytes, amino acids, cytokines, chemokines, peptides, small proteins, metabolites, nucleic acids, and lipids/eicosanoids-however, these analytes are typically present at minuscule levels in EBC, posing a considerable technical challenge. Along with recent improvements in devices for breath collection, the sensitivity and resolution of liquid chromatography coupled to online mass spectrometry-based proteomics has attained subfemtomole sensitivity, vastly enhancing the quality of EBC sample analysis. As a result, proteomics analysis of EBC has been expanding the field of breath biomarker research. We present an au courant overview of the achievements in proteomics of EBC, the advancement of EBC collection devices, and the current and future applications for EBC biomarker analysis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría de Masas / Pruebas Respiratorias / Biomarcadores / Proteómica / Espiración Límite: Humans Idioma: En Revista: Mass Spectrom Rev Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría de Masas / Pruebas Respiratorias / Biomarcadores / Proteómica / Espiración Límite: Humans Idioma: En Revista: Mass Spectrom Rev Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos