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Deregulated expression of autophagy genes; PIK3C3 and RAB7A in COVID-19 patients.
Tahoun, Mona; Sadaka, Ahmed S.
Afiliación
  • Tahoun M; Clinical and Chemical Pathology Department, Faculty of Medicine, Alexandria University, Egypt. Electronic address: mona.tahoun@alexmed.edu.eg.
  • Sadaka AS; Chest Diseases Department, Faculty of Medicine, Alexandria University, Egypt.
Hum Immunol ; 85(3): 110801, 2024 May.
Article en En | MEDLINE | ID: mdl-38609772
ABSTRACT

BACKGROUND:

The role of autophagy in coronaviruses infection and replication has a lot of debate. Autophagy involves the catalytic breakdown of intracellular components to be subsequently recycled by the lysosome. The aim of the study was to evaluate autophagy genes; PIK3C3 and RAB7A expressions in COVID-19 patients, and identify if PIK3C3 and RAB7A can be used as markers for monitoring COVID-19 patients.

METHODS:

A case-control study was carried out on 50 patients and 50 healthy controls. Genes expression was performed using quantitative real-time polymerase chain reaction.

RESULTS:

Compared to controls, PIK3C3 and RAB7A gene expression levels were significantly lower in patients (p < 0.001) with approximately with 9.4 and 2.3 decreased fold in PIK3C3 and RAB7A respectively. The ROC curve of PIK3C3 and RAB7A expressions showed sensitivity of 84 % and 74 % and specificity of 98 % and 78 % respectively. There was a positive correlation between PIK3C3 expression and WBCs, absolute neutrophil count, interleukin-6, D-dimer, and ALT among patients and between RAB7A expression and WBCs, CRP, IL-6, D-dimer and ALT in patients.

CONCLUSIONS:

The study showed reduction of PIK3C3 and RAB7A expressions in COVID-19 patients. However, further studies are recommended to clarify their roles in the disease pathogenies as autophagy genes.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Proteínas de Unión al GTP rab / Fosfatidilinositol 3-Quinasas Clase III / COVID-19 / Proteínas de Unión a GTP rab7 Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Proteínas de Unión al GTP rab / Fosfatidilinositol 3-Quinasas Clase III / COVID-19 / Proteínas de Unión a GTP rab7 Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Hum Immunol Año: 2024 Tipo del documento: Article