Competition between Self-Assembly and Phase Separation Governs High-Temperature Condensation of a DNA Liquid.
Phys Rev Lett
; 132(20): 208401, 2024 May 17.
Article
en En
| MEDLINE
| ID: mdl-38829088
ABSTRACT
In many biopolymer solutions, attractive interactions that stabilize finite-sized clusters at low concentrations also promote phase separation at high concentrations. Here we study a model biopolymer system that exhibits the opposite behavior, whereby self-assembly of DNA oligonucleotides into finite-sized, stoichiometric clusters tends to inhibit phase separation. We first use microfluidics-based experiments to map a novel phase transition in which the oligonucleotides condense as the temperature increases at high concentrations of divalent cations. We then show that a theoretical model of competition between self-assembly and phase separation quantitatively predicts changes in experimental phase diagrams arising from DNA sequence perturbations. Our results point to a general mechanism by which self-assembly shapes phase boundaries in complex biopolymer solutions.
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MEDLINE
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Idioma:
En
Revista:
Phys Rev Lett
Año:
2024
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Article
País de afiliación:
Estados Unidos