Protocol for profiling virus-to-host RNA-RNA interactions in infected cells by RIC-seq.
STAR Protoc
; 5(3): 103149, 2024 Sep 20.
Article
en En
| MEDLINE
| ID: mdl-38907997
ABSTRACT
Virus-to-host RNA-RNA interactions directly regulate host mRNA stability and viral replication. However, globally profiling virus-to-host in situ RNA-RNA interactions remains challenging. Here, we present an RNA in situ conformation sequencing (RIC-seq)-based protocol for mapping high-confidence virus-to-host in situ RNA-RNA interactions in infected cells. We detail steps for formaldehyde crosslinking, pCp-biotin labeling, in situ proximity ligation, chimeric RNA enrichment, strand-specific library construction, and data analysis. This protocol allows unbiased identification of virus-to-host RNA-RNA interactions for various RNA viruses and is potentially applicable to DNA virus-derived transcripts. For complete details on the use and execution of this protocol, please refer to Zhao et al.1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Viral
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article