TGFß1 Regulates Cellular Composition of In Vitro Cardiac Perivascular Niche Based on Cardiospheres.
Bull Exp Biol Med
; 177(1): 115-123, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38963596
ABSTRACT
The cardiac perivascular niche is a cellular microenvironment of a blood vessel. The principles of niche regulation are still poorly understood. We studied the effect of TGFß1 on cells forming the cardiac perivascular niche using 3D cell culture (cardiospheres). Cardiospheres contained progenitor (c-Kit), endothelial (CD31), and mural (αSMA) cells, basement membrane proteins (laminin) and extracellular matrix proteins (collagen I, fibronectin). TGFß1 treatment decreased the length of CD31+ microvasculature, VE cadherin protein level, and proportion of NG2+ cells, and increased proportion of αSMA+ cells and transgelin/SM22α protein level. We supposed that this effect is related to the stabilizing function of TGFß1 on vascular cells decreased endothelial cell proliferation, as shown for HUVEC, and activation of mural cell differentiation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Proliferación Celular
/
Factor de Crecimiento Transformador beta1
Idioma:
En
Revista:
Bull Exp Biol Med
Año:
2024
Tipo del documento:
Article
País de afiliación:
Rusia