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Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching.
Xu, Chuanyun; Li, Zhuoran; Lyu, Cheng; Hu, Yixin; McLaughlin, Colleen N; Wong, Kenneth Kin Lam; Xie, Qijing; Luginbuhl, David J; Li, Hongjie; Udeshi, Namrata D; Svinkina, Tanya; Mani, D R; Han, Shuo; Li, Tongchao; Li, Yang; Guajardo, Ricardo; Ting, Alice Y; Carr, Steven A; Li, Jiefu; Luo, Liqun.
Afiliación
  • Xu C; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
  • Li Z; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
  • Lyu C; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Hu Y; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
  • McLaughlin CN; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Wong KKL; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Xie Q; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Graduate Program, Stanford University, Stanford, CA 94305, USA.
  • Luginbuhl DJ; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Li H; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Udeshi ND; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Svinkina T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Mani DR; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Han S; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
  • Li T; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Li Y; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
  • Guajardo R; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Ting AY; Departments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
  • Carr SA; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Li J; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. Electronic address: lij6@janelia.hhmi.org
  • Luo L; Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu.
Cell ; 187(18): 5081-5101.e19, 2024 Sep 05.
Article en En | MEDLINE | ID: mdl-38996528
ABSTRACT
In developing brains, axons exhibit remarkable precision in selecting synaptic partners among many non-partner cells. Evolutionarily conserved teneurins are transmembrane proteins that instruct synaptic partner matching. However, how intracellular signaling pathways execute teneurins' functions is unclear. Here, we use in situ proximity labeling to obtain the intracellular interactome of a teneurin (Ten-m) in the Drosophila brain. Genetic interaction studies using quantitative partner matching assays in both olfactory receptor neurons (ORNs) and projection neurons (PNs) reveal a common pathway Ten-m binds to and negatively regulates a RhoGAP, thus activating the Rac1 small GTPases to promote synaptic partner matching. Developmental analyses with single-axon resolution identify the cellular mechanism of synaptic partner matching Ten-m signaling promotes local F-actin levels and stabilizes ORN axon branches that contact partner PN dendrites. Combining spatial proteomics and high-resolution phenotypic analyses, this study advanced our understanding of both cellular and molecular mechanisms of synaptic partner matching.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Axones / Sinapsis / Transducción de Señal / Neuronas Receptoras Olfatorias / Proteínas de Drosophila / Drosophila melanogaster / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: Cell Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Axones / Sinapsis / Transducción de Señal / Neuronas Receptoras Olfatorias / Proteínas de Drosophila / Drosophila melanogaster / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: Cell Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos