Development of nanobodies against the coat protein of maize chlorotic mottle virus.
FEBS Open Bio
; 14(10): 1746-1757, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39168939
ABSTRACT
Maize lethal necrosis (MLN) is a maize disease caused by the maize chlorotic mottle virus (MCMV), a potyvirus which causes yield losses of 30-100%. The present study aimed to isolate nanobodies against the MCMV coat protein (CP) for the diagnosis of MLN. MCMV CP expressed in Escherichia coli was used for llama immunization. VHH (i.e. variable heavy domain of heavy chain) gene fragments were prepared from blood drawn from the immunized llama and used to generate a library in E. coli TG1 cells. MCMV specific nanobodies were selected by three rounds of phage display and panning against MCMV CP. The selected nanobodies were finally expressed in E. coli WK6 cells and purified. Eleven MCMV-specific nanobodies were identified and shown to detect MCMV in infected maize plants. Thus, our results show that nanobodies isolated from llama immunized with MCMV CP can distinguish infected and healthy maize plants, potentially enabling development of affordable MCMV detection protocols.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Zea mays
/
Proteínas de la Cápside
/
Anticuerpos de Dominio Único
Límite:
Animals
Idioma:
En
Revista:
FEBS Open Bio
Año:
2024
Tipo del documento:
Article
País de afiliación:
Tanzania