[Interaction of BamH1 restrictase with synthetic substrates containing complete or partial recognition sites of this enzyme]. / Vzaimodeistvie restriktazy BamHI s sinteticheskimi substratami, soderzhashchimi polnyi ili chastichnyi uchastki uznavaniia étogo fermenta.
Mol Biol (Mosk)
; 18(1): 169-75, 1984.
Article
en Ru
| MEDLINE
| ID: mdl-6323970
ABSTRACT
BamHI restriction patterns of the self-complementary oligodeoxyribonucleotides were investigated. Cleavage of the oligonucleotides, containing full-length recognition site GGATCC. shows a usual type of restriction pattern. The oligonucleotide d(5'-TCCAGATCTGGA) contains part of the recognition sequences 5'-GATC flanked with the half-size recognition sequences 5'-TCC from its 5'-side and 5'-GGA from its 3'-side. Cleavage of this substrate shows a restriction pattern which could be explained as being cleaved within the recognition sequence d(5'-GGA...TCC) formed by the ends of two substrate molecules. At the same time cleavage within the sequence d(5'-GATC) does not take place. These results support a symmetric binding model of a restriction nuclease with its recognition site via interaction with one half of the recognition sequence.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
Oligonucleótidos
/
Enzimas de Restricción del ADN
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
1984
Tipo del documento:
Article