Transfer of the scorpion toxin receptor to an insensitive potassium channel.
Neuron
; 13(4): 961-6, 1994 Oct.
Article
en En
| MEDLINE
| ID: mdl-7946339
ABSTRACT
Voltage-dependent potassium channels belong to a family of structurally related cation channels that underlie the electrical activity of excitable cells. Many potassium channels are blocked with high affinity by scorpion toxins, whereas others are completely insensitive. We transferred toxin sensitivity from the highly sensitive Kv1.3 (KV3) to the insensitive Kv2.1 (DRK1) potassium channel by transferring the stretch of amino acids between transmembrane domains 5/6. We provide evidence that this S5-S6 linker, which has been shown to comprise the pore-forming region, is probably the only part of the ion channel that directly interacts with bound toxin. Using site-directed mutagenesis, we identified specific residues in the S5-S6 linker that are responsible for the acquisition of toxin sensitivity by Kv2.1.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Canales de Potasio
/
Canales de Sodio
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neuron
Asunto de la revista:
NEUROLOGIA
Año:
1994
Tipo del documento:
Article