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Plasmid maintenance functions encoded on Dictyostelium discoideum nuclear plasmid Ddp1.
Hughes, J E; Kiyosawa, H; Welker, D L.
Afiliación
  • Hughes JE; Department of Biology, Utah State University, Logan 84322-5500.
Mol Cell Biol ; 14(9): 6117-24, 1994 Sep.
Article en En | MEDLINE | ID: mdl-8065344
All of the plasmid-carried genes expressed during vegetative growth are essential for long-term maintenance of plasmid Ddp1 in the nucleus of Dictyostelium discoideum. Deletion of Ddp1 genes expressed only during development had no detectable effect on plasmid maintenance. Deletion of vegetatively expressed genes, either singly or in pairs, resulted in (i) a rapid loss of plasmid from cells grown in the absence of selection for plasmid retention, (ii) variation in the proportion of monomer to multimer forms of the plasmid molecules, and/or (iii) abnormalities in plasmid copy number. At least two plasmid-encoded gene products influence patterns of expression of plasmid genes.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plásmidos / Dictyostelium Límite: Animals Idioma: En Revista: Mol Cell Biol Año: 1994 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plásmidos / Dictyostelium Límite: Animals Idioma: En Revista: Mol Cell Biol Año: 1994 Tipo del documento: Article