Genomic sequence sampling: a strategy for high resolution sequence-based physical mapping of complex genomes.
Nat Genet
; 7(1): 40-7, 1994 May.
Article
en En
| MEDLINE
| ID: mdl-8075638
ABSTRACT
We present a simple and efficient method for constructing high resolution physical maps of large regions of genomic DNA based upon sampled sequencing. The physical map is constructed by ordering high density cosmid contigs and determining a sequence fragment from each end of every clone. The resulting map, which contains 30-50% of the complete DNA sequence, allows the identification of many genes and makes possible PCR amplification of virtually any part of the genome. We apply this strategy to the automated analysis of the genome of the primitive eukaryote Giardia lamblia and evaluate its applicability to the physical mapping and DNA sequencing of the human genome.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Paseo de Cromosoma
/
Genoma
/
Análisis de Secuencia de ADN
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
1994
Tipo del documento:
Article