Crystal structure and evolution of a transfer RNA splicing enzyme.
Science
; 280(5361): 279-84, 1998 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-9535656
ABSTRACT
The splicing of transfer RNA precursors is similar in Eucarya and Archaea. In both kingdoms an endonuclease recognizes the splice sites and releases the intron, but the mechanism of splice site recognition is different in each kingdom. The crystal structure of the endonuclease from the archaeon Methanococcus jannaschii was determined to a resolution of 2.3 angstroms. The structure indicates that the cleavage reaction is similar to that of ribonuclease A and the arrangement of the active sites is conserved between the archaeal and eucaryal enzymes. These results suggest an evolutionary pathway for splice site recognition.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Conformación Proteica
/
Empalme del ARN
/
Methanococcus
/
Evolución Molecular
/
Endorribonucleasas
Idioma:
En
Revista:
Science
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos