Acutely increased aquaporin-4 exhibits more potent protective effects in the cortex against single and repeated isoflurane-induced neurotoxicity in the developing rat brain.

Damage to hippocampus, cerebellum, and cortex associated with cognitive functions due to anesthetic-induced toxicity early in life may cause cognitive decline later. Aquaporin 4 (AQP4), a key protein in waste clearance pathway of brain, is involved in synaptic plasticity and neurocognition. We investigated the effects of single and repeated isoflurane (Iso) anesthesia on AQP4 levels and brain damage. Postnatal-day (P)7 Wistar albino rats were randomly assigned to Iso or Control (C) groups. For single-exposure, pups were exposed to 1.5% Iso in 30% oxygenated-air for 3-h at P7 (Iso1). For repeated-exposure, pups were exposed to Iso for 3 days, 3-h each day, at 1-day intervals (P7 + 9 + 11) starting at P7 (Iso3). C1 and C3 groups received only 30% oxygenated-air. Based on HE-staining and immunoblotting (Bax/Bcl-2, cleaved-caspase3 and PARP1) analyses, Iso exposures caused a higher degree of apoptosis in hippocampus. Anesthesia increased 4-hydroxynonenal (4HNE), oxidative stress marker; the highest ROS accumulation was determined in cerebellum. Increased inflammation (TNF-α, NF-κB) was detected. Multiple Iso-exposures caused more significant damage than single exposure. Moreover, 4HNE and TNF-α contributed synergistically to Iso-induced neurotoxicity. After anesthesia, higher expression of AQP4 was detected in cortex than hippocampus and cerebellum. There was an inverse correlation between increased AQP4 levels and apoptosis/ROS/inflammation. Correlation analysis indicated that AQP4 had a more substantial protective profile against oxidative stress than apoptosis. Remarkably, acutely increased AQP4 against Iso exhibited a more potent neuroprotective effect in cortex, especially frontal cortex. These findings promote further research to understand better the mechanisms underlying anesthesia-induced toxicity in the developing brain.

Being a Physician in Coronavirus Disease 2019 Pandemic and Alternative Health Care Service: Telemedicine: Prospective Survey Study.

Introduction: In this study, we aimed to detect anxiety levels of the physicians during the Coronavirus Disease 2019 (COVID-19) pandemic and to assess the knowledge, perspective, and willingness of the physicians about telemedicine. Materials and Methods: This was a survey study of physicians from different specialties who provided patient care during the pandemic in Turkey. A total of 824 physicians responded to questionnaire, which consisted of 5 sections: (1) demographic characteristics; (2) anxiety level; (3) knowledge; (4) perspective; and (5) willingness to use telemedicine. Results: Fifty-six percent of the participants were found to experience mild-to-severe anxiety during the pandemic. It was found that the early career physicians most likely report anxiety about COVID-19 (p = 0.012). Physicians working in training and research hospital settings had higher Beck Anxiety Inventory scores compared to their colleagues working in private health care institutions (p = 0.011). Anxiety levels of physicians were not affected by working experience, existence of comorbidities, or living conditions of the participants (p = 0.138, p = 0.317, and p = 0.123, respectively). The results showed that the participants had a low level of knowledge about telemedicine. Only 61.1% of the physicians stated that they had heard of telemedicine before. The physicians who experienced telemedicine before (N = 76, 9.2% of all the participants) were more likely to find telemedicine beneficial both in pandemic (p < 0.001) and postpandemic period (p = 0.002). Conclusions: About half of our physicians had different levels of anxiety during the pandemic, and this anxiety seemed to be more related to infecting their relatives. Participants thought that providing health care services with telemedicine during the pandemic period would be beneficial and reduce the spread of hospital-acquired COVID-19. However, there was no consensus among the participants regarding the use of telemedicine in the postpandemic period.