RESUMEN
OBJECTIVE: To examine the integrity (pH, bacterial counts, host defense factors, nutrient contents, and osmolality) of freshly expressed and previously refrigerated human milk subjected to long-term freezer storage. STUDY DESIGN: Mothers donated 100 mL of freshly expressed milk. Samples were divided into baseline, storage at -20°C (fresh frozen) for 1, 3, 6, and 9 months, and prior storage at +4°C for 72 hours (refrigerated frozen) before storage at -20°C for 1 to 9 months. Samples were analyzed for pH, total bacterial colony count, gram-positive and gram-negative colony counts, and concentrations of total protein, fat, nonesterified fatty acids, lactoferrin, secretory IgA, and osmolality. RESULTS: Milk pH, total bacterial colony count, and Gram-positive colony counts decreased significantly with freezer storage (P < .001); bacterial counts decreased most rapidly in the refrigerated frozen group. The gram-negative colony count decreased significantly over time (P < .001). Nonesterified fatty acid concentrations increased significantly with time in storage (P < .001). Freezing for up to 9 months did not affect total protein, fat, lactoferrin, secretory IgA, or osmolality in either group. CONCLUSIONS: Freezer storage of human milk for 9 months at -20°C is associated with decreasing pH and bacterial counts, but preservation of key macronutrients and immunoactive components, with or without prior refrigeration for 72 hours. These data support current guidelines for freezer storage of human milk for up to 9 months for both freshly expressed and refrigerated milk.
Asunto(s)
Congelación , Leche Humana/química , Refrigeración/estadística & datos numéricos , Recuento de Colonia Microbiana , Femenino , Humanos , Proteínas de la Leche/análisis , Leche Humana/microbiología , Madres , Factores de TiempoRESUMEN
Numerous short and long-term benefits of breastfeeding the full-term infant have been acknowledged. The use of human milk in the neonatal intensive care unit (NICU) is emerging as the beneficial effects are being realized. Challenges facing the practitioners today include providing optimal storage and processing strategies for mother's own milk (MOM), as well as offering an alternative when MOM is unavailable or supply is insufficient. We review the health benefits of human milk for the ELBW infant and the strategies for optimal use of the milk in the NICU.