RESUMEN
A low-Mr copper (Cu) and zinc (Zn)-binding protein was present in the liver of a control water lizard Triturus pyrrhogaster. Cadmium (Cd) accumulated in the liver of the animals after intramuscular injections of Cd salt and was bound to Cu- and Zn-binding protein of a single isoform that had properties characteristic of metallothionein (MT). Effects of Cd loading on several essential elements in the liver and kidney were also determined.
Asunto(s)
Cadmio/metabolismo , Cobre/metabolismo , Hígado/metabolismo , Metalotioneína/metabolismo , Triturus/metabolismo , Zinc/metabolismo , Animales , Cadmio/farmacología , Elementos Químicos/metabolismo , Riñón/metabolismoRESUMEN
Cadmium (Cd) was injected intramuscularly into adults (male) and larvae of the bullfrog, Rana catesbeiana. The chemical forms of Cd in the livers and the effect on several essential metal levels were determined by high performance liquid chromatography-atomic absorption spectrophotometry and inductively coupled plasma-atomic emission spectrophotometry, respectively. Metallothionein which binds cadmium, zinc and copper was induced in the liver and kidney of the adult bullfrog, and the protein was a mixture of two isoforms in both tissues. Although the two isoforms were also induced in the liver of the larva (tadpole), the relative ratio was the reverse of that in the adult.
Asunto(s)
Calcio/farmacología , Metalotioneína/metabolismo , Rana catesbeiana/metabolismo , Envejecimiento , Animales , Cromatografía Líquida de Alta Presión , Riñón/metabolismo , Larva/metabolismo , Hígado/metabolismo , Masculino , Metales/metabolismo , Espectrofotometría AtómicaRESUMEN
Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0 x 10(5) organisms per ml from infected mice, were 23-fold and 150-fold in the primary culture and the 3rd subculture, respectively. Even a single borrelia could propagate in this cultivation system. This system will be useful for immunological and physiological studies on uncultivable Borrelia strains.
Asunto(s)
Borrelia/crecimiento & desarrollo , Técnicas Microbiológicas , Animales , Borrelia/patogenicidad , Infecciones por Borrelia/patología , Células Cultivadas , Recuento de Colonia Microbiana , Medios de Cultivo , Femenino , Técnicas In Vitro , Ratones , Ratones Endogámicos BALB C , ConejosRESUMEN
Concentrations and contents of essential metals in the livers of prenatal and neonatal rats were determined simultaneously by inductively coupled plasma-atomic emission spectrometry. The concentrations and contents changed with development in a mode characteristic to the respective metals. Changes in the concentrations and contents of zinc and copper were correlated to those bound to metallothionein, the two metals being high in concentration during prenatal and neonatal periods. The relative ratio of the two isoforms of metallothionein changed with development, the form I being predominant in prenatal livers.