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1.
BMC Oral Health ; 22(1): 424, 2022 09 22.
Artículo en Inglés | MEDLINE | ID: mdl-36138429

RESUMEN

BACKGROUND: The COVID-19 pandemic challenged all healthcare providers including dental practitioners. This cross-sectional study aimed to investigate the dental practitioners' perceptions and attitudes towards the impacts of COVID-19 on their professional practice, career decision and patient care. METHODS: Data was collected from dental practitioners registered in New South Wales (NSW), Australia using an online survey. RESULTS AND CONCLUSION: Responses received from 206 dental practitioners revealed their perceptions and attitudes towards COVID-19 infection risk, clinical guidelines, and measures adopted to deliver patient care. Majority of participants perceived the risk of infection in dentistry was higher compared with other health professionals. Most dental practices have followed guidelines received from professional associations and adopted multiple measures such as providing hand sanitizer, social distancing, and risk screen, to ensure safe delivery of oral health care. Over 80% of dental practitioners raised concerns on patients' accessibility to dental care during the pandemic. Despite tele-dentistry was introduced, almost half of the participants did not recognize tele-dentistry as an effective alternative. Moreover, negative impacts of COVID-19 pandemic on dental practitioner's professional career have been reported, including lower practice safety, reduction in working hours and income. Noteworthy, one quarter of participants even considered changing their practice environment, moving sectors or even leaving their career in dentistry. However, majority of the dental practitioners are willing to stay in their current practice environment and continue their career in dentistry. Our observations demonstrate the systematic disruption to dental practice faced in Australia due to the COVID-19 pandemic. Providing dental practitioners with timely educational training and support is important to minimise negative impacts of the challenges and to optimise dental care.


Asunto(s)
COVID-19 , Desinfectantes para las Manos , Estudios Transversales , Odontología , Odontólogos , Humanos , Pandemias , Rol Profesional , Encuestas y Cuestionarios
2.
Bioethics ; 35(7): 646-651, 2021 09.
Artículo en Inglés | MEDLINE | ID: mdl-34184785

RESUMEN

How should the dental profession of the 21st century frame its interactions with the society it is tasked to care for? What has this relationship looked like in the past, and what does it look like today? In this article, we examine these and other issues through the framework of the social contract, with a focus on exploring how social justice fits within the transaction of duties between the dental profession and society. We begin by describing the social contract and how this is uniquely defined within the context of dentistry; specifically, how the context of dentistry as, in part, an aesthetically driven discipline, impacts the social contract. We then consider how the nature of the profession's relationship with society, and the orientation by which it provides its services (which is sometimes critically unclear in its definitional terms), impacts the profession's contribution to ensuring social justice in oral health and oral healthcare. Through examining the nature of how the social contract has shifted (for example, by the attenuation of professional monopolies), we also ask whether this is evidence of a loss of confidence in the dental profession as an altruistic institution. We end by suggesting that the dental profession must engage with the tenets of social justice within the social contract. Failure to do so is likely to lead to erosion of dentistry as a high-status profession and societal willingness to seek solutions to oral health needs from other professional and non-professional sources.


Asunto(s)
Altruismo , Justicia Social , Atención a la Salud , Odontología , Humanos , Salud Bucal
3.
BMC Med Educ ; 21(1): 164, 2021 Mar 17.
Artículo en Inglés | MEDLINE | ID: mdl-33731100

RESUMEN

BACKGROUND: Social conditions have a significant impact on the health of individuals and populations. While the dental curriculum is focused on teaching students about the diseases that affect the dentition and oral structures from a biomedical perspective, education about the social determinants of health is frequently regarded as less important. Thus, it occupies a smaller and disconnected part of the dental curriculum. The aim of this study was to explore the ways dental students conceptualised the social determinants of health after one year in dental school. METHODS: Reflective statements written by first year dental students at the end of the first year of study were collected. This qualitative study has an interpretivist basis and a thematic analysis of the reflections was conducted by two researchers. Metzl's structural competencies were used as a further analytic device. RESULTS: Four inter-related themes were identified: First, professional attitudes taken up by students influence their conceptions. Second, structural barriers to students understanding social determinants of health generate partial understandings. Thirdly, the social gulf that exists between the student body and people of different circumstances provides context to understanding the student's perspectives. Finally, we described how students were learning about the social determinants of health over the academic year. CONCLUSIONS: Dental students face several challenges when learning about the social determinants of health, and translating these learnings into actions is perhaps even more challenging. Metzl's structural competencies provide a framework for advancing students' understandings. One of the most important findings of this research study is that coming to an understanding of the social determinants of health requires sustained attention to social theories, practical experiences as well as institutionalised attitudes that could be achieved through an intentional curriculum design.


Asunto(s)
Formación de Concepto , Estudiantes de Odontología , Curriculum , Educación en Odontología , Humanos , Determinantes Sociales de la Salud
4.
BMC Med Ethics ; 21(1): 45, 2020 06 03.
Artículo en Inglés | MEDLINE | ID: mdl-32493373

RESUMEN

BACKGROUND: What can the analysis of the evolution of a code of ethics tell us about the dental profession and the association that develops it? The establishment of codes of ethics are foundational events in the social history of a profession. Within these documents it is possible to find statements of values and culture that serve a variety of purposes. Codes of ethics in dentistry have not frequently presented as the subjects of analyses despite containing rich information about the priorities and anxieties within the profession's membership at the time that the code was written. MAIN TEXT: This essay uses critical discourse analysis to explore the 2012 and 2018 versions of the Code of Ethics produced by the New South Wales Branch of the Australian Dental Association. This method of discourse analysis examines contradictions between the discourses within the codes and how these relate to broader social realties that surround the dental profession in New South Wales. By analysing the 2012 and 2018 codes together, it is possible to understand how the dental profession views its commitments to society as established through the social contract. Through this assessment, it will be demonstrated that both codes suffer due to their failure to consider the public as a key stakeholder in the creation and curation of the Code of Ethics and how this this relates intimately with the social contract between the profession and the public. CONCLUSION: Without the public being the central consideration, both codes amount to declarations of professional privilege and dominance. Although the more recent 2018 Code of Ethics demonstrates insight into the changes in public trust placed in the professions, this analysis shows that that the current code of ethics is still reluctant to recognise and engage with the public as an equal stakeholder in the planning and provision of oral health care and the development of the profession's values and cultural trajectory.


Asunto(s)
Códigos de Ética , Ética Odontológica , Australia , Humanos , Nueva Gales del Sur
5.
Med Health Care Philos ; 23(2): 261-268, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31538275

RESUMEN

Commercialisation and consumerism have had lasting and profound effects upon the nature of oral health and how dental services are provided. The stigma of a spoiled dental appearance, along with the attraction of the smile as a symbol of status and prestige, places the mouth and teeth as an object and product to be bought and sold. How the dental profession interacts with this acquired status of the mouth has direct implications for the professional status of dentistry and the relationship between the profession and society. This essay examines the mouth's developing position as a symbol of status and prestige and how the dental profession's interaction and response to this may have important effects on the nature of dentistry's social contract with society. As rates of dental disease reduce in higher socioeconomic groups, dentistry is experiencing a reorientation from being positioned within a therapeutic context, to be increasingly viewed as body work. This is not in of itself problematic; as a discipline dentistry places a very high value upon the provision of enhanced or improved aesthetics. This position changes when the symbolic exchange value of an aesthetic smile becomes the main motivation for treatment, encouraging a shift towards a commercialised model of practice that attenuates professional altruism. The dental profession should not welcome the association of the mouth as a status and prestige symbol lightly; this article examines how this paradigm shift might impact upon the social contract and dentistry's professional status.


Asunto(s)
Industria de la Belleza , Odontología , Boca , Distancia Psicológica , Ética Odontológica , Humanos , Motivación , Filosofía Médica , Profesionalismo
6.
J Evid Based Dent Pract ; 20(1): 101421, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32381402

RESUMEN

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Conceptual framework explaining "preparedness for practice" of dental graduates: a systematic review. Mohan M, Ravindran TKS. J Dent Educ 2018;82(11):1194-1202. SOURCE OF FUNDING: None declared. TYPE OF STUDY: Systematic review.


Asunto(s)
Competencia Clínica , Educación en Odontología , Humanos
7.
J Evid Based Dent Pract ; 20(2): 101442, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32473810

RESUMEN

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Humanities in Predoctoral Dental Education: A Scoping Review. Marti KC, Mylonas AI, MacEachern M, Gruppen L. J Dent Educ 2019;83(10):1174-1198. SOURCE OF FUNDING: None declared. TYPE OF STUDY/DESIGN: A scoping review.


Asunto(s)
Curriculum , Humanidades , Educación en Odontología , Humanos
8.
Parasitology ; 146(4): 413-424, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30318029

RESUMEN

Giardia duodenalis is a ubiquitous flagellated protozoan parasite known to cause giardiasis throughout the world. Potential transmission vehicles for this zoonotic parasite are both water and food sources. As such consumption of water contaminated by feces, or food sources washed in contaminated water containing parasite cysts, may result in outbreaks. This creates local public health risks which can potentially cause widespread infection and long-term post-infection sequelae. This paper provides an up-to-date overview of G. duodenalis assemblages, sub-assemblages, hosts and locations identified. It also summarizes knowledge of potential infection/transmission routes covering water, food, person-to-person infection and zoonotic transmission from livestock and companion animals. Public health implications focused within the UK, based on epidemiological data, are discussed and recommendations for essential Giardia developments are highlighted.


Asunto(s)
Giardia lamblia/fisiología , Giardiasis , Animales , Giardiasis/diagnóstico , Giardiasis/epidemiología , Giardiasis/transmisión , Giardiasis/veterinaria , Humanos , Salud Pública , Factores de Riesgo , Reino Unido
9.
Intern Med J ; 49(8): 1022-1025, 2019 08.
Artículo en Inglés | MEDLINE | ID: mdl-31387139

RESUMEN

This update restates the legal limits of consent to medical treatment. It reviews the classic statement of law set down by the case of R v Brown, where 'proper medical treatment' was stated to be exempted from the ordinary laws of consent. This position has been recently developed further by the 2018 English case of R v BM where a non-medical body modification artist was convicted for having carried out substantive body modification procedures. This article considers how these developments may affect Australian law, particularly laws pertaining to cosmetic procedures.


Asunto(s)
Consentimiento Informado/legislación & jurisprudencia , Procedimientos de Cirugía Plástica/legislación & jurisprudencia , Cirugía Plástica/legislación & jurisprudencia , Australia , Humanos , Responsabilidad Legal
10.
J Evid Based Dent Pract ; 19(1): 95-97, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30926109

RESUMEN

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Informed consent in dental care and research for the older adult population: A systematic review. Mukherjee A, Livinski AA, Millum J, Chamut S, Boroumand SB, Iafolla TJ, Adesanya, MR, Dye BA. J Am Dent Assoc 2017; 148(4):211-20. SOURCE OF FUNDING: Mukherjee: NIDCR; Livinski: NIH Library; Millum: Fogarty International Center and NIH; Chamut: NIDCR; Boroumand: NIDCR consultant on Science and Policy; Iafolla: NIDCR; Adesanya: NIDCR; and Dye: NIDCR. TYPE OF STUDY/DESIGN: Systematic review.


Asunto(s)
Atención Odontológica , Consentimiento Informado , Anciano , Humanos , Políticas
11.
Bioethics ; 32(9): 602-610, 2018 11.
Artículo en Inglés | MEDLINE | ID: mdl-30194688

RESUMEN

Cosmetic dentistry is a divisive discipline. Within discourses that raise questions of the purpose of the dental profession, cosmetic dentistry is frequently criticised on the basis of it being classified as a non-therapeutic intervention. This article re-evaluates this assertion through examination of ethics of care of the self, healthcare definitions and the social purpose of dentistry, finding the traditional position to be wanting in its conclusions. The slide of dentistry from a healthcare vocation towards being a predominantly business-focused interaction between clinician and consumer conflicts with traditional notions of dentistry as a profession. Whilst it is undeniable that cosmetic dental treatment particularly lends itself to the commercial paradigm, this is not exclusive to this area of professional practice. The cultural basis of dental appearance and the potential of the dental profession to exert coercive pressure upon the public to undergo treatment that is based upon social norms is discussed. This essay concludes that cosmetic dentistry is undeniably part of the professional purpose of 21st Century dentistry. However, the caveat that may be placed upon this, is that this status is conditional upon the professional conduct of dental practitioners remaining resilient to commercial practices not compatible with professional obligations.


Asunto(s)
Atención Odontológica/ética , Odontólogos/ética , Estética Dental , Ética Odontológica , Profesionalismo/ética , American Dental Association , Humanos , Comercialización de los Servicios de Salud/ética , Responsabilidad Social , Estados Unidos
12.
J Law Med ; 25(4): 1134-1145, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29978691

RESUMEN

This article seeks to examine and compare the legal and professional positions of tooth whitening of three jurisdictions: the United Kingdom, Australia and New Zealand. Within each jurisdiction, a differing formula of legislation, case law and professional guidance dictates how tooth whitening is regulated and practised. Tooth whitening still holds curiosity as a procedure with regards to whether it does indeed warrant status as a professional activity and whether its practice should be limited to dental professionals. Through exploration of the differing positions of three different jurisdictions, it is possible to demonstrate that this issue is far greater than the common view within the profession that for reasons of public protection, access to tooth whitening should be controlled by the dental profession. The monopoly upon tooth whitening procedures that the dental profession often celebrates, where it exists, should be viewed to be fragile and not taken as an unending status quo.


Asunto(s)
Blanqueamiento de Dientes , Australia , Humanos , Jurisprudencia , Nueva Zelanda , Reino Unido
13.
J Law Med ; 25(3): 794-799, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29978668

RESUMEN

Within medicine, there has been a protracted conversation relating to the appropriateness of accepting gifts and incentives from industry, professional colleagues and from patients. The general principle and anxiety in this debate relates to answering the question of whether accepting gifts or incentives compromises a health professional's duty to provide quality care. Within the dental profession, there is noticeably less discussion as to the effects of gifts and incentives upon the practice of dentistry. Given that dentistry is, like medicine, part of health care this status quo is not one that should persist. The authors hope that this article will stimulate discussion around dentistry's relationship with those who might seek to make commercial benefit out of our practice and how dental professionals should respond to patients bearing gifts - letters to the editor are explicitly invited; personal communication to the authors to compile a follow-up publication is welcome.


Asunto(s)
Conflicto de Intereses , Industria Farmacéutica/ética , Donaciones , Motivación , Humanos , Industrias , Boca
14.
J Law Med ; 25(4): 1146-1156, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29978692

RESUMEN

There are established standards for the management of infection control in private dental practices, but there is currently no proactive legislation to oversee correct adherence to those standards. The Australian Health Service Safety and Quality Accreditation Scheme (Scheme) promotes a quality and safety management program that includes attention to the prevention of healthcare acquired infections, but adoption of the Scheme is not compulsory for all. A recent case brought before the NSW Civil and Administrative Tribunal demonstrated the seriousness of breaches of infection control standards and the considerable costs of managing the consequences. This article discusses the role of quality and safety assurance programs in the context of private dental practice regulation; compares the different quality and safety assurance schemes operating in Australia, Canada, and the United Kingdom; and argues that the compulsory adoption of an appropriate scheme which focuses on infection control would be in the public interest.


Asunto(s)
Acreditación , Control de Infecciones , Administración de la Práctica Odontológica , Australia , Canadá , Reino Unido
15.
Epidemiol Infect ; 145(8): 1584-1590, 2017 06.
Artículo en Inglés | MEDLINE | ID: mdl-28274291

RESUMEN

Giardia duodenalis and Cryptosporidium species are protozoan parasites capable of causing gastrointestinal disease in humans and animals through the ingestion of infective faeces. Whereas Cryptosporidium species can be acquired locally or through foreign travel, there is the mis-conception that giardiasis is considered to be largely travel-associated, which results in differences in laboratory testing algorithms. In order to determine the level of variation in testing criteria and detection methods between diagnostic laboratories for both pathogens across Scotland, an audit was performed. Twenty Scottish diagnostic microbiology laboratories were invited to participate with questions on sample acceptance criteria, testing methods, testing rates and future plans for pathogen detection. Reponses were received from 19 of the 20 laboratories representing each of the 14 territorial Health Boards. Detection methods varied between laboratories with the majority performing microscopy, one using a lateral flow immunochromatographic antigen assay, another using a manually washed plate-based enzyme immunoassay (EIA) and one laboratory trialling a plate-based EIA automated with an EIA plate washer. Whereas all laboratories except one screened every stool for Cryptosporidium species, an important finding was that significant variation in the testing algorithm for detecting Giardia was noted with only four laboratories testing all diagnostic stools. The most common criteria were 'travel history' (11 laboratories) and/or 'when requested' (14 laboratories). Despite only a small proportion of stools being examined in 15 laboratories for Giardia (2%-18% of the total number of stools submitted), of interest is the finding that a higher positivity rate was observed for Giardia than Cryptosporidium in 10 of these 15 laboratories. These findings highlight that the underreporting of Giardia in Scotland is likely based on current selection and testing algorithms.


Asunto(s)
Auditoría Clínica/normas , Criptosporidiosis/diagnóstico , Giardiasis/diagnóstico , Cromatografía de Afinidad , Criptosporidiosis/epidemiología , Criptosporidiosis/parasitología , Cryptosporidium/aislamiento & purificación , Notificación de Enfermedades , Giardia/aislamiento & purificación , Giardiasis/epidemiología , Giardiasis/parasitología , Humanos , Técnicas para Inmunoenzimas , Escocia/epidemiología
16.
Epidemiol Infect ; 145(14): 3007-3011, 2017 10.
Artículo en Inglés | MEDLINE | ID: mdl-28879824

RESUMEN

Giardiasis is a treatable disease, caused by the flagellated protozoan parasite, Giardia duodenalis (G. duodenalis). It is one of the most common enteric parasites found globally to cause gastrointestinal disturbances, and infections may result in long-term irritable bowel syndrome-like symptoms. It is a common misconception that giardiasis is associated with foreign travel, which results in locally acquired cases in the UK being underdiagnosed. This report highlights the findings from one large Scottish Health Board, arising from a change in testing methodology, which resulted in the screening of all stools submitted for enteric investigations for G. duodenalis. Previous selection criteria were restricted to patients with a travel history to specific regions of the world, or on the basis of certain clinical details. In this report, clinical details were recorded from samples shown to be positive using two methods: an ELISA-based antigen detection assay and microscopy. Clinical details were assessed for a total of 28 laboratory-confirmed positive cases against the original selection criteria. Twenty-six cases (93%) would have been excluded from Giardia testing if the previous selection criteria had been applied. Although nine cases stated foreign travel, only two had been to regions deemed to be 'high risk'. Therefore, those seven cases that travelled to perceived 'low-risk' regions would have been excluded from testing for this reason. This summary highlights the need for significant improvements to the selection criteria for Giardia testing. Laboratories should be encouraged towards the testing of all routinely submitted stools for this neglected pathogen to ensure cases that are acquired locally are properly identified and treated effectively.


Asunto(s)
Notificación de Enfermedades/métodos , Giardia lamblia/aislamiento & purificación , Giardiasis/diagnóstico , Salud Pública/métodos , Adolescente , Adulto , Anciano , Niño , Preescolar , Notificación de Enfermedades/normas , Ensayo de Inmunoadsorción Enzimática , Heces/parasitología , Femenino , Giardiasis/parasitología , Humanos , Masculino , Microscopía , Persona de Mediana Edad , Escocia , Adulto Joven
17.
Epidemiol Infect ; 143(6): 1219-24, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25185671

RESUMEN

Cryptosporidium hominis is one of the most prevalent protozoan parasites to infect humans where transmission is via the consumption of infective oocysts. This study describes sporadic cases in addition to the molecular diversity of outbreak cases in Scotland using the glycoprotein-60 subtyping tool. From a total of 187 C. hominis isolates, 65 were subjected to further molecular analysis and 46 were found to be the common IbA10G2 subtype. Unusual subtypes included four isolates belonging to the Ia family (IaA14R3, n = 12; IaA14R2, n = 1; IaA9G3, n = 1; IaA25R3, n = 2), two from the Id family (IdA24, n = 1; IdA17, n = 1) and one belonging to the Ie family, namely IeA11G3T3. These data contribute significantly to our knowledge and understanding of the molecular diversity of C. hominis isolates from outbreak investigations involving Scottish residents which will be beneficial for the management of future outbreaks.


Asunto(s)
Cryptosporidium/genética , Adolescente , Adulto , Factores de Edad , Niño , Preescolar , Criptosporidiosis/epidemiología , Criptosporidiosis/parasitología , Brotes de Enfermedades/estadística & datos numéricos , Femenino , Variación Genética , Humanos , Masculino , Persona de Mediana Edad , Escocia/epidemiología , Adulto Joven
18.
Parasitology ; 142(2): 318-25, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25244937

RESUMEN

Cryptosporidium parvum (C. parvum) is one of the most prevalent protozoan pathogens responsible for inducing human and animal disease worldwide. In this study, the glycoprotein-60 (gp60) subtyping tool was employed to assess the molecular diversity of C. parvum from human feces throughout Scotland during potential outbreaks. Over a 24-month period, microscopy analysis revealed 1139 positive feces containing Cryptosporidium species with 256 identified by molecular methods specifically as C. parvum. Cryptosporidium parvum was shown to be more prevalent in rural areas of Scotland and subtyping of 87 isolates demonstrated the predominant family as IIa, which occurred in 94% (n=82) of isolates. The IIaA15G1R1 subtype was most common, being isolated from 47% (n=41) of Scottish human cases. Non-IIa strains constituted a total of 5 isolates and included subtypes from the IIc, IId and IIg families. This information contributes significantly to existing knowledge and understanding of C. parvum subtypes in Scotland which is vital in assisting with the management of future local and national outbreaks.


Asunto(s)
Criptosporidiosis/epidemiología , Criptosporidiosis/parasitología , Cryptosporidium parvum/genética , Cryptosporidium parvum/aislamiento & purificación , Genotipo , Humanos , Escocia/epidemiología , Estaciones del Año , Especificidad de la Especie , Factores de Tiempo
19.
Dent Update ; 41(1): 46-8, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24640477

RESUMEN

UNLABELLED: The different ways that clinicians perceive adult patients with dental phobia is varied and diverse. From treating the dental phobia as a separate illness to dismissing it as a neurosis, sometimes little consequence is attached to its existence. True dental phobia is classed as a psychological illness and therefore comes under the remit and guidance of the Mental Capacity Act 2005. Failure to assess the impact of dental phobia upon an individual's capacity to consent could lead to allegations of negligence or even assault. CLINICAL RELEVANCE: This paper highlights the importance of considering the capacity to consent of some of the most vulnerable dental


Asunto(s)
Atención Odontológica/legislación & jurisprudencia , Consentimiento Informado/legislación & jurisprudencia , Competencia Mental/legislación & jurisprudencia , Adulto , Toma de Decisiones , Ansiedad al Tratamiento Odontológico/psicología , Relaciones Dentista-Paciente , Derechos Humanos/legislación & jurisprudencia , Humanos , Mala Praxis/legislación & jurisprudencia , Derechos del Paciente/legislación & jurisprudencia , Reino Unido , Poblaciones Vulnerables/legislación & jurisprudencia
20.
Appl Environ Microbiol ; 79(7): 2240-6, 2013 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-23354707

RESUMEN

This is the first report to characterize the genotypes and subtypes of Cryptosporidium species infecting a geographically isolated population of feral Soay sheep (Ovis aries) on Hirta, St. Kilda, Scotland, during two distinct periods: (i) prior to a population crash and (ii) as host numbers increased. Cryptosporidium DNA was extracted by freeze-thawing of immunomagnetically separated (IMS) bead-oocyst complexes, and species were identified following nested-PCR-restriction fragment length polymorphism (RFLP)/PCR sequencing at two Cryptosporidium 18S rRNA loci. Two hundred fifty-five samples were analyzed, and the prevalent Cryptosporidium species in single infections were identified as C. hominis (11.4% of all samples tested), C. parvum (9%), C. xiaoi (12.5%), and C. ubiquitum (6.7%). Cryptosporidium parvum was also present with other Cryptosporidium species in 27.1% of all samples tested. Cryptosporidium parvum- and C. hominis-positive isolates were genotyped using two nested-PCR assays that amplify the Cryptosporidium glycoprotein 60 gene (GP60). GP60 gene analysis showed the presence of two Cryptosporidium genotypes, namely, C. parvum IIaA19G1R1 and C. hominis IbA10G2. This study reveals a higher diversity of Cryptosporidium species/genotypes than was previously expected. We suggest reasons for the high diversity of Cryptosporidium parasites within this isolated population and discuss the implications for our understanding of cryptosporidiosis.


Asunto(s)
Criptosporidiosis/veterinaria , Cryptosporidium/clasificación , Cryptosporidium/genética , Variación Genética , Enfermedades de las Ovejas/parasitología , Animales , Análisis por Conglomerados , Criptosporidiosis/parasitología , Cryptosporidium/aislamiento & purificación , ADN Protozoario/química , ADN Protozoario/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Genes de ARNr , Genotipo , Glicoproteínas/genética , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Proteínas Protozoarias/genética , ARN Protozoario/genética , ARN Ribosómico 18S/genética , Escocia , Análisis de Secuencia de ADN , Ovinos
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