RESUMEN
Fifty-seven genital tracts of regularly slaughtered culled Piedmontese cows, aged 7.4 ± 4.3 years (mean ± SD), range: 2.6-15.6 years, were grossly and microscopically examined. DNA extracted from oviducts was subjected to PCR to evaluate the presence of Chlamydia spp. The 15 PCR-positive oviducts were subjected to Sanger sequencing and showed the presence of Chamydia abortus, with an identity range between 99 and 100%. Nine of the PCR-positive samples belonged to the 24 animals with a normal macroscopic appearance of the whole genital tract (percentage of positive oviducts in normal genital tracts 9/24 = 37.5%), while six belonged to the 33 genital tracts with lesions in one or more organs (percentage of positive oviducts in pathological genital tracts 6/33 = 18.1%); of these, a single animal had salpingitis. The detection of C. abortus in bovine oviducts is of particular interest because it has never been previously investigated or reported.
Asunto(s)
Enfermedades de los Bovinos/microbiología , Infecciones por Chlamydia/veterinaria , Chlamydia/aislamiento & purificación , Oviductos/microbiología , Animales , Bovinos , Chlamydia/clasificación , Infecciones por Chlamydia/diagnóstico , FemeninoRESUMEN
An exploratory laparotomy on a mixed-breed bitch of an estimated age of 5 years revealed that she had undergone ovariectomy in the past, but a cystic structure was present in the area of the right ovary and a whitish mass, approximately 3 cm in diameter, in the area of the left ovary. These structures were removed together with an apparently normal uterus. Histological examination of the cyst showed a thin layer of connective tissue, while the left ovarian mass revealed ovarian tissue and highly differentiated nervous tissue, confirmed through immunohistochemistry. A presumptive diagnosis of mature ovarian teratoma was made. Although teratomas generally contain recognizable elements from more than one of the three germ cell layers, they can also be monophasic, when there is only one germ layer component. Ovarian teratomas are rare in the dog and never before have been reported in an ovarian fragment.
Asunto(s)
Enfermedades de los Perros/patología , Neoplasias Ováricas/veterinaria , Teratoma/veterinaria , Animales , Enfermedades de los Perros/cirugía , Perros , Femenino , Neoplasias Ováricas/patología , Neoplasias Ováricas/cirugía , Teratoma/patología , Teratoma/cirugíaRESUMEN
Six veal calves were medicated with clenbuterol at 20 micrograms kg bodyweight-1 day-1 for 42 days before they were slaughtered, to evaluate the lesions and residues in target organs. Compared with six unmedicated calves the most noticeable changes were tracheal dilatation, decreased uterine weight, slight mucous hypersecretion in the uterus and vagina and depletion of liver glycogen. The highest concentrations of clenbuterol (62 to 128 ng/g-1) were recorded in the choroid/retina, and the aqueous humour had the lowest concentration (0.5 to 2.4 ng ml-1). The residue concentrations were higher than the maximum residue level set for clenbuterol (0.5 ng g-1).
Asunto(s)
Bovinos/metabolismo , Clenbuterol/farmacocinética , Clenbuterol/toxicidad , Residuos de Medicamentos/análisis , Animales , Peso Corporal/efectos de los fármacos , Enfermedades de los Bovinos/inducido químicamente , Enfermedades de los Bovinos/patología , Femenino , Genitales Femeninos/efectos de los fármacos , Genitales Femeninos/patología , Hígado/efectos de los fármacos , Hígado/patología , Tamaño de los Órganos/efectos de los fármacos , Sistema Respiratorio/efectos de los fármacos , Sistema Respiratorio/patologíaRESUMEN
The prevalence of Chlamydophila felis and feline herpesvirus 1 (FHV-1) infection in cats with conjunctivitis in northern Italy was investigated by conventional polymerase chain reaction (PCR) testing. In cats with conjunctivitis, C felis and FHV-1 were detected in 14 of 70 (20%) and in 23 of 70 (33%) animals, respectively. None of the 35 control cats were positive for C felis, whereas 7 (20%) of these cats were positive for FHV-1. Mixed infections were present in 5 of 70 cats (7%). Cats positive for C felis were significantly younger than control animals (P = .02), whereas no significant age differences were observed between FHV-1-positive cats and control cats (P = .41) or between FHV-1-positive animals and C felis-positive animals (P = .16). Cats sampled during acute-phase conjunctivitis were also investigated for the presence of C felis by conjunctival scrapings. In this acute phase, substantial agreement was found when comparing the results of the 2 methods (K = .80). The association between PCR results and conjunctivitis was evaluated for the 2 pathogens. The presence of C felis was significantly associated with conjunctivitis (P = .004), whereas the detection of FHV-1 did not significantly correlate with the clinical sign (P = .25), suggesting that, by itself. PCR is not suitable for the diagnosis of FHV-1-related conjunctivitis.
Asunto(s)
Enfermedades de los Gatos/microbiología , Infecciones por Chlamydophila/veterinaria , Chlamydophila/aislamiento & purificación , Conjuntivitis/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesviridae/aislamiento & purificación , Animales , Enfermedades de los Gatos/epidemiología , Gatos , Chlamydophila/genética , Infecciones por Chlamydophila/epidemiología , Infecciones por Chlamydophila/microbiología , Infecciones por Chlamydophila/virología , Conjuntiva/microbiología , Conjuntiva/virología , Conjuntivitis/epidemiología , Conjuntivitis/microbiología , Conjuntivitis/virología , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Viral/química , ADN Viral/genética , Femenino , Herpesviridae/genética , Infecciones por Herpesviridae/epidemiología , Infecciones por Herpesviridae/microbiología , Infecciones por Herpesviridae/virología , Italia/epidemiología , Masculino , Reacción en Cadena de la Polimerasa/veterinaria , Estadísticas no ParamétricasRESUMEN
Pathologic findings, lectin histochemistry, and nuclear estrogen receptors were studied in the reproductive organs of gilts treated with clenbuterol. A ration containing 1 ppm of clenbuterol was fed for 40 days to four Landrace x Large white, 9-month-old gilts, weighing 134 to 172 kg at slaughter (gilt Nos. 5-8). Four gilts (Nos. 1-4) served as controls. Treated animals had macroscopic lesions characterized by microcystic ovaries and uterine atrophy. Histopathologic lesions included atretic degeneration of many ovarian follicles, complete absence of functional corpora lutea, a reduction in the number of endometrial glands, and a decrease in cytoplasmic volume of endometrial and glandular epithelial cells. In ovaries, uterus, and vagina lectin histochemistry, performed with thirteen different biotinylated lectins, revealed a different staining distribution between control and treated gilts. The binding pattern of Ricinus communis agglutinin-I (RCA-I) and -II (RCA-II) in the ovaries of control gilts, displayed labeling of cytoplasm in theca interna cells of Graafian follicles. There was no labeling of the same cells in treated gilts. Labeling patterns with Griffonia simplicifolia agglutinin-I (GS-I), Phaseolus vulgaris agglutinin (PHA), RCA-I and RCA-II documented a difference in the vascularity of the theca interna between Graafian follicles of control and treated gilts. The GS-I and Ulex europaeus agglutinin-I (UEA-I) binding patterns in uterus and vagina of treated gilts when compared to control gilts suggested that there was a block of the cycling activity in the proliferative stage. Immunohistochemical staining for estrogen receptors in the endometrium was positive in all but one treated gilts, and negative to weakly positive in control gilts. Serum progesterone concentrations were decreased in treated animals when compared to control; estradiol concentrations were similar in both group of gilts. Cystic ovaries, uterine atrophy, and reduction in progesterone concentrations suggested that clenbuterol changed ovarian hormonal activity in treated animals.