RESUMEN
1. The distribution of HMG-2 protein was followed in unfractionated rat brain cells at different stages of development. Its amount gradually decreased and reached the lowest level in the terminally differentiated and non-proliferating cells. 2. In isolated oligodendrocyte nuclei the changes in the content of HMG-2 followed the same pattern of distribution which corresponded to their stage of development and proliferative activity, while in the terminally differentiated and non-proliferating cortical neurons a substantial amount of HMG-2 protein was present up to the twenty-eighth postnatal day. 3. In the presence of anti-HMG-2 antibodies the DNA synthetic activity of oligodendrocyte nuclei in vitro was significantly decreased. The treatment with antibodies affected mainly the DNA replicative activity of the nuclei, while their DNA repair activity remained unchanged.