PFAS ghosts: how to identify, evaluate, and exorcise new and existing analytical interference.

With increasing public awareness of PFAS, and their presence in biological and environmental media across the globe, comes a matching increase in the number of PFAS monitoring studies. As more matrices and sample cohorts are examined, there are more opportunities for matrix interferents to appear as PFAS where there are none (i.e., "seeing ghosts"), impacting subsequent reports. Addressing these ghosts is vital for the research community, as proper analytical measurements are necessary for decision-makers to understand the presence, levels, and potential risks associated with PFAS and protect human and environmental health. To date, PFAS interference has been identified in several matrices (e.g., food, shellfish, blood, tissue); however, additional unidentified interferents are likely to be observed as PFAS research continues to expand. Therefore, the aim of this commentary is several fold: (1) to create and support a publicly available dataset of all currently known PFAS analytical interferents, (2) to allow for the expansion of that dataset as more sources of interference are identified, and (3) to advise the wider scientific community on how to both identify and eliminate current or new analytical interference in PFAS analyses.

Does soil track-in contribute to house dust concentrations of perfluoroalkyl acids (PFAAs) in areas affected by soil or water contamination?

The Minnesota Department of Health measured levels of perfluoroalkyl acids (PFAAs) in house dust at homes in communities impacted by PFAA-contaminated soil and drinking water to determine whether PFAAs in soil outside the home are associated with concentrations in dust. House dust samples from both interior living spaces and entryways to the yard were collected and analyzed separately based on the presumption that PFAAs in entryway dust may better reflect "track-in" of PFAAs into the home from contaminated soil or lawns irrigated with contaminated water. PFAA detections and concentrations in living rooms were significantly higher compared to entryways; and concentrations in both sampling locations were higher than corresponding soil concentrations, suggesting that interior sources were the main contributors to PFAAs in house dust. PFAA dust concentrations in entryways were significantly associated with living room dust levels for all analytes except PFBA. Relationships between entryway dust and soil were only seen for one PFAA (PFOA). However, median concentrations of PFOA in entryway and living room dust were 35 and 70 times higher (respectively) than in soil, which highlights the lack of importance of PFAA soil track-in as a contributor to dust concentration in this setting. Due to the small sample size, larger scale studies are needed to further assess the potential for migration of PFAA contaminated soil to indoor dust.

Occurrence of perfluoroalkyl substances (PFAS) in garden produce at homes with a history of PFAS-contaminated drinking water.

The decades-long disposal of manufacturing waste containing perfluoroalkyl substances (PFAS) in landfills resulted in contamination of groundwater serving as the drinking water supply for the eastern Twin Cities metropolitan region. While measures were taken to reduce the levels of PFAS in the drinking water, questions remained about possible non-drinking water pathways of exposure in these communities. The Minnesota Department of Health (MDH) investigated whether PFAS in water used for yard and garden irrigation results in elevated concentrations of PFAS in soil and home-grown produce. In 2010, samples of outdoor tap water, garden soil, and garden produce were collected at homes impacted by the contamination and analyzed for several PFAS. Perfluorobutanoic acid (PFBA) was the primary PFAS present in water, followed by perfluoropentanoic acid (PFPeA). Although PFBA, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were present in 100% of soil samples at higher concentrations compared to other PFAS, only PFBA was readily translocated to plants. Significant determinants of PFBA concentration in produce were the amount of PFBA applied to the garden via watering and the type of produce tested. Results from this real-world study are consistent with experimental findings that short-chain PFAS have the highest potential to translocate to and bioaccumulate in edible plants. These findings are globally relevant, as short-chain PFAS serve as commercial substitutes for longer-chain compounds and are increasingly detected in water due to their relatively high solubility and mobility.