Actionable diagnosis of neuroleptospirosis by next-generation sequencing.

A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were negative. Targeted antimicrobial agents were administered, and the patient was discharged home 32 days later with a status close to his premorbid condition. Polymerase-chain-reaction (PCR) and serologic testing at the Centers for Disease Control and Prevention (CDC) subsequently confirmed evidence of Leptospira santarosai infection.

Virus/allergen interactions in asthma.

Understanding the underlying mechanisms that cause and exacerbate allergic asthmatic disease is of great clinical interest. Clinical studies have revealed that allergies and viral respiratory illnesses are strongly linked to the inception and exacerbation of asthma, and suggest the possibility that there are interactive inflammatory mechanisms. Recent work has revealed a number of mechanisms of virus and allergen cross-talk that may play a role in the pathophysiology of allergic asthma, including (1) deficiency in virus-induced interferon responses, (2) defective epithelial barrier function, (3) increased release of epithelium-derived cytokines (e.g., thymic stromal lymphopoietin (TSLP), interleukin (IL)-25, IL-33), (4) dysregulation of lymphocytes [e.g., innate lymphoid cells (ILCs), regulatory T cells (Tregs)], and (5) altered activation of purinergic receptors. One or more of these processes may provide targets for new therapeutics to treat allergic asthma and prevent disease exacerbation.

Perceptions about water and increased use of bottled water in minority children.

OBJECTIVE: To describe bottled water use and beliefs and attitudes about water among parents of children from different racial/ethnic groups. DESIGN: Cross-sectional survey. SETTING: Urban/suburban emergency department. PARTICIPANTS: Parents of children treated between September 2009 and March 2010. MAIN OUTCOME MEASURES: The respondents completed a questionnaire in English or Spanish, describing their use of bottled water and tap water for their children and rating their agreement with a series of belief statements about bottled water and tap water. Logistic regression was used to evaluate the association between bottled water use and beliefs and demographic characteristics with odds ratios (ORs). RESULTS: A total of 632 surveys were completed (35% white, 33% African American, and 32% Latino respondents). African American and Latino parents were more likely to give their children mostly bottled water; minority children were exclusively given bottled water 3 times more often than non-Latino white children (24% vs 8%, P < .01). In logistic regression analysis, the following factors were independently associated with mostly bottled water use: belief that bottled water is safer (OR, 2.4), cleaner (OR, 2.0), better tasting (OR, 2.8), or more convenient (OR, 1.7). After other factors were adjusted for, race/ethnicity, household income, and prior residence outside the United States were not associated with bottled water use. CONCLUSIONS: Minority parents are more likely to exclusively give bottled water to their children. Disparities in bottled water use are driven largely by differences in beliefs and perceptions about water. Interventions to reduce bottled water use among minority families should be based on knowledge of the factors that are related to water use in these communities.