A guanidinylated bioreducible polymer as a novel gene carrier to the corpus cavernosum of mice with high-cholesterol diet-induced erectile dysfunction.

A prerequisite for the successful clinical application of gene therapy in erectile dysfunction (ED) is the availability of safe and efficient gene delivery systems. The aim of this study was to examine the effectiveness of guanidinylated bioreducible polymer (GBP) polyplexes for gene delivery systems, which take advantage of the biodegradability of reducible disulfide bonds and the cell-penetrating ability of guanidine groups. For in vitro transfection experiments, we used mouse cavernous endothelial cells and A7r5 rat vascular smooth muscle cells. For in vivo experiments, we used a mouse model of hypercholesterolaemic ED in which 2-month-old male C57BL/6 mice were fed a diet containing 4% cholesterol and 1% cholic acid for 3 months. Animals or cells were treated with pCMV-Luc, poly(ethyleneimine) (PEI)25k/pCMV-Luc polyplex (weight ratio: 1) and GBP/pCMV-Luc polyplexes (weight ratio: 20, 40, 60 and 80). Gene expression was evaluated by luciferase assay, and the gene expression area was evaluated by immunohistochemistry. GBP had greater transfection efficiency as the weight ratio increased. GBP had sevenfold higher gene delivery efficiency in A7r5 cells at a weight ratio of 80 than did PEI25k. Moreover, the gene expression was more profoundly induced by GBP/pCMV-Luc than by pCMV-Luc in both the corpus cavernosum tissue of hypercholesterolaemic mice and in mouse cavernous endothelial cells, although the expression levels induced by the GBP gene delivery system were lower than those induced by the PEI25k gene delivery system. GBP revealed no considerable cytotoxicity to A7r5 cells and mouse cavernous endothelial cells (relative cell viability: 95 and 88% respectively), whereas PEI25k resulted in high cytotoxicity. Interestingly, immunofluorescent double staining revealed that luciferase expression induced by the GBP polyplex mainly overlapped with cavernous endothelial cells, but rarely with smooth muscle cells. The GBP-based non-viral gene expression system may be useful for the development of gene therapy in vasculogenic ED.

MUCOCELE OF THE APPENDIX: CASE REPORT / Мэс засал

Appendiceal mucocele is an obstructivedilatation of the appendix caused byintraluminal accumulation of mucoid material.It is a rare disease. The incidence is 0.2%to 0.3% of all appendectomied specimens.The brush size to 3 cm are considered to besmall, up to 6 cm - average and more than9 cm - giantCases: the patient 50 years old, man,place of residence: the city of UB songinokhairkhan duureg. Specialists of the driver,in the year immediately 2015,02,02 wasoperated with the diagnosis of acuteappendicitis. Complaints on admission:pain on the right side of the abdomen, anagging pain when moving and walking,stomach rumbling. History of the disease:the pain started with 2015,01,31 at 8 pmfrom the bottom of the abdomen. tookhome chloramphenicol but not helpedso 2015,02,02 year at 9 o’clock enteredvia ambulance. Medical examination: thesatisfactory condition of the active position,the skin clean, moist, blood pressure160/110, pulse 98 beats per minute Spo298%. 10*7,7*5,5 см size /Fig.1-2/ , white /Fig. 3-4/ , smooth surface /Fig.1-4/, insideslimy gray mucinous /Fig. 5-6/ bag /Fig. 5/above listed items on a standard attached,aims to study the histology. The result ofthe following: Mucocele of the appendixwith hyalinized wall and with the additionof acute inflammation with microabscesses.

Study on Hepatitis B and C exposure in nurses / Монголын Анагаах Ухаан

Introduction.M.Colombo, W.Lange studies showed that 30-40% of people became chronic after suffering fromHepatitis B and C virus, about 50% of chronic cases transformed into primary liver cancer. There arefew studies in our country were conducted on hepatitis among healthcare professionals, particularnursing personnel.Goal.To identify antigens and antibodies of hepatitis B and C virus among nursesMaterials and Method.We carried out cross-sectional study among selected nurses, to determine surface antigen of hepatitisB virus and antibodies to hepatitis C virus. For identification of these antibodies and antigen, andvalidation of results Serodia tests from Fujinebo Company (Japan) and Beringnost (Germany) wereused respectively.Results.There were 598 nurses from the State Central Clinical Hospital, Shastin’s State Hospital, Hospitalof Military of Justice and Internal Affairs, and the National Center of Maternal and Child Health, whoparticipated in the study. From 5 hospitals a 598 nurses surveyed and revealed the hepatitis B virussurface antigen positive 18.9%, hepatitis C virus antibodies in 23.2%, B and C viruses detected by1.2% combined.Conclusion.The study identified that 43.2 percent of nurses surveyed on hepatitis B and C viruses were detected;it shows a high prevalence among the nurses. There is an urgent need to provide knowledge tomedical personnel regarding standards during procedures, concerning hepatitis infections, monitoringand improve technology used during procedures.