Expression of budding uninhibited by benzimidazoles-1 and mitotic arrest deficient-2 in endometrial carcinoma and its significance.

OBJECTIVE: The aim of this study was to explore the expression of budding uninhibited by benzimidazoles-1 (Bub1) and mitotic arrest deficient-2 (Mad2) in endometrial carcinoma and its significance. MATERIALS AND METHODS: The expression of Bub 1 and Mad2 in 30 human normal endometrial tissues (group A), 30 complexly-hyperplastic endometrial tissues (group B), and 63 endometrial carcinoma tissues (group C) was observed using immunohistochemistry (the streptavidin-peroxidase method). RESULTS: The positive expression rates of Bub1 in groups A, B, and C were 86.67%, 56.67%, and 28.57%, respectively. The positive rate of Bub1 protein was correlated with the differentiation degree and clinical stage of endometrial carcinoma (p < 0.05) other than lymph node metastasis (p > 0.05): A higher differentiation degree and a more advanced stage of endometrial carcinoma indicated a higher positive rate of Bub1 protein. The positive rates of Mad2 protein in groups A, B, and C were 23.33%, 56.67%, and 85.71%, respectively. The positive rate of Mad2 protein was correlated with the differentiation degree of endometrial carcinoma (p < 0.05) other than its clinical stage and lymph node metastasis (p > 0.05): A lower differentiation degree indicated a higher positive rate of Mad2 protein. Bub1 and Mad2 proteins were negatively correlated in the endometrial carcinoma tissues (r = - 0.719, p < 0.001). CONCLUSION: Bub1 and Mad2 proteins interact with each other. They may play an important role in the initiation and development of endometrial carcinoma.

Clinical analysis of 65 cases of laparoscopic treatments on tubal infertility caused by tubal distortion.

OBJECTIVE: This study aims to investigate the clinical value of laparoscopic treatment on tubal infertility caused by tubal distortion. MATERIALS AND METHODS: A total of 65 cases of patients with tubal infertility were divided into three groups based on tubal distortion degree, i.e., 21 cases had a minimum angle of tubal distortion < 45 degrees (A group), 39 cases had a distortion angle between 45 degrees and 90 degrees (B group), and five cases had a distortion angle between 90 degrees and 145 degrees (C group). The pregnancy outcome and the impact of tubal distortion degree on pregnancy outcome were analyzed 6 to 24 months after operation. RESULTS: The total pregnancy rate of these 256 cases were 43.75% with an intrauterine pregnancy rate of 40.23% and an ectopic pregnancy rate of 3.52%. In the simple distortion tubal infertility cases, the total pregnancy rate was 44.62%. In Group A, five cases became pregnant after operation (33.33%); in Group B, 19 cases (48.72%); and in Group C, three cases (60%). The differences in pregnancy rate between Groups A and B and Groups A and C were statistically significant (p < 0.05), whereas that between Groups B and C was not (p > 0.05). CONCLUSION: Tubal plastic surgery via laparoscopy is an effective way to treat infertility caused by tubal distortion by restoring the normal shape of oviducts, especially in cases when the minimum angle of tubal distortion is greater than 45 degrees.