Identification of a long non-coding RNA (LncMSEN2) from pearl oyster and its potential roles in exoskeleton formation and LPS stimulation.

Long non-coding RNAs (lncRNAs) play regulatory roles in various biological processes, including exoskeleton formation and immune response. The exoskeleton-based mantle-shell defense system is an important defense mechanism in shellfish. In this study, we found a novel lncRNA, herein formally named, LncMSEN2, from the pearl oyster Pinctada fucuta martensii, and its sequence was validated via polymerase chain reaction (PCR). LncMSEN2 was highly expressed in mantle tissues, especially in the central region (P < 0.05), and was also expressed in the pearl sac as detected by quantitative real-time PCR. In situ hybridization experiments revealed that LncMSEN2 had a strong positive signal in the inner and outer epidermal cells of the mantle pallial and central regions. RNA interference experiments showed that interference of LncMSEN2 expression with dsRNA in mantle tissues led to an abnormal crystal structure of the nacre. In addition, LncMSEN2 expression significantly increased 6 h after lipopolysaccharide stimulation in mantle tissues (P < 0.05). These results indicated that LncMSEN2 may be a novel regulator of the mantle-shell defense system of pearl oyster.

Association Between EEG Power During Sleep and Attention Levels in Patients with Major Depressive Disorder.

Purpose: Major depressive disorder (MDD) is associated with cognitive impairment through unclear mechanisms. We examined the relationship between sleep electroencephalogram (EEG) power and attention level in MDD. Patients and Methods: Forty-seven untreated patients with MDD and forty-seven age- and sex-matched controls were included. We examined relative EEG power during non-rapid eye movement (NREM) sleep and rapid eye movement (REM) sleep by fast Fourier transform. The Attention Network Test (ANT) was performed to evaluate attention levels. Results: Compared to controls, patients with MDD had lower theta power during NREM (P = 0.018) and REM (P = 0.002) sleep, while higher beta power (P = 0.050) during NREM sleep and delta power (P = 0.018) during REM sleep. Regarding attention level, patients with MDD had lower levels of accuracy (P = 0.021), longer mean reaction time (P < 0.001), poorer manifestations of the alerting effect (P = 0.038) and worse executive control (P = 0.048). Moreover, decreased theta power during NREM sleep was correlated with worsened accuracy (ß = 0.329, P = 0.040), decreased theta power during REM sleep was correlated with worsened alerting effect (ß = 0.355, P = 0.020), and increased delta power during REM sleep was correlated with longer mean reaction time (ß = 0.325, P = 0.022) in patients with MDD. No association between ANT performance and other frequency bands was observed in patients with MDD. Conclusion: Our findings suggest that patients with MDD manifest impaired selective attention function that is associated with decreased theta power during NREM/REM sleep and increased delta power during REM sleep.

Underwater power compensated white light source based on synthetic white laser.

The semiconductor white laser light source is used as a light source for underwater illumination. The required standard color temperature of white light is obtained at the underwater target surface. We studied the power compensation of a synthetic white laser source and its application to underwater illumination. First, the power ratios of the red (638 nm), green (520 nm), and blue (450 nm) lasers at a color temperature of 6500 K were obtained by using chromaticity theory. Next, the three-color and synthetic white laser parameters were obtained with transmission distance, according to the exponential attenuation characteristics of different light in clear water and seawater medium. The three-color laser power at the output was compensated, and the underwater target illumination surface reached the standard 6500 K color temperature of the white laser, improving the illumination. Finally, an experimental system for underwater white laser illumination based on power compensation was established. The errors between experimental and theoretical results of color temperature and illuminance are no more than 0.43% and 22.15%. This power-compensated synthetic white laser light source has both the advantages of long-range underwater detection and the spectral advantages of LED white light sources. The white laser light source meets specific requirements by compensating for power and optimizing white light characteristics for underwater lighting applications.

[Application of 3D printed nasal vestibular support in the treatment of anterior nostril stenosis].

Objective:To evaluate the efficacy of 3D printed nasal vestibular support on the recovery of nasal ventilation function and nostril shape after nostril stenosis treatment. Methods:Thirty-eight patients with unilateral traumatic nasal vestibular stenosis were selected and treated with 3D printed nasal vestibular support after operation. Subjective evaluation indicators, objective nostril local morphological and structural parameters, and nasal airflow dynamics parameters by numerical simulation were used. To evaluate the nostril morphological and nasal functional recovery after treatment. Results:The subjective nasal congestion and nostril symmetry satisfaction VAS scores of the patients after nasal vestibular support treatment were improved to varying degrees compared with those before surgery; The nostril morphological parameters showed that the Δlong-axis ratio and Δ î€Œshort-axis ratio were significantly decreased after nasal vestibular support therapy （0.09±0.09 and 0.16±0.13） compared with those before surgery（0.21±0.20 and 0.28±0.21） respectively（P<0.01）. And the cross-sectional area of the nasal valve on the stenotic side nasal cavity increased from（0.40±0.27） cm² before operation to （0.71±0.26） cm² after treatment（P<0.01）; The nasal resistance on the stenosis side nasal cavity also decreased from （0.036±0.024） Pa·s/mL before operation to （0.022±0.008） Pa. s/mL after treatment（P<0.01）, and the total nasal resistance was decreased from （0.033±0.02） Pas/mL before operation to （0.021±0.007）Pa. s/mL after treatment（P<0.01） ; It also showed that NWE（nasal warming efficiency） and NHE（nasal humidification efficiency） on the stenotic side nasal cavity were significantly decreased after nasal vestibular support therapy（[95.92±2.8]% and [94.55±4.17]%） compared with those before surgery （[97.94±1.97 ]% and [96.19±2.94]%） respectively（P<0.01）. Conclusion:The 3D printed nasal vestibular support for postoperative support treatment on patients with anterior nostril stenosis can reflect the advantages of personalized treatment and allow patients to obtain satisfactory results, and the use of individually designed 3D printed nasal vestibular support can make the shape of anterior nostrils and nasal cavity normal ventilation function recover well, its clinical application prospect is worth looking forward to.

Investigation on the structure of nasal cavity and its airflow field in Crouzon syndrome.

OBJECTIVE: Setup computational fluid dynamics (CFD) model of the nasal cavity in patients with Crouzon syndrome analyze inspiratory airflow hydrokinetics of its nasal cavity. After changing the morphosis structure of the nasal cavity by operation, compare the preoperative and postoperative alteration of the airflow field of the nasal cavity and evaluate the effect of operation on the physiological function of nasal ventilation. METHODS: Eleven patients with Crouzon syndrome were underwent spiral computed tomographic laminar scanning to obtain DICOM data and establish the CFD model. The field features of the nasal cavity with inspiratory static state phase were simulated and analyzed by the Fluent software. The changed data on preoperative and postoperative flow field in the nasal cavity in 5 of 11 patients were compared and analyzed. RESULTS: The nasal cavity of a patient with Crouzon syndrome reflected the structural features of relatively short and high-vaulted anteroposterior diameter. The nasal valve was the narrowest region in the nasal cavity and was the key region of producing obvious pressure drop. The inspiratory static state phase reflected comparatively high local airflow rate (approximately 2.469 m/s) and sheer force of the nasal wall. With the distance increasing from the anterior naris, the pressure inside the nasal cavity was decreased gradually. The pressure drop in the nasal cavity before the front end of the concha nasalis inferior (approximately 2 cm from anterior naris) accounted for most of the pressure of the whole nasal cavity (69%-88% of the overall pressure in nasal cavity and 79.24% on average). Osteotomy advancement and distraction osteogenesis increased the anteroposterior diameter of the nasal cavity and the changed nasal resistance. CONCLUSIONS: By analyzing the structure of the nasal cavity of patients with Crouzon syndrome and the CFD numerical simulation of patients after the procedure, airflow distribution in patients' nasal cavity and the effect of the surgery on the structure of the nasal cavity and airflow field were realized. Nasal valve played a pivotal role in the airflow field distribution of the nasal cavity. Operation changed the nasal resistance, improved the ventilation of nasal cavity, but did not affect the airflow field distribution of nasal cavity.

Exosomal lncRNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)contributes to the progression of allergic rhinitis via modulating microRNA-511/Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2) axis.

Allergic rhinitis (AR) is a common chronic disease characterized by inflammation of the nasal mucosa. Long non-coding RNA (LncRNA) has been reported to be involved in the pathogenesis of various diseases. However, the biological roles of lncRNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) in AR are still unclear. The mRNA levels of NEAT1, miR-511, and Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2) were detected by RT-qPCR. The protein levels of exosomal markers were examined by western blot. ELISA was used to assess the levels of GM-CSF, eotaxin-1, and MUC5AC. The cell viability and apoptosis were evaluated by CCK-8 and TUNEL assays. In this study, we found that the NEAT1 level was highly expressed in AR and IL-13-treated HNECs. NEAT1 interference significantly suppressed levels of GM-CSF, eotaxin-1, and MUC5AC and apoptosis rate, but promoted the viability of IL-13-treated human nasal epithelial cells (HNECs). Moreover, exosomes containing NEAT1 induced inflammatory cytokine production and apoptosis, while NEAT1 depletion abrogated these effects. In addition, NEAT1 directly interacted with miR-511, and the inhibition of miR-511 partially restored the inhibitory effects of NEAT1 silencing on inflammatory cytokine, mucus production, and apoptosis in IL-13-stimulated HNECs. Furthermore, miR-511 could bind to the 3'UTR of NR4A2, and the inhibition of miR-511 increased levels of inflammatory factors and apoptosis rate, which was counteracted by depleting NR4A2. In conclusion, our data revealed that exosomal NEAT1 contributed to the pathogenesis of AR through the miR-511/NR4A2 axis. These findings might offer novel strategies for the prevention and treatment of AR.