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1.
BMC Genet ; 14: 113, 2013 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-24289265

RESUMEN

BACKGROUND: Orange-spotted grouper, Epinephelus coioides, is one of the most valuable fish species in China. Commercial production of orange-spotted grouper could be increased by developing higher growth rates and improving commercially important traits. Information on genetic markers associated with quantitative trait loci (QTL) can be used in breeding programs to identify and select individuals carrying desired traits. A high-density genetic linkage map is the basis for QTL study, and multiplexed shotgun genotyping (MSG) facilitates the development of single nucleotide polymorphisms (SNPs) and genotyping. In this study, the first high-density genetic linkage maps for groupers were generated on the basis of the MSG method. RESULTS: The sex-averaged map contained a total of 4,608 SNPs, which spanned 1581.7 cM, with a mean distance between SNPs of 0.34 cM. The 4,608 SNPs were located in 2,849 unique locations on the linkage map, with an average inter-location space at 0.56 cM. There were 2,516 SNPs on the female map, and the number of unique locus was 1,902. However, the male map contained more numbers of SNP (2,939) and unique locations (2,005). The total length of the female and male maps was 1,370.9 and 1,335.5 cM, respectively. CONCLUSIONS: The high-resolution genetic linkage maps will be very useful for QTL analyses and marker-assisted selection (MAS) for economically important traits in molecular breeding of the orange-spotted grouper.


Asunto(s)
Mapeo Cromosómico , Ligamiento Genético , Genoma/genética , Perciformes/genética , Animales , Femenino , Genotipo , Secuenciación de Nucleótidos de Alto Rendimiento , Masculino , Polimorfismo de Nucleótido Simple , Sitios de Carácter Cuantitativo
2.
Nat Biotechnol ; 30(6): 549-54, 2012 May 13.
Artículo en Inglés | MEDLINE | ID: mdl-22580950

RESUMEN

Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chromosomes and annotated 38,801 genes. Key chromosome reshuffling events were detected through collinearity identification between foxtail millet, rice and sorghum including two reshuffling events fusing rice chromosomes 7 and 9, 3 and 10 to foxtail millet chromosomes 2 and 9, respectively, that occurred after the divergence of foxtail millet and rice, and a single reshuffling event fusing rice chromosome 5 and 12 to foxtail millet chromosome 3 that occurred after the divergence of millet and sorghum. Rearrangements in the C(4) photosynthesis pathway were also identified.


Asunto(s)
Biocombustibles , Evolución Molecular , Genoma de Planta , Setaria (Planta)/genética , Mapeo Cromosómico , Ciclohexanonas/farmacología , Bases de Datos Genéticas , Resistencia a Medicamentos , Marcadores Genéticos/genética , Familia de Multigenes , Fotosíntesis/genética , Filogenia , Análisis de Secuencia de ADN
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