RESUMEN
Sexual differentiation in Drosophila is controlled by a short cascade of regulatory genes, the expression pattern of which determines all aspects of maleness and femaleness, including complex behaviours displayed by males and females [1-3]. One sex-determining gene is transformer (tra), the activity of which is needed for female development. Flies with a female karyotype (XX) but which are mutant for tra develop and behave as males. In such flies, a female phenotype can be restored by a transgene that carries the female-specific cDNA of tra under the control of a heat-shock promoter. This transgene, called hs[trafem], also transforms XY animals into sterile females [4]. When we raised these XX and XY 'females' at 25 degreesC, however, they displayed vigorous male courtship while at the same time, as a result of their female pheromone pattern, they were attractive to males. Intriguingly, their male courtship behaviour was indiscriminately directed towards both females and males. When we forced expression of tra by heat shock, applied during a limited period around puparium formation, male behaviour was abolished and replaced by female behaviour. We conclude that sexual behaviour is irreversibly programmed during a critical period as a result of the activity or inactivity of a single control gene.
Asunto(s)
Drosophila melanogaster/fisiología , Conducta Sexual Animal , Animales , Drosophila melanogaster/genética , Femenino , Genes de Insecto , Infertilidad Femenina/genética , Masculino , Modelos Biológicos , Fenotipo , Caracteres Sexuales , Cromosoma X , Cromosoma YRESUMEN
In Saccharomyces cerevisiae, pseudouridine formation in the middle position of the tRNA(Tyr) anticodon (psi 35) is dependent on the presence of the intron in the tRNA(Tyr) gene (Johnson and Abelson, Nature 302:681-687, 1983). Drosophila melanogaster tRNA(Tyr) genes contain introns of three size classes: 20 or 21 base pairs (bp) (six genes), 48 bp (one gene), and 113 bp (one gene). As in yeast, removal of the intron led to loss of psi 35 in the anticodon when transcription was assayed in Xenopus laevis oocytes. All Drosophila intron sizes supported psi 35 formation. The same results were obtained with the homologous X. laevis tRNA(Tyr) genes containing introns of 12 or 13 bp or with a deleted intron. The introns of yeast (Nishikura and DeRobertis, J. Mol. Biol. 145:405-420, 1981), D. melanogaster, and X. laevis tRNA(Tyr) wild-type genes, while they all supported psi 35 synthesis, did not share any consensus sequences. As discussed, these results, taken together, suggest that for appropriate function the psi 35 enzyme in the X. laevis oocyte needs the presence of an unqualified intron in the tRNA gene and a tRNA(Tyr)-like structure in the unprocessed tRNA precursor.
Asunto(s)
Anticodón , Genes , Intrones , Seudouridina , ARN de Transferencia Aminoácido-Específico/genética , ARN de Transferencia de Tirosina/genética , ARN de Transferencia , Uridina , Animales , Secuencia de Bases , Drosophila melanogaster/genética , Femenino , Datos de Secuencia Molecular , Mutación , Oocitos/enzimología , Uridina/análogos & derivados , Xenopus laevisRESUMEN
A peptide homologous to the Drosophila melanogaster sex-peptide (SP) was isolated from Drosophila suzukii accessory glands and its amino acid sequence determined. The D. suzukii peptide contains 41 amino acids and has a calculated molecular weight of 5100 Da. Comparison of the sequences reveals strong homologies in the N-terminal and C-terminal parts of the peptides. In the D. suzukii sex-peptide, however, five additional amino acids are inserted after amino acid 7. Based on the sequence of the peptide, a cDNA coding for the D. suzukii peptide was isolated by PCR. Sequence analysis of the cDNA confirmed the SP amino acid sequence determined by peptide sequencing. Furthermore, based on the cDNA sequence, we isolated the D. suzukii sex-peptide gene by inverse PCR. The D. suzukii sex-peptide gene contains an intron and codes for a 60 amino acid precursor. The D. melanogaster and the D. suzuki sex-peptides elicit rejection behaviour in the presence of males and an increased egg laying in virgin females of both species.
Asunto(s)
Proteínas de Drosophila , Drosophila melanogaster/genética , Drosophila/genética , Hormonas de Insectos/genética , Péptidos/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Evolución Biológica , Cromatografía Líquida de Alta Presión , ADN , Femenino , Hormonas de Insectos/fisiología , Péptidos y Proteínas de Señalización Intercelular , Intrones , Datos de Secuencia Molecular , Ovulación , Péptidos/fisiología , Reacción en Cadena de la Polimerasa , Precursores de Proteínas/genética , Especificidad de la Especie , Relación Estructura-ActividadRESUMEN
Sex peptide (SP) and Ductus ejaculatorius peptide (Dup) 99B are synthesized in the retrogonadal complex of adult male Drosophila melanogaster, and are transferred in the male seminal fluid to the female genital tract during mating. They have been sequenced and shown to exhibit a high degree of homology in the C-terminal region. Both affect subsequent mating and oviposition by female D. melanogaster. SP also increases in vitro juvenile hormone (JH) biosynthesis in excised corpora allata (CA) of D. melanogaster and Helicoverpa armigera. We herein report that the partial C-terminal peptides SP(8-36) and SP(21-36) of D. melanogaster, and the truncated N-terminal SP(6-20) do not stimulate JH biosynthesis in vitro in CA of both species. Both of these C-terminal peptides reduce JH-III biosynthesis significantly. Dup99B, with no appreciable homology to SP in the N-terminal region, similarly lacks an effect on JH production by H. armigera CA. In contrast, the N-terminal peptides - SP(1-11) and SP(1-22) - do significantly activate JH biosynthesis of both species in vitro. We conclude that the first five N-terminal amino acid residues at the least, are essential for allatal stimulation in these disparate insect species. We have previously shown that the full-length SP(1-36) depresses pheromone biosynthesis in H. armigera in vivo and in vitro. We now show that full-length Dup99B and the C-terminal partial sequence SP(8-36) at low concentrations strongly depress (in the range of 90% inhibition) PBAN-stimulated pheromone biosynthesis of H. armigera. In addition, the N-terminal peptide SP(1-22), the shorter N-terminal peptide SP(1-11) and the truncated N-terminal SP(6-20) strongly inhibit pheromone biosynthesis at higher concentrations.
Asunto(s)
Drosophila melanogaster/fisiología , Hormonas Esteroides Gonadales/fisiología , Mariposas Nocturnas/fisiología , Péptidos/fisiología , Secuencia de Aminoácidos , Animales , Femenino , Hormonas Esteroides Gonadales/biosíntesis , Hormonas Esteroides Gonadales/química , Hormonas Juveniles/biosíntesis , Masculino , Datos de Secuencia Molecular , Péptidos/química , Reproducción/fisiologíaRESUMEN
Male insect accessory glands contain factors that are transferred during mating to the female, some inducing post-mating behavior, including the cessation of pheromone production, non-receptivity and the initiation of oviposition. One such factor is the Drosophila melanogaster sex-peptide (DrmSP). A pheromone suppression peptide, termed HezPSP, was identified in the moth Helicoverpa zea, isolated by HPLC and the active peak sequenced, but the activity of the synthesized peptide has not been reported to date. HezPSP bears no sequence homology to DrmSP. However, both peptides contain a disulfide bridge separated by an equal number, but dissimilar, amino acids. We herein report on the pheromonostatic activity of HezPSP partial peptides in the moth Helicoverpa armigera.
Asunto(s)
Proteínas de Insectos/farmacología , Mariposas Nocturnas/efectos de los fármacos , Mariposas Nocturnas/fisiología , Fragmentos de Péptidos/farmacología , Atractivos Sexuales/antagonistas & inhibidores , Atractivos Sexuales/biosíntesis , Conducta Sexual Animal/efectos de los fármacos , Secuencia de Aminoácidos , Animales , Copulación/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , Proteínas de Insectos/química , Masculino , Datos de Secuencia Molecular , Fragmentos de Péptidos/química , Alineación de Secuencia , Atractivos Sexuales/química , Atractivos Sexuales/farmacologíaRESUMEN
The nucleotide sequence of the two major Drosophila melanogaster tRNATyr isoacceptors was determined to be pC-C-U-U-C-G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3 U-A-G-A-G-C-m2(2)G-G-psi-G-G-A-C-U-G/Q-psi-A-m1G-A-Um-C-C-A-U-A-G-m7 G-D-C-G-C-U-G-G-U(T)-psi-C-A-m1A-A-U-C-C-G-G-C-U-C-G-A-A-G-G-A-A-C-C-AOH . The two isoacceptors differ by the presence of a G or a Q in the wobble position. Both contain a partial modification in position 54 (U/T). Thus, these tRNAs are transcribed from a single gene (or many genes with identical sequences). A fast and sensitive postlabeling method for sequencing tRNA anticodons is described. Nuclease S-1-treated tRNA is labeled with 5[32P]-pCp using T-4 RNA ligase. The tRNA fragments are then separated on 7 M urea/20% PAA gels. After autoradiography the RNA is eluted and digested with T-2 RNase. The nature of the labeled nucleotides is determined by two-dimensional thin-layer chromatography. The same method can be used to determine the 5' sequence of a tRNA by 3' labeling 5' tRNA halves with 5[32P]-pCp and subsequent chemical sequencing.
Asunto(s)
Drosophila melanogaster/genética , Aminoacil-ARN de Transferencia/análisis , Animales , Anticodón , Autorradiografía , Secuencia de Bases , Cromatografía en Capa Delgada , Endonucleasas , Endonucleasas Específicas del ADN y ARN con un Solo FilamentoRESUMEN
Bovine herpesvirus 1 (BHV-1) specifies and unspliced early 2.6-kb RNA (ER2.6) which is 3' coterminal with exon 2 of the 2.9-kb immediate-early (IE) RNA. The two transcripts have a common open reading frame (676 codons). The predicted protein, designated BHV-1 infected cell protein 0 (BICP0), contains a zinc finger domain with homology to ICP0 of herpes simplex virus type 1 and protein 61 of varicella-zoster virus, and depending on the promoter, it acts as a strong activator or as a repressor in transient expression assays. In situ immunoadsorbent assays using antisera against synthetic oligopeptides demonstrated that BICP0 accumulates in nuclei of BHV-1-infected cells, as expected for an IE gene product involved in gene regulation. Western blots (immunoblots) revealed a BHV-1-specific 97-kDa protein which was detectable during the IE phase and also at later periods of infection, indicating that the kinetics of BICP0 synthesis is consistent with the switch from IER2.9 to ER2.6. To confirm that ER2.6 encoded the 97-kDa BICP0 protein, a DNA fragment containing BICP0-coding sequences was inserted into the Autographa californica baculovirus genome. A recombinant protein, identified by its reactivity with antipeptide sera, exhibited the same electrophoretic mobility as BICP0 specified by BHV-1. We microinjected Xenopus oocytes with a BICP0 effector plasmid and a promoter-chloramphenicol acetyltransferase plasmid. BICP0-induced stimulation of this promoter was strongly reduced when intracellular zinc was chelated by thionein, indicating that the effect of BICP0 is zinc dependent.
Asunto(s)
Herpesvirus Bovino 1/metabolismo , Transactivadores/efectos de los fármacos , Activación Transcripcional , Proteínas Virales/efectos de los fármacos , Zinc/farmacología , Secuencia de Aminoácidos , Animales , Anticuerpos Antivirales , Baculoviridae/genética , Bovinos , Compartimento Celular , Núcleo Celular/metabolismo , Células Cultivadas , Ergotioneína/farmacología , Datos de Secuencia Molecular , Mariposas Nocturnas , Oocitos/efectos de los fármacos , Oocitos/metabolismo , Proteínas Recombinantes/efectos de los fármacos , Proteínas Recombinantes/inmunología , Transactivadores/genética , Transactivadores/inmunología , Ubiquitina-Proteína Ligasas , Proteínas Virales/genética , Proteínas Virales/inmunología , Xenopus/metabolismoRESUMEN
After mating, Drosophila melanogaster females lay substantially more eggs and mate rarely. Central to these changes is Sex peptide (SP), a male peptide transferred into the female during copulation. Injected into virgins, SP induces the same post mating response as observed after mating. In this study we investigated the role of the mushroom body (MB) in the SP response system. The SP response of females with either chemically ablated or mutant MBs was analyzed. After injection of SP, females with chemically ablated MBs reduce their receptivity and increase their ovulation and oviposition to the level of females with intact MBs. Virgin females with ablated MBs, however, show a constitutively elevated oviposition rate. Hence in untreated females, MBs are not implicated in the SP-induced reduction of receptivity and increase of ovulation. However, they depress the oviposition rate of virgins. Thus, SP has two functions for oviposition: it de-represses the MB-dependent block on the egg laying activity of virgins and additionally stimulates oviposition. SP-injected mushroom body miniature (mbm) females lay fewer eggs, ovulate less frequently, and mate more often than wild-type females. A model of the putative role of MBs and the gene product of mbm in SP-induced oviposition is presented.