Soilless cultivation systems to produce tailored microgreens for specific nutritional needs.

BACKGROUND: The awareness of the importance of following dietary recommendations that meet specific biological requirements related to an individual's health status has significantly increased interest in personalized nutrition. The aim of this research was to test agronomic protocols based on soilless cultivation for providing consumers with new dietary sources of iodine (I), as well as alternative vegetable products to limit dietary potassium (K) intake; proposed cultivation techniques were evaluated according to their suitability to obtain such products without compromising agronomic performance. RESULTS: Two independent experiments, focused on I and K respectively, were conducted in a commercial greenhouse specializing in soilless production. Four different species were cultivated using three distinct concentrations of I (0, 1.5 and 3 mg L-1 ) and K (0, 60 and 120 mg L-1 ). Microgreens grown in I-rich nutrient solution accumulate more I, and the increase is dose-dependent. Compared to unbiofortified microgreens, the treatments with 1.5 and 3 mg L-1 of I resulted in 4.5 and 14 times higher I levels, respectively. Swiss chard has the highest levels of K (14 096 mg kg-1 of FW), followed by rocket, pea and radish. In radish, rocket and Swiss chard, a total reduction of K content in the nutrient solution (0 mg L-1 ) resulted in an average reduction of 45% in K content. CONCLUSION: It is possible to produce I-biofortified microgreens to address I deficiency, and K-reduced microgreens for chronic kidney disease-affected people. Species selection is crucial to customize nutritional profiles according to specific dietary requirements due to substantial mineral content variations across different species. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Characterization of Micronutrients, Bioaccessibility and Antioxidant Activity of Prickly Pear Cladodes as Functional Ingredient.

The Opuntia ficus indica (L.) (OFI) is used as a nutritional and pharmaceutical agent in various dietary and value added products. This study underlines the possible use of native prickly pear cladode powder as a functional ingredient for health-promoting food production. To summarise, chemical characterization of polyphenols, minerals and soluble dietary fibre was performed; furthermore, the antioxidant activity and bioaccessibility of polyphenols and minerals were assessed. Eleven compounds between phenolic acids and flavonoids were identified, with piscidic acid and isorhamnetin derivatives being the most abundant. Opuntia's dietary fibre was mainly constituted of mucilage and pectin, and was composed of arabinose, galactose, glucose, mannose, rhamnose, and xylose sugars. The polyphenols' bioaccessibility was very high: piscidic acid at 200%, eucomic and ferulic acids >110% and flavonoids from 89% to 100%. The prickly pear cladode powder is also a source of minerals, as cations (calcium, sodium, potassium and magnesium) and anions (sulphate and chloride), with high magnesium bioaccessibilty (93%). OFI powder showed good capacity of radical scavenging measured by DPPH and ABTS methods, with 740 and 775 µmol Trolox/100 g OFI, respectively. Finally, the presented results allow the consideration of this natural product as a source of several essential nutrients, with a possible use in the food industry as a functional ingredient.

Certification of nitrate in spinach powder reference material SPIN-1 by high-precision isotope dilution GC-MS.

A high-precision exact-matching quadruple isotope dilution method (ID4MS) was employed for the quantitation of nitrate in an air-dried spinach powder Certified Reference Material (CRM). The analyte was extracted in hot water following addition of 15NO[Formula: see text] internal standard. The blend was then treated with sulfamic acid to remove nitrite and with triethyloxonium tetrafluoroborate to promote aqueous conversion of nitrate into volatile EtONO2. The derivative was analyzed by headspace GC-MS with 3-min elution time. The method performance was validated with a series of tests which demonstrated adequate selectivity and ruggedness. This method supported the development of novel SPIN-1 CRM giving a modest contribution to its uncertainty (uchar = 0.85%). With respect to previous attempts, the SPIN-1 was proven stable, homogeneous (uhom = 0.44%), and suitable for spinach monitoring under EU regulations. On dried basis, the nitrate content of SPIN-1 was found to be 22.53 ± 0.43 mg/g (Uc = 1.9%, k = 2). The material was also used in an inter-laboratory study where four laboratories employed a total of ten measurement methods. Graphical Abstract SPIN-1 Certified Reference Material for nitrate in spinach powder.

Silicon biofortification of leafy vegetables and its bioaccessibility in the edible parts.

BACKGROUND: The mineral silicon (Si) is an essential element for humans and a general component of the diet found mainly in plant-based foods. The aim of this study was to obtain Si biofortificated leafy vegetables (tatsoi, mizuna, purslane, basil, Swiss chard, and chicory) to use for the fresh-cut products (ready to use). For the production of biofortified plants, a floating system with 0, 50 and 100 mg L(-1) of Si in nutrient solution, was used. In addition, the assessment of bioaccessibility of biofortified plants, by in vitro gastro-digestion process, was performed. RESULTS: The added silicon in nutrient solution did not influence yield and colour of vegetables but a species-related accumulation of Si (expressed as SiO2) was found: from 18 to 69 mg kg(-1) fresh weight (FW) in tatsoi, from 19 to 106 mg kg(-1) FW in mizuna, from 15 to 93 mg kg(-1) FW in purslane, from 41 to 294 mg kg(-1) FW in basil, from 17 to 76 mg kg(-1) FW in Swiss chard, and from 23 to 76 mg kg(-1) FW in chicory. The Si became bioaccessible in all species considered in a range from 23% (basil) to 64% (chicory). CONCLUSION: The application of Si to the nutrient solution in the range of 50-100 mg L(-1) allows biofortification of leafy vegetables. In addition, the biofortified vegetables showed, on average, more bioaccessible Si, with respect to unbiofortified vegetables.

Quality and Safety of Ready-to-Eat Golden Thistle (Scolymus hispanicus L.): A New Product for Traditional Italian Dishes.

Golden thistle (Scolymus hispanicus L.) is a wild edible plant belonging to Asteraceae family, with a great potential for food applications. The aim of this study was to identify the best cooking procedure able to provide a high-quality, ready-to-use product. For this purpose, leaf midribs (the most used edible part of the plant) were cooked by boiling, steaming, and 'sous vide', and the cooked products were compared for their phenolic content and composition, antioxidant activity, sugar and inorganic ion content, organoleptic characteristics, and microbial safety, this latter also during storage. In general, boiling caused a decrease in the value of these parameters, despite being the best product for taste and overall acceptability. On the contrary, steaming and 'sous vide' resulted in the best treatments to preserve antioxidant activity, total phenols, and chlorogenic acid. In particular, in 'sous vide' cooked samples, a significant increase in the value of these parameters and a remarkable decrease in nitrate content were found. Moreover, 'sous vide' resulted in the best treatment also regarding microbial safety during shelf life; actually, after 15 days of storage at 8 °C, Enterobacteriaceae and mesophilic aerobic bacteria were not detectable in 'sous vide' samples. These results contributed to increase the knowledge of a wild edible plant with high nutritional properties and promoting its consumption by obtaining a ready-to-use product with good organoleptic characteristics and endowed with a long period of shelf life.

Soilless biofortification, bioaccessibility, and bioavailability: Signposts on the path to personalized nutrition.

Propelled by an ever-growing awareness about the importance of following dietary recommendations meeting specific biological requirements linked to a person health status, interest in personalized nutrition is on the rise. Soilless biofortification of vegetables has opened the door to the potential for adapting vegetable production to specific dietary requirements. The evolution of vegetables biofortification toward tailored food is examined focusing on some specific categories of people in a context of personalized nutrition instead to simple describe developments in vegetables biofortification with reference to the single element or compound not adequately present in the daily diet. The concepts of bioavailability and bioaccessibility as a useful support tool for the precision biofortification were detailed. Key prospects for challenges ahead aiming to combine product quality and sustainable are also highlighted. Hydroponically cultivation of vegetables with low potassium content may be effective to obtain tailored leafy and fruit vegetable products for people with impaired kidney function. Simultaneous biofortification of calcium, silicon, and boron in the same vegetable to obtain vegetable products useful for bone health deserve further attention. The right dosage of the lithium in the nutrient solution appears essential to obtain tailored vegetables able to positively influence mental health in groups of people susceptible to mental illness. Modulate nitrogen fertilization may reduce or enhance nitrate in vegetables to obtain tailored products, respectively, for children and athletes. Future research are needed to produce nickel-free vegetable products for individuals sensitized to nickel. The multidisciplinary approach toward tailored foods is a winning one and must increasingly include a synergy between agronomic, biological, and medical skills.

Nutraceutical Profile of "Carosello" (Cucumis melo L.) Grown in an Out-of-Season Cycle under LEDs.

The world population is projected to increase to 9.9 billion by 2050 and, to ensure food security and quality, agriculture must sustainably multiply production, increase the nutritional value of fruit and vegetables, and preserve genetic variability. In this work, an Apulian landrace of Cucumis melo L. called "Carosello leccese" was grown in a greenhouse with a soilless technique under light-emitting diodes (LEDs) used as supplementary light system. The obtained results showed that "Carosello leccese" contains up to 71.0 mg·g-1 dried weight (DW) of potassium and several bioactive compounds important for human health such as methyl gallate (35.58 µg·g-1 DW), α-tocopherol (10.12 µg·g-1 DW), and ß-carotene (up to 9.29 µg·g-1 DW under LEDs). In fact, methyl gallate has antioxidative and antiviral effects in vitro and in vivo, tocopherols are well recognized for their effective inhibition of lipid oxidation in foods and biological systems and carotenoids are known to be very efficient physical and chemical quenchers of singlet oxygen. Finally, it was demonstrated that the LEDs' supplementary light did not negatively influence the biochemical profile of the peponids, confirming that it can be considered a valid technique to enhance horticultural production without reducing the content of the bioactive compounds of the fruits.

Mineral Composition and Bioaccessibility in Rocket and Purslane after Zn Biofortification Process.

Zinc (Zn) is an essential key nutrient in different biochemical and physiological processes. The nutritional deficit of this mineral element is estimated to affect the health of over 3 billion people worldwide. Several strategies are available to reduce the negative impact of mineral malnutrition; among them, biofortification is the practice of deliberately increasing the nutrients and healthy compounds in the edible parts of vegetables. This study aims to evaluate Zn bioaccessibility in biofortified and non-biofortified rocket and purslane using an in vitro gastrointestinal digestion process and measure the concentration of other mineral elements (Al, B, Ca, Fe, K, Mg, Mn, and Sr) released during the digestion process from rocket and purslane biofortified with Zn. The bioaccessible Zn in biofortified rocket and purslane ranged from 7.43 to 16.91 mg/kg, respectively. In addition, the daily intake, the RDA coverage (%), and the hazard quotient (HQ) for the intake of Zn (resulting from the consumption of 100 g of rocket and purslane) were calculated. The calculated HQ highlights the safety of these baby leaf vegetables. The study confirms that it is possible to obtain Zn-biofortified rocket and purslane with high Zn bioaccessibility by adopting an appropriate mineral plant nutrition solution enriched in Zn.

Enhancing the nutritional value of Portulaca oleracea L. by using soilless agronomic biofortification with zinc.

Zinc (Zn) plays a crucial role for human health. Zn deficiency is a common problem worldwide, causing health problems specifically related with detrimental effects on immune system. In this study we used hydroponic floating system and nutrient solutions (NS) with different levels of Zn (0.13 - control, 1.3, 2.6 and 5.2 mg/L Zn) in order to test a biofortification process aimed to increase the Zn tissue content of two different varieties (commercial and wild) of purslane. We evaluated the effects of the treatments on yield, visual and overall nutritional quality of the edible part of plants. Biofortification treatments did not affect plant yield, but increased the Zn content in the edible part of purslane by 1.8, 2.3 and 2.7-fold, respectively with 1.3, 2.6 and 5.2 mg/L Zn in the NS, so that the consumption of a serving portion of 150 g of baby leaf purslane biofortified with the highest Zn concentration would account for 21% of RDA (recommended daily allowance) for this nutrient. The Zn biofortification process did not affect colour parameters, therefore no differences in the product visual quality were observed. Zn treatment with 5.2 mg/L allowed to obtain an increase in neoxanthin, lutein and ß-carotene, while it was not possible to outline a common trend for the fatty acids profile in relation to the Zn treatments. The consumption of hydroponic purslane biofortified with Zn may allow to improve the Zn nutritional status of consumers, and provides different important phytochemicals, such as carotenoids and unsaturated fatty acids.

Posidonia Natural Residues as Growing Substrate Component: An Ecofriendly Method to Improve Nutritional Profile of Brassica Microgreens.

The aim of this study was to test Posidonia oceanica (L.) Delile seagrass residues (leaves and fibers) as growing media component to improve the nutritional quality of two different brassica microgreens (Mizuna and Rapini). We hypothesized that addition of posidonia residues in the substrate would result in higher concentration of certain mineral nutrients in the edible parts of plants. Substrates were obtained by mixing leaves and fibers, each material at the rate of 25, 50 and 75% (v/v), with a peat based commercial substrate, that was also used at 100% rate as a control treatment. Two experiments were carried out (Experiment 1: Mizuna microgreens production in growth chamber conditions; Experiment 2: Mizuna and Rapini microgreens production in greenhouse conditions). Plant growth measurements and chemical analysis on edible parts (mineral tissue composition and main bioactive compounds - polyphenol, chlorophylls and carotenoids contents) were performed in order to evaluate the effects of the different substrates on growth and nutritional composition of brassica microgreens. In order to evaluate the consumer safety, daily intake, percentage of recommended daily allowance for I (RDA-I) and hazard quotient (HQ) for I intake through consumption of 50 and 100 g portions of Rapini microgreens were calculated. Posidonia in the growing media mixtures increased I and B content in edible parts of microgreens. The calculated HQ underlines the safety of these products. Results confirm the possibility to improve nutritional profile of brassica microgreens by using this natural material as a growing media component, resulting in a sustainable approach.

New insight into microbial degradation of mycotoxins during anaerobic digestion.

Anaerobic digestion represents an interesting approach to produce biogas from organic waste materials contaminated by mycotoxins. In this study a shotgun metagenomic analysis of lab-scale bioreactors fed with mycotoxin-contaminated silage has been carried out to characterize the evolution of microbial community under the operating conditions and the key enzymatic activities responsible for mycotoxin degradation. The study was conducted at two different level of contamination for fumonisins and aflatoxin B1. After 15 days biogas production was not influenced by the presence of mycotoxins. Metagenomic analysis revealed that a high contamination rate of mycotoxins interfere with microbial diversity. Degradation of mycotoxins accounted in about 54% for aflatoxin B1 and 60% for fumonisins. The degradation activity of fumonisins resulted in the presence of partially hydrolyzed forms in both tested contamination levels. Accordingly, metagenomic functional analysis revealed the presence of two new carboxylesterase genes belonging to D. bacterium and P. bacterium putatively involved in fumonisin degradation.

Supplementary Light Differently Influences Physico-Chemical Parameters and Antioxidant Compounds of Tomato Fruits Hybrids.

One of the challenges for agriculture in the coming years will be producing more food avoiding reducing the nutritional values of fruits and vegetables, sources of nutraceutical compounds. It has been demonstrated that light-emitting diodes (LEDs) used as a supplementary light (SL) technology improve tomato yield in Mediterranean greenhouses, but few data have been reported about SL effects on fruit physio-chemical parameters. In this study, three tomato hybrid (F1) cultivars were grown for year-round production in a commercial semi-closed glasshouse in Southern Italy: red cherry type ("Sorentyno"), red plum type ("Solarino"), and yellow plum type ("Maggino"). From 120 to 243 days after transplant (DAT), Red/White/Blue LEDs were used as SL. The fruits harvested 180 DAT were analyzed and those obtained under LEDs had 3% more dry weight, 15% more total soluble solids, and 16% higher titratable acidity than fruits grown only under natural light. Generally, the antioxidant activity and the mineral profile of the fruits were not negatively influenced by SL. Lycopene content was unchanged and vitamin C content of "Sorentyno" even increased by 15% under LEDs. Overall, LEDs used as SL technology could be one of the tools used by agriculture in Mediterranean basin to produce more food maintaining high quality production.

Barattiere: An Italian Local Variety of Cucumis melo L. with Quality Traits between Melon and Cucumber.

Barattiere, belonging to the Cucumis melo L. species, is a local variety of Puglia (Southern Italy), which is consumed as a vegetable at the immature stage, like cucumber. In this study, three Barattiere populations ('Monopoli', 'Carovigno' and 'Fasano') were evaluated for the main quality traits. All genotypes showed a very light green-yellow colour of flesh, without any difference regarding chlorophyll and carotenoid contents. Carovigno's Barattiere showed the highest values of dry weight (6.8 g 100 g-1 fresh weight - FW), sugars (45 g kg-1 FW), and sweetness index (7.3), while Monopoli's Barattiere showed the lowest total phenols content (21 mg kg-1 FW). Fasano's Barattiere showed the highest content of Zn and Cu (2.3 and 0.3 mg kg-1 FW, respectively), while 'Monopoli' showed the highest Ba content (0.3 mg kg-1 FW) and the lowest Mg content (94 mg kg-1 FW). No differences between populations were found concerning the content of Ca, K, Na, B, Mn, and Fe. In conclusion, the quality profile of Barattiere makes this local genotype interesting for its traits, and also suggests its consumption by people with specific dietary requirements.

Living Mulch and Organic Fertilization to Improve Weed Management, Yield and Quality of Broccoli Raab in Organic Farming.

Living mulch gives many benefits to agro-ecosystems such as erosion control, nitrogen fixation and nutrient recycling, increasing of organic matter, weed and pest control, and increasing of soil organism. The experiment, carried out in Puglia, Southern Italy on transplanted broccoli raab (cv. Grossa fasanese), evaluated four soil management systems (SMSs): Trifolium subterraneum and T. repens used as living mulch, undisturbed weedy, and conventional tillage. For each SMS, four rates of nitrogen and phosphorous (NP0, NP1, NP2, and NP3) were supplied, using an organic fertilizer. The following data were collected: weed infestation, leaf chlorophyll in the plants (as SPAD units), weight, diameter, and colour of the inflorescences, anion and Mg, Fe, Na, K, Ca content. Fertilization showed prominent effects on most of parameters evaluated. The Sufficient Index of broccoli raab plants was higher in fertilized plots. With the increasing of fertilization rates, weight of primary inflorescences and the marketable yield linearly increased, confirming the great influence of nitrogen fertilization on the yield of Brassicaceae vegetables and highlighting the importance of combining living mulch and fertilization. By increasing fertilization rates, some elements, such as Mg and Fe, increased, whereas a decrease of Na, K, and Ca was observed. The nitrate content in the inflorescences was different only between the fertilized and unfertilized plots, although it was very low. In NP2 and NP3 a greener colour was found. Living mulch did not clearly affect quality and yield of broccoli raab but was effective in weed control. Results show the positive effects of living mulch and organic fertilization in the sustainable production of broccoli raab.

Iodine Biofortification of Four Brassica Genotypes is Effective Already at Low Rates of Potassium Iodate.

The use of iodine-biofortified vegetables may be a health alternative instead of iodine-biofortified salt for preventing iodine (I) deficiency and related human disorders. In this study, four Brassica genotypes (broccoli raab, curly kale, mizuna, red mustard) were hydroponically grown with three I-IO3- rates (0, 0.75 and 1.5 mg/L) to produce iodine-biofortified vegetables. Crop performances and quality traits were analyzed; iodine content was measured on raw, boiled, and steamed vegetables. The highest I rate generally increased I content in all Brassica genotypes, without plants toxicity effects in terms of reduced growth or morphological symptoms. After 21 day-iodine biofortification, the highest I content (49.5 µg/100 g Fresh Weight (FW)) was reached in broccoli raab shoots, while after 43 day-iodine biofortification, genotype differences were flattened and the highest I content (66 µg/100 g FW, on average) was obtained using 1.5 mg I-IO3/L. Nitrate content (ranging from 1800 to 4575 mg/kg FW) was generally higher with 0.75 mg I-IO3/L, although it depended on genotypes. Generally, boiling reduced iodine content, while steaming increased or left it unchanged, depending on genotypes. Applying low levels of I proved to be suitable, since it could contribute to the partial intake of the recommended dose of 150 µg/day: A serving size of 100 g may supply on average 24% of the recommended dose. Cooking method should be chosen in order to preserve and/or enhance the final I amount.

Morphological and Chemical Profile of Three Tomato (Solanum lycopersicum L.) Landraces of A Semi-Arid Mediterranean Environment.

Puglia (Southern Italy), particularly rich in tomato agro-biodiversity, can be considered a typical region of the semi-arid Mediterranean environments. In this study, three local varieties of tomato (Manduria, Giallo di Crispiano and Regina) were characterized by using morphological descriptors according to international standards. Chemical (isoprenoids, ascorbic acid, total phenols, sugars and mineral content) and agronomic assessment were carried out to highlight the specific traits of these local varieties well adapted to a semi-arid environment. Data of morphological traits according to the "International Union for the Protection of New Varieties of Plants" (UPOV) guideline evidenced a clear distinctness among all three landraces, especially as regards fruits. Results also highlighted that a great part of variation in chemical traits was almost exclusively due to genotypes, while in a few cases observed differences resulted from the interaction between genotype and harvest time. The results of the present study may represent the first step toward the recognition of "conservation variety" status for Regina, Giallo di Crispiano and Manduria tomato landraces. At the same time, both quality traits and agronomic performance of these tomato genotypes suggest the possibility of their cultivation in other semi-arid environments also considering their quality traits, in view of a sustainable production.

Preliminary Evidences of Biofortification with Iodine of "Carota di Polignano", An Italian Carrot Landrace.

The "Carota di Polignano" (Polignano Carrot - PC, Daucus carota L.) is a multi-colored landrace, cultivated in the Southern Italy, whose colors range from yellow to purple. Iodine is an essential micronutrient for humans, since it is a key component of thyroid hormones, which regulate the growth and development of the human body. The main source for iodine assumption is represented by diet, but its concentration in the vegetables is usually limited with respect to human needs. To this purpose, two experimental trials (in open field and in greenhouse with a soil-less system) were carried out to enrich PC with iodine. Three levels of iodine (control treatment, C - 0 mg·L-1; low, L - 50 mg·L-1; and high, H - 500 mg·L-1), distributed with foliar spray fertilizations (in both open field and greenhouse) or with nutrient solution (in greenhouse, at the level of 50 mg·L-1) in the form of KIO3 were compared. In open field, the H treatment showed a biofortification that was double and triple respect to L and C treatments, respectively, without influencing color and biometric parameters, such as the fresh and dry weight of roots and DM percentage. In greenhouse, the biofortification done with foliar spray fertilization followed the same trend of open field, while the biofortification by means of nutrient solution was more effective but reached very high levels that had toxic effects on the plants and could be too high for human nutrition. However, the concentrations of iodine into biofortified carrots in open field can allow to satisfy the recommended daily allowance (RDA) by consuming 100 and 200 g of fresh product for the treatment H and L, respectively. Regarding the greenhouse biofortification, the RDA would be satisfied by consuming 200 g of fresh carrots (with the high level of foliar fertilization).

Integrated in vitro approaches to assess the bioaccessibility and bioavailability of silicon-biofortified leafy vegetables and preliminary effects on bone.

Food industries are increasingly oriented toward new foods to improve nutritional status and/or to combat nutritional deficiency diseases. In this context, silicon biofortification could be an innovative tool for obtaining new foods with possible positive effects on bone mineralization. In this paper, an alternative and quick in vitro approach was applied in order to evaluate the potential health-promoting effects of five silicon-biofortified leafy vegetables (tatsoi, mizuna, purslane, Swiss chard and chicory) on bone mineralization compared with a commercial silicon supplement. The silicon bioaccessibility and bioavailability of the five leafy vegetables (biofortified or not) and of the supplement were assessed by applying a protocol consisting of in vitro gastrointestinal digestion coupled with a Caco-2 cell model. Silicon bioaccessibility ranged from 0.89 to 8.18 mg/L and bioavailability ranged from 111 to 206 µg/L of Si for both vegetables and supplement. Furthermore, the bioavailable fractions were tested on a human osteoblast cell model following the expression of type 1 collagen and alkaline phosphatase. The results obtained highlighted that the bioavailable fraction of biofortified purslane and Swiss chard improved the expression of both osteoblast markers compared with the supplement and other vegetables. These results underline the potentially beneficial effect of biofortified leafy vegetables and also indicate the usefulness of in vitro approaches for selecting the best vegetable with positive bone effects for further in vivo research.

Calcium biofortification and bioaccessibility in soilless "baby leaf" vegetable production.

Calcium is an essential nutrient for human health, because it is a structural component and takes part in a variety of biological processes. The aim of this study was to increase Ca content of baby leaf vegetables (BLV: basil, mizuna, tatsoi and endive), as fresh-cut products. For the production of biofortified BLV, a floating system with two level of Ca (100 and 200mgL(-1)) in the nutrient solution was used. In addition, the assessment of bioaccessibility of Ca, by in vitro digestion process, was performed. In all vegetables, the Ca biofortification (200mgL(-1)) caused a significant Ca enrichment (9.5% on average) without affecting vegetables growth, oxalate contents and marketable quality. Calcium bioaccessibility ranged from 25% (basil) to 40% (endive) but the biofortified vegetables showed more bioaccessible Ca. These results underline the possibility to obtain Ca biofortified BLV by using agronomic approaches.