RESUMEN
OBJECTIVE: To develop cryopreservation for Oenococcus oeni. METHODS: We investigated the influence of the age and concentration of the strain in the protective medium, rate of freezing, temperature of thawing and the protective medium on the number of viable cells after cryopreservation by colony counting. RESULTS: We got the highest number of viable cells after cryopreservation by collecting cells in the early stationary growth phase, keeping their concentration in the protective medium at 10(9) CFU/mL, transferring cells into liquid nitrogen directly and thawing in a water bath at 37 degrees C. The ratio of viable cells was above 99% for 21 of the Oenococcus oeni after storing in liquid nitrogen for 6 months. CONCLUSION: We The storage of Oenococcus oeni in liquid nitrogen is convenient for strains preserved in culture collections.