The ability of selected fungal strains to produce carboxylesterase enzymes for biodegradation and use of bifenthrin insecticide as carbon source: in vitro and in silico approaches.

Bifenthrin (BF) is a broad-spectrum type I pyrethroid insecticide that acts on insects by impairing the nervous system and inhibiting ATPase activity, and it has toxic effects on non-target organisms and high persistence in the environment. This study aimed to determine the potential of six different fungi, including Pseudozyma hubeiensis PA, Trichoderma reesei PF, Trichoderma koningiopsis PD, Purpureocillium lilacinum ACE3, Talaromyces pinophilus ACE4, and Aspergillus niger AJ-F3, to degrade BF. Three different concentrations of BF, including 0.1%, 0.2%, and 0.3% w/v, were used in the sensitivity testing that revealed a significant (p ≤ 0.01) impact of BF on fungal growth. Enzymatic assays demonstrated that both intracellular and extracellular carboxylesterases hydrolyzed BF with the enzymatic activity of up to 175 ± 3 U (µmol/min) and 45 ± 1 U, respectively. All tested fungi were capable of utilizing BF as a sole carbon source producing 0.06 ± 0.01 to 0.45 ± 0.01 mg dry biomass per mg BF. Moreover, the presence of PytH was determined in the fungi using bioinformatics tools and was found in A. niger, T. pinophilus, T. reesei, and P. lilacinum. 3D structures of the PytH homologs were predicted using AlphaFold2, and their intermolecular interactions with pyrethroids were determined using MOE. All the homologs interacted with different pyrethroids with a binding energy of lesser than - 10 kcal/mol. Based on the study, it was concluded that the investigated fungi have a greater potential for the biodegradation of BF.

Omics technology draws a comprehensive heavy metal resistance strategy in bacteria.

The rapid industrial revolution significantly increased heavy metal pollution, becoming a major global environmental concern. This pollution is considered as one of the most harmful and toxic threats to all environmental components (air, soil, water, animals, and plants until reaching to human). Therefore, scientists try to find a promising and eco-friendly technique to solve this problem i.e., bacterial bioremediation. Various heavy metal resistance mechanisms were reported. Omics technologies can significantly improve our understanding of heavy metal resistant bacteria and their communities. They are a potent tool for investigating the adaptation processes of microbes in severe conditions. These omics methods provide unique benefits for investigating metabolic alterations, microbial diversity, and mechanisms of resistance of individual strains or communities to harsh conditions. Starting with genome sequencing which provides us with complete and comprehensive insight into the resistance mechanism of heavy metal resistant bacteria. Moreover, genome sequencing facilitates the opportunities to identify specific metal resistance genes, operons, and regulatory elements in the genomes of individual bacteria, understand the genetic mechanisms and variations responsible for heavy metal resistance within and between bacterial species in addition to the transcriptome, proteome that obtain the real expressed genes. Moreover, at the community level, metagenome, meta transcriptome and meta proteome participate in understanding the microbial interactive network potentially novel metabolic pathways, enzymes and gene species can all be found using these methods. This review presents the state of the art and anticipated developments in the use of omics technologies in the investigation of microbes used for heavy metal bioremediation.

Potentiating Biosynthesis of Alkaloids and Polyphenolic Substances in Catharanthus roseus Plant Using ĸ-Carrageenan.

Catharanthus roseus is a medicinal plant that produces indole alkaloids, which are utilized in anticancer therapy. Vinblastine and vincristine, two commercially important antineoplastic alkaloids, are mostly found in the leaves of Catharanthus roseus. ĸ-carrageenan has been proven as plant growth promoting substance for a number of medicinal and agricultural plants. Considering the importance of ĸ-carrageenan as a promoter of plant growth and phytochemical constituents, especially alkaloids production in Catharanthus roseus, an experiment was carried out to explore the effect of ĸ-carrageenan on the plant growth, phytochemicals content, pigments content, and production of antitumor alkaloids in Catharanthus roseus after planting. Foliar application of ĸ-carrageenan (at 0, 400, 600 and 800 ppm) significantly improved the performance of Catharanthus roseus. Phytochemical analysis involved determining the amount of total phenolics (TP), flavonoids (F), free amino acids (FAA), alkaloids (TAC) and pigments contents by spectrophotometer, minerals by ICP, amino acids, phenolic compounds and alkaloids (Vincamine, Catharanthine, Vincracine (Vincristine), and vinblastine) analysis uses HPLC. The results indicated that all examined ĸ-carrageenan treatments led to a significant (p ≤ 0.05) increase in growth parameters compared to the untreated plants. Phytochemical examination indicates that the spray of ĸ-carrageenan at 800 mg L-1 increased the yield of alkaloids (Vincamine, Catharanthine and Vincracine (Vincristine)) by 41.85 µg/g DW, total phenolic compounds by 3948.6 µg gallic/g FW, the content of flavonoids 951.3 µg quercetin /g FW and carotenoids content 32.97 mg/g FW as compared to the control. An amount of 400 ppm ĸ-carrageenan treatment gave the best contents of FAA, Chl a, Chl b and anthocyanin. The element content of K, Ca, Cu, Zn and Se increased by treatments. Amino acids constituents and phenolics compounds contents were altered by ĸ-carrageenan.

Omicron variant genome evolution and phylogenetics.

Following the discovery of the SARS-CoV-2 Omicron variant (B.1.1.529), the global COVID-19 outbreak has resurfaced after appearing to be relentlessly spreading over the past 2 years. This new variant showed marked degree of mutation, compared with the previous SARS-CoV-2 variants. This study investigates the evolutionary links between Omicron variant and recently emerged SARS-CoV-2 variants. The entire genome sequences of SARS-CoV-2 variants were obtained, aligned using Clustal Omega, pairwise comparison was computed, differences, identity percent, gaps, and mutations were noted, and the identity matrix was generated. The phylogenetics of Omicron variants were determined using a variety of evolutionary substitution models. The ultrametric and metric clustering methods, such as UPGMA and neighbor-joining (NJ), using nucleotide substitution models that allowed the inclusion of nucleotide transitions and transversions as Kimura 80 models, revealed that the Omicron variant forms a new monophyletic clade that is distant from other SARS-CoV-2 variants. In contrast, the NJ method using a basic nucleotide substitution model such as Jukes-Cantor revealed a close relationship between the Omicron variant and the recently evolved Alpha variant. Based on the percentage of sequence identity, the closest variants were in the following order: Omicron, Alpha, Gamma, Delta, Beta, Mu, and then the SARS-CoV-2 USA isolate. A genome alignment with other variants indicated the greatest number of gaps in the Omicron variant's genome ranging from 43 to 63 gaps. It is possible, given their close relationship to the Alpha variety, that Omicron has been around for much longer than predicted, even though they created a separate monophyletic group. Sequencing initiatives in a systematic and comprehensive manner is highly recommended to study the evolution and mutations of the virus.

Phytochemical and Potential Properties of Seaweeds and Their Recent Applications: A Review.

Since ancient times, seaweeds have been employed as source of highly bioactive secondary metabolites that could act as key medicinal components. Furthermore, research into the biological activity of certain seaweed compounds has progressed significantly, with an emphasis on their composition and application for human and animal nutrition. Seaweeds have many uses: they are consumed as fodder, and have been used in medicines, cosmetics, energy, fertilizers, and industrial agar and alginate biosynthesis. The beneficial effects of seaweed are mostly due to the presence of minerals, vitamins, phenols, polysaccharides, and sterols, as well as several other bioactive compounds. These compounds seem to have antioxidant, anti-inflammatory, anti-cancer, antimicrobial, and anti-diabetic activities. Recent advances and limitations for seaweed bioactive as a nutraceutical in terms of bioavailability are explored in order to better comprehend their therapeutic development. To further understand the mechanism of action of seaweed chemicals, more research is needed as is an investigation into their potential usage in pharmaceutical companies and other applications, with the ultimate objective of developing sustainable and healthier products. The objective of this review is to collect information about the role of seaweeds on nutritional, pharmacological, industrial, and biochemical applications, as well as their impact on human health.

The Synergistic Hepatoprotective Activity of Rosemary Essential Oil and Curcumin: The Role of the MEK/ERK Pathway.

BACKGROUND: Curcumin is a natural product obtained from the rhizome of Curcuma longa. Rosemary (Rosmarinus officinalis) is a medicinal and aromatic plant that is widely spread in the Mediterranean region. Both Curcumin and rosemary essential oil are natural products of high medicinal and pharmacological significance. The hepatoprotective effect of both natural products is well-established; however, the mechanism of such action is not fully understood. Thus, this study is an attempt to explore the hepatoprotective mechanism of action of these remedies through their effect on MEK and ERK proteins. Furthermore, the effect of rosemary essential oil on the plasma concentration of curcumin has been scrutinized. MATERIALS AND METHODS: The major constituents of REO were qualitatively and quantitatively determined by GC/MS and GC/FID, respectively. Curcumin and rosemary essential oil were given to mice in a pre-treatment model, followed by induction of liver injury through a high dose of paracetamol. Serum liver enzymes, lipid peroxidation, antioxidant activities, the inflammatory and apoptotic biomarkers, as well as the MEK and ERK portions, were verified. The plasma levels of curcumin were determined in the presence and absence of rosemary essential oil. RESULTS: The major constituents of REO were 1,8-cineole (51.52%), camphor (10.52%), and α-pinene (8.41%). The results revealed a superior hepatoprotective activity of the combination when compared to each natural product alone, as demonstrated by the lowered liver enzymes, lipid peroxidation, mitigated inflammatory and apoptotic biomarkers, and enhanced antioxidant activities. Furthermore, the combination induced the overexpression of MEK and ERK proteins, providing evidence for the involvement of this cascade in the hepatoprotective activity of such natural products. The administration of rosemary essential oil with curcumin enhanced the curcuminoid plasma level. CONCLUSION: The co-administration of both curcumin and rosemary essential oil together enhanced both their hepatoprotective activity and the level of curcumin in plasma, indicating a synergistic activity between both natural products.

The Synthesis, Fungicidal Activity, and in Silico Study of Alkoxy Analogues of Natural Precocenes I, II, and III.

This study aimed to synthesize, characterize, and explore the eco-friendly and antifungal potential of precocenes and their derivatives. The organic synthesis of the mono-O-alkyl-2,2-dimethyl 2H-1-chromene series, including the natural product precocene I, and the di-O-alkyl 2,2-dimethyl-2H-1-chromene series, including the natural 2H-1-chromenes precocenes II and III, was achieved. The synthetic compounds were subjected to spectroscopic analysis, 1HNMR,13CNMR, and mass characterization. The antifungal activity of synthesized precocenes I, II, and III, as well as their synthetic intermediates, was evaluated by the poison food technique. Precocene II (EC50 106.8 µg × mL-1 and 4.94 µg mL-1), and its regioisomers 7a (EC50 97.18 µg × mL-1 and 35.30 µg × mL-1) and 7d (EC50 170.58 × µg mL-1), exhibited strong fungitoxic activity against Aspergillus niger and Rhizoctonia solani. Some of the novel chromenes, 11a and 11b, which had never been evaluated before, yielded stronger fungitoxic effects. Finally, docking simulations for compounds with promising fungitoxic activity were subjected to structure-activity relationship analyses against the polygalactouronases and voltage-dependent anion channels. Conclusively, precocenes and their regioisomers demonstrated promising fungitoxic activity; such compounds can be subjected to minor structural modifications to yield promising and novel fungicides.

Bioprospecting Plant Growth Promoting Rhizobacteria for Enhancing the Biological Properties and Phytochemical Composition of Medicinally Important Crops.

Traditionally, medicinal plants have long been used as a natural therapy. Plant-derived extracts or phytochemicals have been exploited as food additives and for curing many health-related ailments. The secondary metabolites produced by many plants have become an integral part of human health and have strengthened the value of plant extracts as herbal medicines. To fulfil the demand of health care systems, food and pharmaceutical industries, interest in the cultivation of precious medicinal plants to harvest bio-active compounds has increased considerably worldwide. To achieve maximum biomass and yield, growers generally apply chemical fertilizers which have detrimental impacts on the growth, development and phytoconstituents of such therapeutically important plants. Application of beneficial rhizosphere microbiota is an alternative strategy to enhance the production of valuable medicinal plants under both conventional and stressed conditions due to its low cost, environmentally friendly behaviour and non-destructive impact on fertility of soil, plants and human health. The microbiological approach improves plant growth by various direct and indirect mechanisms involving the abatement of various abiotic stresses. Given the negative impacts of fertilizers and multiple benefits of microbiological resources, the role of plant growth promoting rhizobacteria (PGPR) in the production of biomass and their impact on the quality of bio-active compounds (phytochemicals) and mitigation of abiotic stress to herbal plants have been described in this review. The PGPR based enhancement in the herbal products has potential for use as a low cost phytomedicine which can be used to improve health care systems.

Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals.

In recent years, the harmful effects of drought stress have been be mitigated by using bioactive compounds such as antioxidants and osmolytes. In this research, pot experiments were carried out to investigate the effects of ascorbic acid, glutathione and proline on alleviating the harmful effect of drought stress in chickpea plants during season 2017. Chickpea plant seeds were soaked in ascorbic acid (0.75 mM), glutathione (0.75 mM), proline (0.75 mM) singly and/or in sequence combinations for 4 h and then planted in pots. The pots were irrigated with water after seven days (to serve as control), after 14 days (moderate drought stress) and after 28 days (severe drought stress). The sequence combination of antioxidants and proline under drought stress has not been studied yet. The results showed significantly decreased in plant growth, yielding characteristics, photosynthetic pigments and soluble protein content in response to moderate and severe drought stress. Moreover, treatment with antioxidants caused increment the antioxidant enzyme activity, non-enzymatic antioxidant (ascorbic acid and glutathione) contents and endogenous proline in stressed and unstressed plants. In conclusion, The sequence combination of antioxidants and proline caused improvement in plant growth under drought stress by up-regulating the antioxidant defense system and osmolyte synthesis.

Genetic variability and diversity analysis in Oryza sativa L. genotypes using quantitative traits and SSR markers.

The present study was aimed at evaluating the genetic variation and population structure in a collection of 22 rice genotypes. Twenty-two rice genotypes were assessed using quantitative traits and SSR molecular markers for genetic variability and genetic diversity. As for genetic diversity, the genotypes were clarified based on twelve quantitative traits. Clustering produced two large groups: the IR70423-169-2-2 variety was in a branch alone due to its long duration, while, the second group included all rest of genotypes and was split up into two sub-groups. The first sub-group included IR67418-131-2-3-3-3, IR67420-206-3-1-3-3, Giza181, Giza182, Sakha104, and P1044-86-5-3-3-2M. However, pedigree played in divided clustering with Giza181 and Giza182, which were belonging to the Indica type and produced from the same parents. SSR markers produced 87 alleles, with a mean of 4.3 alleles per locus, which were detected in 22 rice genotypes. A higher number of alleles were found with primers RM262, RM244, RM3843, RM212, and RM3330. With an overall mean of 0.837, the polymorphic information content values were high for all SSR markers, ranging from a low of 0.397 for M254 to a high of 0.837 for RM244. The dendogram was divided into six groups according to the types of genotypes, with the pedigree playing a major role for the genetic distance. In order to help breeders choose parents and create suitable hybrids to achieve genetic improvement in crops, particularly rice, SSR is a useful technique for analysing genotype diversity and aiding in the genetic fingerprinting of each variety.

Green synthesis, characterization, and hepatoprotective effect of zinc oxide nanoparticles from Moringa oleifera leaves in CCl4-treated albino rats.

Hepatotoxin carbon tetrachloride (CCl4) causes liver injury. This research aims to create ZnO-NPs using green synthesis from Moringa oleifera (MO) leaves aqueous extract, and chemically prepared and confirming the synthesis by specialized equipment analysis. The sizes formed of ZnO-NPs were 80 and 55 nm for chemical and green methods, respectively. In addition, to study their ability to protect Wistar Albino male rats against oxidative stress exposed to carbon tetrachloride. MO leaf aqueous extract, green synthesized ZnO-NPs, and ZnO-NPs prepared chemically at 100 and 200 mg/kg BW per day were investigated for their hepatoprotective effects on liver enzyme biomarkers, renal biomarkers, antioxidant enzymes, lipid peroxidation, hematological parameters, and histopathological changes. Compared to the control group, all liver and kidney indicators were considerably elevated after the CCl4 injection. However, the activity of antioxidant enzymes in the liver was significantly reduced after the CCl4 injection. These outcomes indicate that MO leaf aqueous extract, greenly synthesized ZnO-NPs, and ZnO-NPs chemically prepared can restore normal liver and kidney function and activity, as well as hematological and antioxidant enzymes. The highest impact on enhancing the hepatoprotective effect was recorded for rats that received green synthesized ZnO-NPs. The increased drug delivery mechanism of green synthesized ZnO-NPs resulted in a higher protective effect than that of MO leaf aqueous extract.

Biosynthesis of zinc oxide nanoparticles via neem extract and their anticancer and antibacterial activities.

In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized using neem leaf aqueous extracts and characterized using transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-Vis), and dynamic light scattering (DLS). Then compare its efficacy as anticancer and antibacterial agents with chemically synthesized ZnO-NPs and the neem leaf extract used for the green synthesis of ZnO-NPs. The TEM, UV-vis, and particle size confirmed that the developed ZnO-NPs are nanoscale. The chemically and greenly synthesized ZnO-NPs showed their optical absorbance at 328 nm and 380 nm, respectively, and were observed as spherical particles with a size of about 85 nm and 62.5 nm, respectively. HPLC and GC-MS were utilized to identify the bioactive components in the neem leaf aqueous extract employed for the eco-friendly production of ZnO-NPs. The HPLC analysis revealed that the aqueous extract of neem leaf contains 19 phenolic component fractions. The GC-MS analysis revealed the existence of 21 bioactive compounds. The antiproliferative effect of green ZnO-NPs was observed at different concentrations (31.25 µg/mL-1000 µg/mL) on Hct 116 and A 549 cancer cells, with an IC50 value of 111 µg/mL for A 549 and 118 µg/mL for Hct 116. On the other hand, the antibacterial activity against gram-positive and gram-negative bacteria was estimated. The antibacterial result showed that the MIC of green synthesized ZnO-NPs against gram-positive and gram-negative bacteria were 5, and 1 µg/mL. Hence, they could be utilized as effective antibacterial and antiproliferative agents.

Algal Extracts for Green Synthesis of Zinc Oxide Nanoparticles: Promising Approach for Algae Bioremediation.

Zinc oxide nanoparticles (ZnO-NPs) possess unique properties, making them a popular material across various industries. However, traditional methods of synthesizing ZnO-NPs are associated with environmental and health risks due to the use of harmful chemicals. As a result, the development of eco-friendly manufacturing practices, such as green-synthesis methodologies, has gained momentum. Green synthesis of ZnO-NPs using biological substrates offers several advantages over conventional approaches, such as cost-effectiveness, simplicity of scaling up, and reduced environmental impact. While both dried dead and living biomasses can be used for synthesis, the extracellular mode is more commonly employed. Although several biological substrates have been successfully utilized for the green production of ZnO-NPs, large-scale production remains challenging due to the complexity of biological extracts. In addition, ZnO-NPs have significant potential for photocatalysis and adsorption in the remediation of industrial effluents. The ease of use, efficacy, quick oxidation, cost-effectiveness, and reduced synthesis of harmful byproducts make them a promising tool in this field. This review aims to describe the different biological substrate sources and technologies used in the green synthesis of ZnO-NPs and their impact on properties. Traditional synthesis methods using harmful chemicals limit their clinical field of use. However, the emergence of algae as a promising substrate for creating safe, biocompatible, non-toxic, economic, and ecological synthesis techniques is gaining momentum. Future research is required to explore the potential of other algae species for biogenic synthesis. Moreover, this review focuses on how green synthesis of ZnO-NPs using biological substrates offers a viable alternative to traditional methods. Moreover, the use of these nanoparticles for industrial-effluent remediation is a promising field for future research.

Physiological response, phytochemicals, antioxidant, and enzymatic activity of date palm (Phoenix dactylifera L.) cultivated under different storage time, harvesting Stages, and temperatures.

The quality of date palm is highly influenced by postharvest techniques, storage, and processing effects. Fruits stored at room temperature result in dehydration, whereas higher temperatures accelerate the enzymatic browning of fruit. This study aimed to enhance postharvest quality of date palms through improved harvesting and storage techniques. The fruits of date palm (Phoenix dactylifera L. cv. Dhakki) were harvested at khalal (mature, firm), rutab (fully ripe), or tamar (dry) stages and stored at different temperatures (12, 18, or 24 °C) for 0, 15, 30, or 45 days. The analysis of the data showed that the studied attributes significantly different at various ripening stages and storage temperatures. The fruits harvested at Khalal stage proved to be the best in retaining moisture content (23.16%), total soluble solids (20.36 oBrix), fruit juice pH (4.97), ascorbic acid (24.65 mg 100 g-1), non-reducing sugars (26.84%), percent acidity (0.39%), antioxidant activity (211.0 mg 100 g-1), total phenolic (40.07 mg100g-1), flavonoids (45.8 mg 100 g-1), tannin (70.7 mg100g-1), catalase (1.82 U g-1), peroxidase (1.4 U g-1), soluble protein (38.2 mg kg-1), brightness (29.9), chroma (16.4), hue angle (34.9), color (16.8), and with minimum weight loss (8.48%) as compared to fruit harvested at Rutab and Tamar stage. Regarding the means for storage temperature, the fruits stored at 12 ± 3 °C retained the highest moisture content (23.2%), total soluble solids (13.5 oBrix), fruit juice pH (5.42), percent acidity (0.29%), ascorbic acid (24.4 mg100g-1), reducing sugars (31.1%), non-reducing sugars (26.5%), antioxidant activity (214.6 mg100g-1), total phenolic (41.6 mg100 g-1), flavonoids (44.7 mg100 g-1), tannin (71.7 mg 100 g-1), catalase (1.56 U g-1), peroxidase (1.21 U g-1), soluble protein (31.8 mg kg-1), brightness (28.8), chroma (15.3), hue angle (29.6), color (16.2),with minimum weight loss (9.91%). It was concluded that for quality fruit production of date palm cv. Dhakki could be harvested at Khalal stage and stored at a temperature of 12 ± 3 °C.

Improvement of postharvest quality, secondary metabolites, antioxidant activity and quality attributes of Prunus persica L. subjected to solar drying and slice thickness.

Peach is a fruit highly appreciated by consumers; however, it is highly perishable, so drying is an alternative to preserve its physical and chemical properties. In this study, the effect of different drying methods (oven, solar, and open sun) and slice thicknesses (0.5, 1.0, and 1.5 cm) on quality, shelf life, color, total phenol, flavonoid, reducing sugar, non reducing sugar, ascorbic acid contents and antioxidant capacity of peach (Prunus persica L. Cv. Indian blood red), were evaluated. The results showed that, 0.5 cm slice thickness recorded the best results with minimum moisture content (%), drying time (7 hrs), titratable acidity (%), reducing sugars (%), non-reducing sugars (%), total soluble solids (°Brix), disease incidence (%), drying rate, TSS-Acid ratio, ascorbic acid, color and flavor, and total phenolic contents. Storage conditions showed significant results on 90 days of storage with maximum total soluble solids (°Brix), TSS-Acid ratio, reducing sugars (%), minimum titratable acidity (%), ascorbic acid, disease incidence (%), and non-reducing sugars (%), while color and flavor also retained. Peach fruits sliced at 0.5 cm thickness and dried in a solar collector may be considered best to retain the bio chemical attributes for 90 days and solar collector considered as environmentally friendly technology.

Application of Potassium Humate and Salicylic Acid to Mitigate Salinity Stress of Common Bean.

In the current study, we investigated the effect of potassium humate (Kh) and salicylic acid (SA) in mitigating the salinity stress of common bean plants. Common bean seedlings were treated with 0.2 g/L SA as a foliar application and 0.3 g/L Kh as a soil application individually or in combination. After 7 days of germination, plants were treated with 50 mM NaCl and normal water as a control. Our results indicate that salt treatment reduced the plant growth (fresh and dry shoots and roots), leaf pigments (total chlorophyll and carotenoids), ascorbic acid (AA), glutathione (GSH), and potassium (K) contents. On the contrary, proline content; sodium (Na); hydrogen peroxide (H2O2); superoxide anion (O2•-); and antioxidant enzymes, including catalase (CAT), peroxidase (POX), and superoxide dismutase (SOD), were increased by saline stress. However, applying either individual Kh and SA or their combination stimulated seedling growth under salinity stress by increasing growth parameters, leaf pigment contents, AA, GSH, proline content, K content, and antioxidant enzymes compared with the control. Additionally, Na content, H2O2, and O2•- were reduced by all applications. The application of the Kh (0.3 g/L) + SA (0.2 g/L) combination was more effective than using the individual compounds. In conclusion, applications of Kh + SA can mitigate salt stress and improve the seedling growth of common bean.

Promotive Role of 5-Aminolevulinic Acid or Salicylic Acid Combined with Citric Acid on Sunflower Growth by Regulating Manganese Absorption.

Manganese (Mn) is an essential nutrient in most organisms. Establishing an effective regulatory system of Mn absorption is important for sustainable crop development. In this study, we selected sunflower as the model plant to explore the effects of 5-aminolevulinic acid (ALA) or salicylic acid (SA) combined with citric acid (CA) on Mn absorption. Six-leaf-old sunflower plants were exposed to 0.8 g kg-1 Mn for one week and then treated with chelating agents, i.e., CA (10 mmol kg-1), and different concentrations of ALA and SA for one week. The results showed that Mn-treated plants had significantly increased H2O2, O2- and MDA contents in leaves compared with the control. Under the Mn + CA treatment, ALA or SA2 significantly activated the antioxidant defense system by increasing SOD, POD and CAT activities in leaves. Moreover, the application of CA significantly increased the Mn uptake in sunflower roots compared with Mn treatment alone; however, did not accelerate the translocation efficiency of Mn from sunflower roots to shoots. Moreover, ultrastructural and RT-qPCR results further demonstrated that ALA/SA could recover the adverse impact of excessive Mn accumulation in sunflowers. Like a pump, ALA/SA regulated the translocation efficiency and promoted the transportation of Mn from roots to shoots. This study provides insights into the promotive role of ALA/SA combined with CA on sunflower growth by regulating Mn absorption, which would be beneficial for regulating Mn absorption in soil with an Mn deficit.

Cognitive- and memory-enhancing effects of Augmentin in Alzheimer's rats through regulation of gene expression and neuronal cell apoptosis.

Introduction: Alzheimer's disease (AD) is the most common type of dementia among older persons. This study looked at how Augmentin affected behavior, gene expression, and apoptosis in rats in which AD had been induced by scopolamine. Methods: The rats were divided into five groups: control, sham, memantine, Augmentin, and pre-Augmentin (the last group received Augmentin before scopolamine administration and was treated with memantine). A Morris water maze was utilized to measure spatial memory in the animals, and real-time quantitative reverse transcription PCR (qRT-PCR) and flow cytometry were employed to analyze gene expression and neuronal cell apoptosis, respectively. Results: Memantine and Augmentin increased spatial memory in healthy rats. The use of scopolamine impaired spatial memory. Both Augmentin and memantine improved spatial memory in AD rats, particularly in the group that received memantine; however, the outcomes were more substantial when Augmentin was administered before scopolamine was given to induce AD. Furthermore, the expression of presenilin-2 (PSEN2) and inositol-trisphosphate 3-kinase B (ITPKB) increased, whereas the expression of DEAD-box helicase 5 (DDX5) fell in the AD-treated groups; however, the results were more substantial after combination therapy. According to flow cytometry studies, Augmentin pre-treatment reduced apoptosis in AD rats. Discussion: The results showed that administering Augmentin to AD rats before memantine improved their spatial memory, reduced neuronal cell death, upregulated protective genes, and suppressed genes involved in AD pathogenesis.

Safety and immunogenicity of the ChAdOx1, MVA-MERS-S, and GLS-5300 DNA MERS-CoV vaccines.

BACKGROUND: The Middle East respiratory syndrome coronavirus (MERS-CoV) is a pathogen associated with an acute respiratory infection that has a high mortality rate in humans. It was first identified in June of 2012 in the Arabian Peninsula. The success of the COVID-19 vaccines has shown that it is possible to take advantage of medical and scientific advances to produce safe and effective vaccines for coronaviruses. This study aimed to examine the safety and immunogenicity of MERS-CoV vaccines. METHODS: The research method Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) was used as the guideline for this study. RevMan 5.4 software was used to perform a meta-analysis of the included studies. The safety was assessed by recording adverse events following vaccination, and the immunogenicity was assessed by using seroconversion. RESULTS: The study included five randomized controlled trials that met the inclusion criteria after screening. The studies had 173 participants and were performed in four countries. The vaccines examined were the ChAdOx1 MERS vaccine, MVA-MERS-S vaccine, and GLS-5300 DNA MERS-CoV vaccine. The meta-analysis showed no significant differences in local adverse effects (all local adverse effects and pain) or systemic adverse effects (all systemic adverse effects, fatigue, and headache) among participants in groups receiving a high-dose vaccine or a low-dose vaccine. There were, however, higher levels of seroconversion in high-dose groups than in low-dose groups (OR 0.16 [CI 0.06, 0.42, p = 0.0002]). CONCLUSION: The findings showed that high doses of current MERS-CoV vaccine candidates conferred better immunogenicity than low doses and that there were no differences in the safety of the vaccines.

Efficacy of the modified vaccinia Ankara virus vaccine and the replication-competent vaccine ACAM2000 in monkeypox prevention.

BACKGROUND: Recently, there has been an uptick in reported cases of monkeypox (Mpox) in Africa and across the globe. This prompted us to investigate the efficacy of the two vaccines that can prevent Mpox, the modified vaccinia Ankara virus (MVA) vaccine and ACAM2000 vaccine. We analyzed them to determine their rates of humoral cell responses, adverse events, and rash reactions and used these factors as the primary indicators. METHODS: This study adapted primary data obtained from the Medline, Google Scholar, and Cochrane Library databases. We included a total of eight studies, three of which explored the ACAM2000 vaccine and five of which explored the JYNNEOS MVA vaccine. RESULTS: There were significant differences in the rates of humoral responses after inoculation by the two vaccines. JYNNEOS MVA vaccine immunization resulted in a statistically significant increased humoral immune response with an effect size of 81.00 (42.80, 119.21) at a 95% CI and a rash reaction with an effect size of 96.50 (42.09, 235.09.21) at a 95% CI. ACAM2000 resulted in a lesser increase in neutralizing antibodies than JYNNEOS MVA vaccine. Similar findings were identified for the rates of adverse reactions, but the difference was not statistically significant. The differences in rash reaction rates in the two vaccination groups were also not statistically significant. CONCLUSION: ACAM2000 and JYNNEOS vaccines have proven to be efficient in preventing Mpox even though variations exist in their modes of action and associated significant effects. The nonreplicating nature of JYNNEOS prevents the occurrence of the adverse effects seen with other vaccines.