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In this study, we propose a single camera-based dual-channel near-infrared (NIR) fluorescence imaging system that produces color and dual-channel NIR fluorescence images in real time. To simultaneously acquire color and dual-channel NIR fluorescence images of two fluorescent agents, three cameras and additional optical parts are generally used. As a result, the volume of the image acquisition unit increases, interfering with movements during surgical procedures and increasing production costs. In the system herein proposed, instead of using three cameras, we set a single camera equipped with two image sensors that can simultaneously acquire color and single-channel NIR fluorescence images, thus reducing the volume of the image acquisition unit. The single-channel NIR fluorescence images were time-divided into two channels by synchronizing the camera and two excitation lasers, and the noise caused by the crosstalk effect between the two fluorescent agents was removed through image processing. To evaluate the performance of the system, experiments were conducted for the two fluorescent agents to measure the sensitivity, crosstalk effect, and signal-to-background ratio. The compactness of the resulting image acquisition unit alleviates the inconvenient movement obstruction of previous devices during clinical and animal surgery and reduces the complexity and costs of the manufacturing process, which may facilitate the dissemination of this type of system.
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Colorantes Fluorescentes , Verde de Indocianina , Animales , Imagen Óptica/métodos , Procesamiento de Imagen Asistido por Computador , FluorescenciaRESUMEN
We developed a single-camera-based near-infrared (NIR) fluorescence imaging device using indocyanine green (ICG) NIR fluorescence contrast agents for image-induced surgery. In general, a fluorescent imaging system that simultaneously provides color and NIR images uses two cameras, which is disadvantageous because it increases the imaging head of the system. Recently, a single-camera-based NIR optical imaging device with quantum efficiency partially extended to the NIR region was developed to overcome this drawback. The system used RGB_NIR filters for camera sensors to provide color and NIR images simultaneously; however, the sensitivity and resolution of the infrared images are reduced by 1/4, and the exposure time and gain cannot be set individually when acquiring color and NIR images. Thus, to overcome these shortcomings, this study developed a compact fluorescent imaging system that uses a single camera with two complementary metal-oxide semiconductor (CMOS) image sensors. Sensitivity and signal-to-background ratio were measured according to the concentrations of ICG solution, exposure time, and camera gain to evaluate the performance of the imaging system. Consequently, the clinical applicability of the system was confirmed through the toxicity analysis of the light source and in vivo testing.
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Verde de Indocianina , Imagen Óptica , Fluorescencia , Colorantes Fluorescentes , Imagen Óptica/métodos , Óxidos , SemiconductoresRESUMEN
Whole slide imaging (WSI) refers to the process of creating a high-resolution digital image of a whole slide. Since digital images are typically produced by stitching image sequences acquired from different fields of view, the visual quality of the images can be degraded owing to shading distortion, which produces black plaid patterns on the images. A shading correction method for brightfield WSI is presented, which is simple but robust not only against typical image artifacts caused by specks of dust and bubbles, but also against fixed-pattern noise, or spatial variations in pixel values under uniform illumination. The proposed method comprises primarily of two steps. The first step constructs candidates of a shading distortion model from a stack of input image sequences. The second step selects the optimal model from the candidates. The proposed method was compared experimentally with two previous state-of-the-art methods, regularized energy minimization (CIDRE) and background and shading correction (BaSiC) and showed better correction scores, as smooth operations and constraints were not imposed when estimating the shading distortion. The correction scores, averaged over 40 image collections, were as follows: proposed method, 0.39 ± 0.099; CIDRE method, 0.67 ± 0.047; BaSiC method, 0.55 ± 0.038. Based on the quantitative evaluations, we can confirm that the proposed method can correct not only shading distortion, but also fixed-pattern noise, compared with the two previous state-of-the-art methods.
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Algoritmos , Procesamiento de Imagen Asistido por Computador , Iluminación , Microscopía/métodos , ColorRESUMEN
We propose an all-fiber-based dual-modal imaging system that combines noncontact photoacoustic tomography (PAT) and optical coherence tomography (OCT). The PAT remotely measures photoacoustic (PA) signals with a 1550-nm laser on the surface of a sample by utilizing a fiber interferometer as an ultrasound detector. The fiber-based OCT, employing a swept-source laser centered at 1310 nm, shares the sample arm of the PAT system. The fiber-optic probe for the combined system was homemade with a lensed single-mode fiber (SMF) and a large-core multimode fiber (MMF). The compact and robust common probe is capable of obtaining both the PA and the OCT signals at the same position without any physical contact. Additionally, the MMF of the probe delivers the short pulses of a Nd:YAG laser to efficiently excite the PA signals. We experimentally demonstrate the feasibility of the proposed dual-modal system with a phantom made of a fishing line and a black polyethylene terephthalate fiber in a tissue mimicking solution. The all-fiber-optic system, capable of providing complementary information about absorption and scattering, has a promising potential in minimally invasive and endoscopic imaging.
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We report on a noncontact photoacoustic imaging system utilizing an all-fiber-optic heterodyne interferometer as an acoustic wave detector. The acoustic wave generated by a short laser pulse via the photoacoustic effect and arriving at the sample surface could be detected with the fiber-optic heterodyne interferometer without physical contact or using an impedance matching medium. A phantom experiment was conducted to evaluate the proposed system, and the initial acoustic pressure distribution was calculated using a Fourier-based reconstruction algorithm. It is expected that the all-fiber-optic configuration of the proposed system can be applied as a minimally invasive diagnostic tool.
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Diagnóstico por Imagen de Elasticidad/instrumentación , Tecnología de Fibra Óptica/instrumentación , Aumento de la Imagen/instrumentación , Interferometría/instrumentación , Técnicas Fotoacústicas/instrumentación , Diseño de Equipo , Análisis de Falla de Equipo , Fantasmas de ImagenRESUMEN
We report herein the fabrication and performance response of a three-dimensional (3-D) intraoral scan probe based on optical coherence tomography (OCT) that enables 3-D structural and functional diagnoses of the human teeth. The OCT system was configured using a swept-source OCT (SS-OCT) with a center wavelength of 1310 nm. The scan probe was built using an MEMS mirror and an optical collimator. The implemented SS-OCT equipped with the MEMS-based scan probe yielded an axial resolution of 10 µm and a scan range of 8 × 8 mm2. Two-dimensional (2-D) cross-sectional images of the teeth were acquired by the scan probe based on the OCT. The 3-D volume image was acquired by combining a series of 2-D images, which includes internal and structural information of the human teeth. To utilize the OCT system as an intraoral scanner, partially overlapped 3-D volume images were sequentially acquired and stitched. The 3-D stitching was implemented based on an iterative closest point algorithm. The feasibility of the intraoral scan probe is demonstrated based on its ability to image and characterize the structure and function of the human teeth.
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Diagnóstico Bucal/métodos , Tomografía de Coherencia Óptica/métodos , Diente/diagnóstico por imagen , Algoritmos , Diseño de Equipo , Humanos , Imagenología Tridimensional/métodos , Tomografía de Coherencia Óptica/instrumentaciónRESUMEN
We present three-dimensional (3-D) in vivo photoacoustic (PA) images of the blood vasculature of a chicken chorioallantoic membrane (CAM) obtained by using a fiber-based noncontact PA tomography system. With a fiber-optic heterodyne interferometer, the system measures the surface displacement of a sample, induced by the PA wave, which overcomes the disadvantage of physical-contact of ultrasonic transducer in a conventional system. The performance of an implemented system is analyzed and its capability of in vivo 3-D bioimaging is presented. At a depth of 2.5 mm in a phantom experiment, the lateral and axial resolutions were measured as 100 and 30 µm, respectively. The lateral resolution became doubled at a depth of 7.0 mm; however, interestingly, the axial resolution was not noticeably deteriorated with the depth. With the CAM experiment, performed under the American National Standards Institute laser safety standard condition, blood vessel structures placed as deep as 3.5 mm were clearly recognized.