Holographic laser Doppler imaging of microvascular blood flow.

We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time-averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency-shifted reference beam, permits frequency-selective imaging in the radio frequency range. These Doppler images are acquired with an off-axis Mach-Zehnder interferometer. Microvascular hemodynamic components mapping is performed in the cerebral cortex of the mouse and the eye fundus of the rat with near-infrared laser light without any exogenous marker. These measures are made from a basic inverse-method analysis of local first-order optical fluctuation spectra at low radio frequencies, from 0 Hz to 100 kHz. Local quadratic velocity is derived from Doppler broadenings induced by fluid flows, with elementary diffusing wave spectroscopy formalism in backscattering configuration. We demonstrate quadratic mean velocity assessment in the 0.1-10 mm/s range in vitro and imaging of superficial blood perfusion with a spatial resolution of about 10 micrometers in rodent models of cortical and retinal blood flow.

Nonlinear collision between propagating waves in mouse somatosensory cortex.

How does cellular organization shape the spatio-temporal patterns of activity in the cortex while processing sensory information? After measuring the propagation of activity in the mouse primary somatosensory cortex (S1) in response to single whisker deflections with Voltage Sensitive Dye (VSD) imaging, we developed a two dimensional model of S1. We designed an inference method to reconstruct model parameters from VSD data, revealing that a spatially heterogeneous organization of synaptic strengths between pyramidal neurons in S1 is likely to be responsible for the heterogeneous spatio-temporal patterns of activity measured experimentally. The model shows that, for strong enough excitatory cortical interactions, whisker deflections generate a propagating wave in S1. Finally, we report that two consecutive stimuli activating different spatial locations in S1 generate two waves which collide sub-linearly, giving rise to a suppressive wave. In the inferred model, the suppressive wave is explained by a lower sensitivity to external perturbations of neural networks during activated states.