A Method for Controlled Odor Delivery in Olfactory Field-Testing.

A widely recognized limitation in mammalian olfactory research is the lack of current methods for measuring odor availability (i.e., the quantifiable amount of odor presented and thus available for olfaction) of training or testing materials during behavioral or operational testing. This research utilized an existing technology known as Controlled Odor Mimic Permeation Systems (COMPS) to produce a reproducible, field-appropriate odor delivery method that can be analytically validated and quantified, akin to laboratory-based research methods, such as permeation devices that deliver a stable concentration of a specific chemical vapor for instrumental testing purposes. COMPS were created for 12 compounds across a range of carbon chain lengths and functional groups in such a way to produce similar permeation rates for all compounds. Using detection canines as a model, field-testing was performed to assess the efficacy of the method. Additionally headspace concentrations over time were measured as confirmation of odor availability using either externally sampled internal standard-solid phase microextraction-gas chromatography-mass spectrometry (ESIS-SPME-GC-MS) or collection onto a programmable temperature vaporizing (PTV) GC inlet with MS detection. Finally, lifetime usage was considered. An efficient method for producing and measuring reliable odor availabilities across various chemical functional groups was developed, addressing a noted gap in existing literature that will advance canine and other nonhuman mammal research testing.

Preliminary accuracy of COVID-19 odor detection by canines and HS-SPME-GC-MS using exhaled breath samples.

The novel coronavirus SARS-CoV-2, since its initial outbreak in Wuhan, China has led to a worldwide pandemic and has shut down nations. As with any outbreak, there is a general strategy of detection, containment, treatment and/or cure. The authors would argue that rapid and efficient detection is critical and required to successful management of a disease. The current study explores and successfully demonstrates the use of canines to detect COVID-19 disease in exhaled breath. The intended use was to detect the odor of COVID-19 on contaminated surfaces inferring recent deposition of infectious material from a COVID-19 positive individual. Using masks obtained from hospitalized patients that tested positive for COVID-19 disease, four canines were trained and evaluated for their ability to detect the disease. All four canines obtained an accuracy >90% and positive predictive values ranging from ~73 to 93% after just one month of training.

Generalization and Discrimination of Molecularly Similar Odorants in Detection Canines and the Influence of Training.

Operationally-deployed canine detectors are often trained on one or a limited number of materials representing a single target odor, and training frequently occurs using materials of a high purity grade in controlled scenarios with minimal other background odors. Conversely, in the field, canine detectors are expected to generalize and identify variations of the target odor, while discriminating from similar extraneous or background odors. This exemplifies the balance between generalization and discrimination required for effective canine detectors. This research explored the tendency for detection canines to generalize or discriminate between similar odorants. Two groups of related odorants were used in two separate studies; (1) odorants of similar functional groups with differing carbon chains, and (2) odorants of similar carbon chain length but differing functional groups. Within each odorant set, the effect of training was addressed by incrementally increasing the number of odorants each canine was trained to detect. Initially, discrimination increased with increasing molecular dissimilarity in both odorant groups. After further training on additional related odorants, generalization increased across the set of odorants of the same carbon chain length, but there were no significant changes in either generalization or discrimination across the set of odorants of the same functional group. The results suggest that the canines in this study were more likely to generalize across compounds of the same chain length with differing functional group than across compounds of the same functional group, but differing chain lengths. Furthermore, some variation in performance between individual canines indicated that the tendency to generalize differed with experience, breed, and other factors affecting olfaction.

Program management and policy issues in information outreach: lessons from Tribal Connections.

With the advent of the Internet, American Indian/Alaska Native (AI/AN) communities in the Pacific Northwest have new opportunities to access high quality and relevant health information. The Pacific Northwest Regional Medical Library (PNRML), regional headquarters of the National Network of Libraries of Medicine, a program sponsored by the National Library of Medicine, sought to facilitate that access and worked with a selected group of sixteen tribes and native village consortia. The steps were: (1) work with AI/AN communities to arrive at mutually-agreeable health information connectivity objectives and long-term solutions, (2) provide funding to AI/AN communities to ensure Internet connectivity and the presence of Internet workstations for health workers and for the public, and (3) train in effective health information seeking. Community-based approaches helped the PNRML adjust policies and practice for improved information outreach to AI/AN communities in the region. The project participants, collaborating with our staff, successfully carried out many of the community goals and, at the same time, we gained insight about the variables that were barriers or facilitators of success. While we are coming at outreach from a library perspective, the policy and method lessons we learned could apply to a broad variety of outreach endeavors.