Neuromuscular Adaptations in Endurance-Trained Male Adolescents Versus Untrained Peers: A 9-Month Longitudinal Study.

BACKGROUND: Neuromuscular function is considered as a determinant factor of endurance performance during adulthood. However, whether endurance training triggers further neuromuscular adaptations exceeding those of growth and maturation alone over the rapid adolescent growth period is yet to be determined. OBJECTIVE: The present study investigated the concurrent role of growth, maturation, and endurance training on neuromuscular function through a 9-month training period in adolescent triathletes. METHODS: Thirty-eight 13- to 15-year-old males (23 triathletes [~6 h/week endurance training] and 15 untrained [<2 h/week endurance activity]) were evaluated before and after a 9-month triathlon training season. Maximal oxygen uptake (V̇O2max) and power at V̇O2max were assessed during incremental cycling. Knee extensor maximal voluntary isometric contraction torque (MVCISO) was measured and the voluntary activation level (VAL) was determined using the twitch interpolation technique. Knee extensor doublet peak torque (T100Hz) and normalized vastus lateralis (VL) electromyographic activity (EMG/M-wave) were also determined. VL and rectus femoris (RF) muscle architecture was assessed using ultrasonography. RESULTS: Absolute V̇O2max increased similarly in both groups but power at V̇O2max only significantly increased in triathletes (+13.8%). MVCISO (+14.4%), VL (+4.4%), and RF (+15.8%) muscle thicknesses and RF pennation angle (+22.1%) increased over the 9-month period in both groups similarly (p < 0.01), although no changes were observed in T100Hz, VAL, or VL EMG/M-wave. No changes were detected in any neuromuscular variables, except for coactivation. CONCLUSION: Endurance training did not induce detectible, additional neuromuscular adaptations. However, the training-specific cycling power improvement in triathletes may reflect continued skill enhancement over the training period.

Constitutional thinness might be characterized by physiologically adapted and not impaired muscle function and architecture: new results from the NUTRILEAN study.

PURPOSE: While muscle mass and skeletal muscle fibers phenotype have been shown atypical in constitutional thinness (CT), force production capacities and its architectural determinants have never been explored. The present study compared muscle functionality and architecture between participants with CT and their normal-weight (NW) counterparts. METHODS: Anthropometry, body composition (Dual-X-ray Absorptiometry), physical activity/sedentary behavior (ActiGraph wGT3X-BT), ultrasound recording of the Vastus Lateralis (2D-ultrasound system), and functional capacities at maximal isometric and isokinetic voluntary contractions (MVCISO and MVCCON) during knee extension (isokinetic dynamometer chair Biodex) have been measured in 18 women with CT (body mass index < 17.5 kg/m2) and 17 NW women. RESULTS: A lower fat-free mass (ES: -1.94, 95%CI: -2.76 to -1.11, p < 0.001), a higher sedentary time, and a trend for a lower time spent at low-intensity physical activity, were observed in CT vs NW participants. While absolute MVCISO, MVCCON, rate of torque development (RTD), and torque work were all markedly lower in CT, these differences disappeared when normalized to body or muscle mass. Muscle thickness and fascicle length were found lower in CT (ES: -1.29, 95%CI: -2.03 to -0.52, p < 0.001; and ES: -0.87, 95%CI: -1.58 to -0.15, p = 0.02, respectively), while pennation angle was found similar. CONCLUSION: Despite lower absolute strength capacities observed in CT, present findings support the hypothesis of physiological adaptations to the low body and muscle mass than to some intrinsic contractile impairments. These results call for further studies exploring hypertrophy-targeted strategies in the management of CT.

Can neuromuscular differences manifest by early adolescence in males between predominantly endurance and strength sports?

INTRODUCTION: Although neuromuscular function varies significantly between strength and endurance-trained adult athletes, it has yet to be ascertained whether such differences manifest by early adolescence. The aim of the present study was to compare knee extensor neuromuscular characteristics between adolescent athletes who are representative of strength (wrestling) or endurance (triathlon) sports. METHODS: Twenty-three triathletes (TRI), 12 wrestlers (WRE) and 12 untrained (CON) male adolescents aged 13 to 15 years participated in the present study. Maximal voluntary isometric contraction (MVIC) knee extensor (KE) torque was measured, and 100-Hz magnetic doublets were delivered to the femoral nerve during and after KE MVIC to quantify the voluntary activation level (%VA). The doublet peak torque (T100Hz) and normalized vastus lateralis (VL) and rectus femoris (RF) EMG (EMG/M-wave) activities were quantified. VL and RF muscle architecture was also assessed at rest using ultrasound. RESULTS: Absolute and relative (to body mass) KE MVIC torques were significantly higher in WRE than TRI and CON (p < 0.05), but comparable between TRI and CON. No significant differences were observed between groups for %VA, T100Hz or either VL or RF muscle thickness. However, VL EMG/M-wave was higher, RF fascicle length longer, and pennation angle smaller in WRE than TRI and CON (all p < 0.05). CONCLUSION: The wrestlers were stronger than triathletes and controls, potentially as a result of muscle architectural differences and a greater neural activation. Neuromuscular differences can already be detected by early adolescence in males between predominantly endurance and strength sports, which may result from selection bias and/or physical training.

Post-exercise heart rate recovery and parasympathetic reactivation are comparable between prepubertal boys and well-trained adult male endurance athletes.

PURPOSE: This study tested the hypothesis that prepubertal boys, but not untrained men, would exhibit a similar post-exercise parasympathetic reactivation as well-trained adult male endurance athletes. METHODS: Twelve prepubertal boys (12.3 ± 1.6 years), 14 untrained men (21.8 ± 2.2 years) and 16 well-trained adult male endurance athletes (24.5 ± 4.8 years) completed an incremental maximal run field test on a track. Immediately after exercise completion, heart rate recovery (HRR) was assessed in the supine position for 5 min. Heart rate variability was analyzed in the time domain, and log-transformed values of the root mean square of successive differences in heart beats (Ln RMSSD30) were calculated over consecutive 30 s windows. RESULTS: Prepubertal children and well-trained adult endurance athletes showed significantly faster HRR than untrained adults from 30 s post-exercise until the end of recovery (p < 0.05). Ln RMSSD30 was significantly higher in prepubertal children and athletes than untrained adults over the post-exercise time interval 60-150 s (p < 0.05). No significant differences were observed for HRR and Ln RMSSD30 between prepubertal children and athletes. CONCLUSION: Prepubertal children and well-trained adult endurance athletes exhibited comparable and faster HRR and parasympathetic reactivation than untrained adults following maximal exercise. This indirectly suggests that oxidative profile may be preserved by exercise training during growth and maturation to offset the decline in post-exercise HRR, parasympathetic reactivation and aspects of metabolic health.

Locomotor activities as a way of inducing neuroplasticity: insights from conventional approaches and perspectives on eccentric exercises.

Corticospinal excitability, and particularly the balance between cortical inhibitory and excitatory processes (assessed in a muscle using single and paired-pulse transcranial magnetic stimulation), are affected by neurodegenerative pathologies or following a stroke. This review describes how locomotor exercises may counterbalance these neuroplastic alterations, either when performed under its conventional form (e.g., walking or cycling) or when comprising eccentric (i.e., active lengthening) muscle contractions. Non-fatiguing conventional locomotor exercise decreases intracortical inhibition and/or increases intracortical facilitation. These modifications notably seem to be a consequence of neurotrophic factors (e.g., brain-derived neurotrophic factor) resulting from the hemodynamic solicitation. Furthermore, it can be inferred from non-invasive brain and peripheral stimulation studies that repeated activation of neural networks can endogenously shape neuroplasticity. Such mechanisms could also occur following eccentric exercises (lengthening of the muscle), during which motor-related cortical potential (electroencephalography) is of greater magnitude and lasts longer than during concentric exercises (i.e., muscle shortening). As single-joint eccentric exercise decreased short- and long-interval intracortical inhibition and increased intracortical facilitation, locomotor eccentric exercise (e.g., downhill walking or eccentric cycling) may be even more potent by adding hemodynamic-related neuroplastic processes to endogenous processes. Besides, eccentric exercise is especially useful to develop relatively high force levels at low cardiorespiratory and perceived intensities, which can be a training goal alongside the induction of neuroplastic changes. Even though indirect evidence let us think that locomotor eccentric exercise could shape neuroplasticity in ways relevant to neurorehabilitation, its efficacy remains speculative. We provide future research directions on the neuroplastic effects and underlying mechanisms of locomotor exercise.

Neuromuscular Fatigue After Long-Duration Adventure Racing in Adolescent Athletes.

PURPOSE: To characterize the acute effects of a long-duration adventure race on knee extensor (KE) fatigue and the knee functional ratio in adolescent athletes. METHODS: Twenty trained male adolescents (aged 14-17 y) performed an adventure race of 68.5 km. Maximal voluntary isometric contraction (MVIC) KE and knee flexor torques were measured before and immediately after the race. Central and peripheral components of neuromuscular fatigue were quantified from the maximal voluntary activation level and the doublet peak torque (Tw100), respectively. The peak eccentric knee flexor torque to concentric KE torque ratio was also measured to determine functional ratio. RESULTS: The race completion time was 05:38 (00:20) hours. Significant reductions in MVICKE (-14.7%, P < .001) and MVICKF (-17.0%, P < .01) were observed after the race. Voluntary activation level decreased by 8.3% (P < .001) while Tw100 remained unchanged. Peak eccentric knee flexor torque decreased 16.0% (P < .001) while peak concentric KE torque did not change. This resulted in a significant reduction in functional ratio (-12.0%, P < .01). CONCLUSION: The adventure race induced a moderate fatigue, which was mainly explained by central factors without significant peripheral fatigue. However, particular attention should be paid to the knee muscular imbalance incurred by the race, which could increase the risk of ligament injury in adolescent athletes.

Corticospinal excitability changes following downhill and uphill walking.

Locomotor exercise may induce corticospinal excitability and/or cortical inhibition change in the knee extensors. This study investigated whether the mode of muscle contraction involved during a locomotor exercise modulates corticospinal and intracortical responsiveness. Eleven subjects performed two 45-min treadmill walking exercises in an uphill (+ 15%) or a downhill (- 15%) condition matched for speed. Maximal voluntary isometric torque (MVIC), voluntary activation level (VAL), doublet (Dt) twitch torque, and M-wave area of the knee extensors were assessed before and after exercise. At the same time-points, motor-evoked potential (MEP), cortical silent period (CSP), and short-interval cortical inhibition (SICI) were recorded in the vastus lateralis (VL) and rectus femoris (RF) muscles. After exercise, uphill and downhill conditions induced a similar loss in MVIC torque (- 9%; p < 0.001), reduction in VAL (- 7%; p < 0.001), and in M-wave area in the VL muscle (- 8%; p < 0.001). Dt twitch torque decreased only after the downhill exercise (- 11%; p < 0.001). MEP area of the VL muscle increased after the downhill condition (p = 0.007), with no change after the uphill condition. MEP area of the RF muscle remained stable after exercises. CSP and SICI did not change in the two conditions for both muscles. Downhill walking induces an increase in MEP area of the VL muscle, with no change of the CSP duration or SICI ratio. The eccentric mode of muscle contraction during a locomotor exercise can modulate specifically corticospinal excitability in the knee extensors.

Neuromuscular and perceptual responses to moderate-intensity incline, level and decline treadmill exercise.

PURPOSE: To describe the neuromuscular and perceptual responses to incline, decline or level treadmill exercise. METHODS: Fifteen healthy subjects performed on separate days 45 min treadmill exercise at 75% heart rate reserve in a level (+ 1% slope), incline (+ 15%) or decline condition (- 15%). Neuromuscular function of the knee extensors (KE) was assessed before and after exercise. Perception of effort, muscle pain and pleasure were measured during the exercise. Muscle pain was also reported up to 96 h after exercise. RESULTS: At the same heart rate, the decline exercise was performed at a higher velocity. This higher velocity was associated with a higher perceived effort and muscle pain, as well as lower pleasure. Maximal isometric KE peak torque and maximal voluntary activation similarly decreased in the three conditions (~ 15 ± 12 and ~ 4 ± 4%). M-wave amplitude of the vastus medialis muscle decreased in the three conditions (~ - 12 ± 13%). M-wave amplitude of the rectus femoris muscle decreased only after the decline exercise (- 12 ± 16%). Peak twitch torque of the electrically evoked contractions was reduced after incline and decline exercises for both 10 and 100 Hz doublets (- 8 ± 9 and - 17 ± 18%). The Dt10/Dt100 ratio was reduced only after decline exercise (- 24 ± 19%). CONCLUSION: At the same moderate intensity, decline exercise induced a greater level of muscle fatigue associated with a higher perceived effort and muscle pain than incline and level exercise. Exercise intensity should be carefully monitored during decline locomotion for training or rehabilitation purposes.

Impact of long-duration adventure racing on hydration status, blood electrolytes and biomarkers of kidney function in trained adolescent athletes.

BACKGROUND: Little is known about the biochemical consequences of endurance activities in adolescents. The present study aimed to examine the impact of a long-duration adventure race (>5 h) on hydration status, blood electrolytes and biomarkers of kidney function in adolescent athletes. METHODS: Twenty male adolescents aged 14 to 17 y (mean±SD; body mass: 59.7±9.1 kg and maximal O2 uptake: 56.2±4.6 mL∙kg-1∙min-1) volunteered to participate in a competitive adventure race of 68.5 km. Volunteers could drink ad libitum and fluid intake was monitored throughout the race. Blood samples were collected before, within 15 minutes after, and 24 hours after the race to monitor blood electrolytes (sodium, potassium, chloride), creatinine and blood urea nitrogen (BUN). Body mass and urine specific gravity (USG) were also measured across the same time points. RESULTS: The race was completed on average in 05:38±00:20 h:min under cold and rainy conditions (10-15 °C and 83-93% of relative humidity). Fluid intake was 1.45±0.66 L and body mass decreased by 1.2% compared to before the race (P<0.001). Blood sodium concentrations remained stable after the race (140.4±2.1 mmol∙L-1) despite an expansion in the plasma volume of 8.9±15.6%. No significant variations in BUN or BUN-to-creatinine ratio occurred. A significant increase in creatinine (+13.5%, P=0.003) was observed immediately after the race but remained within the reference range. CONCLUSIONS: The long-duration race completed under cold and humid conditions seems not to have exposed adolescents to hypohydration, hyponatremia or clinically significant alterations in kidney function.

Effect of the Knee and Hip Angles on Knee Extensor Torque: Neural, Architectural, and Mechanical Considerations.

This study examined the influence of knee extensors' hip and knee angle on force production capacity and their neuromuscular and architectural consequences. Sixteen healthy men performed sub-maximal and maximal voluntary isometric contractions (MVIC) of knee extensors with four different combinations of the knee and hip angles. Muscle architecture, excitation-contraction coupling process, muscular activity, and corticospinal excitability were evaluated on the vastus lateralis (VL) and rectus femoris (RF) muscles. MVIC and evoked peak twitch (Pt) torques of knee extensors increased significantly (p < 0.05) by 42 ± 12% and 47 ± 16% on average, respectively, under knee flexed positions (110° flexion, 0° = full extension) compared to knee extended positions (20° flexion) but were not different between hip positions (i.e., 0° or 60° flexion). Knee flexion also affected VL and RF muscle and fascicle lengths toward greater length than under knee extended position, while pennation angle decreased for both muscles with knee flexion. Pennation angles of the VL muscle were also lower under extended hip positions. Alternatively, no change in maximal muscle activation or corticospinal activity occurred for the VL and RF muscles across the different positions. Altogether these findings evidenced that MVIC torque of knee extensors depended particularly upon peripheral contractile elements, such as VL and RF muscle and fascicle lengths, but was unaffected by central factors (i.e., muscle activation). Furthermore, the hip position can affect the pennation angle of the VL, while VL muscle length can affect the pennation angle of the RF muscle. These elements suggest that the VL and RF muscles exert a mutual influence on their architecture, probably related to the rectus-vastus aponeurosis.

Corticospinal changes induced by fatiguing eccentric versus concentric exercise.

The present study assessed neuromuscular and corticospinal changes during and after a fatiguing submaximal exercise of the knee extensors in different modes of muscle contraction. Twelve subjects performed two knee extensors exercises in a concentric or eccentric mode, at the same torque and with a similar total impulse. Exercises consisted of 10 sets of 10 repetitions at an intensity of 80% of the maximal voluntary isometric contraction torque (MVIC). MVIC, maximal voluntary activation level (VAL) and responses of electrically evoked contractions of the knee extensors were assessed before and after exercise. Motor evoked potential amplitude (MEP) and cortical silent period (CSP) of the vastus medialis (VM) and rectus femoris (RF) muscles were assessed before, during and after exercise. Similar reductions of the MVIC (-13%), VAL (-12%) and a decrease in the peak twitch (-12%) were observed after both exercises. For both VM and RF muscles, MEP amplitude remained unchanged during either concentric or eccentric exercises. No change of the MEP amplitude input-output curves was observed post-exercise. For the RF muscle, CSP increased during the concentric exercise and remained lengthened after this exercise. For the VM muscle, CSP was reduced after the eccentric exercise only. For a similar amount of total impulse, concentric and eccentric knee extensor contractions led to similar exercise-induced neuromuscular response changes. For the two muscles investigated, no modulation of corticospinal excitability was observed during or after either concentric or eccentric exercises. However, intracortical inhibition showed significant modulations during and after exercise.

An approach to a muscle force model with force-pulse amplitude relationship of human quadriceps muscles.

BACKGROUND: Recent advanced applications of the functional electrical stimulation (FES) mostly used closed-loop control strategies based on mathematical models to improve the performance of the FES systems. In most of them, the pulse amplitude was used as an input control. However, in controlling the muscle force, the most popular force model developed by Ding et al. does not take into account the pulse amplitude effect. The purpose of this study was to include the pulse amplitude in the existing Ding et al. model based on the recruitment curve function. METHODS: Quadriceps femoris muscles of eight healthy subjects were tested. Forces responses to stimulation trains with different pulse amplitudes (30-100 mA) and frequencies (20-80 Hz) were recorded and analyzed. Then, specific model parameter values were identified by fitting the measured forces for one train (50 Hz, 100 mA). The obtained model parameters were then used to identify the recruitment curve parameter values by fitting the force responses for different pulse amplitudes at the same frequency train. Finally, the extended model was used to predict force responses for a range of stimulation pulse amplitudes and frequencies. RESULTS: The experimental results indicated that our adapted model accurately predicts the force-pulse amplitude relationship with an excellent agreement between measured and predicted forces (R2=0.998, RMSE = 6.6 N). CONCLUSIONS: This model could be used to predict the pulse amplitude effect and to design control strategies for controlling the muscle force in order to obtain precise movements during FES sessions using intensity modulation.

Changes in cortico-spinal excitability following uphill versus downhill treadmill exercise.

An acute bout of aerobic exercise induces neuroplasticity in the motor cortex. Moreover, paired associative stimulation (PAS) is known to induce neuroplasticity in M1. However, the possible influence of the type of exercise on the neuroplastic changes remains unknown. The present study investigated the effects of two different modes of muscle contraction produced during locomotor exercise on changes in corticospinal (CS) excitability. Subjects performed two 30-min treadmill exercises at an intensity corresponding to 60% of their maximal heart rate with either a +10% (uphill) or -10% (downhill) slope. These exercises were followed or not by paired associative stimulation method (PAS25) which consisted of 200 paired stimuli (0.25Hz, 15min) of median nerve electrical stimulation followed by transcranial magnetic stimulation of the hand M1 area (ISI 25ms). Motor evoked potentials (MEP), assessed through abductor pollicis brevis (APB) activity were obtained before exercise, at 5min, 15min and 30min after exercise. A significant (P<0.05) increase of the MEP amplitude was observed 30min after both exercises but was not different between the two modes of locomotion. On the contrary, MEP amplitude with PAS25 increased only 30min after downhill exercise. We conclude that sub-maximal treadmill exercise increases CS excitability within a period of 30min. However, the predominant mode of muscle contraction during uphill versus downhill locomotion does not influence CS excitability when assessed using a non-exercised muscle. However, results from PAS25 suggest that specific neuroplastic changes occur likely due to homeostatic mechanisms induced by exercise plus a PAS protocol.