Data on the effects of the probiotic "Lacto Sacc".

The effects of the probiotic "Lacto Sacc" on the lactic acid concentration, pH value and microbiological status of the intestinal content and faeces, as well as the ileal digestibility of different nutrients and essential amino acids were studied in growing pigs into which a T-cannula had been inserted surgically at the proximal part of the duodenum and at the distal part of the ileum. No differences were found between the control and the experimental group in the measured parameters of the chyme samples taken from the duodenum. As a result of "Lacto Sacc" feeding, lactic acid concentration of the ileal content increased from 4.77% to 7.22% while its pH value decreased significantly (P < 0.05) from 7.39 to 6.42. The Streptococcus count was also significantly (P < 0.01) elevated. At the same time, the Escherichia coli count decreased significantly (P < 0.001) as a function of the elevated lactic acid concentration. The ileal digestibility of nutrients and essential amino acids improved by 1.3-16.1%. The favourable change observed in the microbiological status is attributed to the higher lactic acid concentration and the resulting lower pH, which adversely affect the survival of E. coli. Further studies are needed to reveal the factors responsible for the improved digestibility of nutrients.

[Fluorescence in situ hybridization in prenatal diagnosis. First experiences]. / A fluoreszcens in situ hibridizáció alkalmazása a praenatalis diagnosztikában. Elsö tapasztalatok.

The authors examined the potential use of non-radioactive in situ hybridization in prenatal screening. Probes for chromosomes 18, 13/21, 21 and X were applied on fourteen samples of peripheral lymphocytes and nine samples of chorionic villi. The aim of the study was to compare the analyzability of the two different DNA probes for chromosome 21 on six samples of chorionic villi. Six of the nine samples of chorionic villi were hybridized with probes specific for chromosome 21 and all six cases were diagnosed properly. However, we need more data to establish a screening protocol for routine prenatal cytogenetics.

[Intrauterine management of Rh-alloimmunization]. / Az Rh-alloimmunizáció intrauterin kezelése.

The authors report the strategy of invasive management of Rh alloimmunisation in pregnancy. From the 34 pregnancies 6 were monitored by amniocenteses, 11 by fetal blood sampling, and 4 with combination of the two above mentioned diagnostic procedures. In 13 cases the fetuses were treated with intrauterine intravascular blood transfusions. All the procedures were ultrasound guided. The fetal blood sampling and the transfusions were carried out by puncturing the umbilical vein or artery. For transfusions, maternal blood was used in case of identical blood type, otherwise adult Rh negative, filtered, washed, irradiated blood was transfused. They report the complications as well, giving the cause of their fetal losses in details. There were no maternal complications observed. Out of the 34 pregnant women 25 had healthy newborns, which number is acceptable in this disease with a very high mortality rate. The authors underline that the technique of fetal blood sampling and intrauterine transfusion if needed is necessary in the management of Rh alloimmunised pregnancies.

AZFc deletion detected in a newborn with prenatally diagnosed Yq deletion.

A case of prenatally diagnosed Yq deletion is described. Fluorescence in situ hybridisation (FISH) was used to identify the abnormal chromosome and to exclude mosaicism. Based on the cytogenetic result and the ultrasound investigation the pregnancy was continued. A newborn with normal male genitalia was delivered. Microdeletion analysis of the Yq showed the absence of the AZFc region. This type of deletion has been described as being associated with azoospermia or oligozoospermia with a progressive decrease of sperm number over time. Long-term andrological follow-up of the newborn will be necessary with eventual cryoconservation of sperm at early adulthood. The present report proposes that AZF analysis combined with FISH has an important role in accurate genetic counselling in sex chromosome anomalies.