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PURPOSE: The purpose of this study was to evaluate the flexural strength (FS), flexural modulus (FM), and fatigue limit (FL) of 3D-printed resin-based polymers and composites and compare them to 3D-printed composites. MATERIALS AND METHODS: A bar-shaped specimen (25 × 2 × 2 mm) was CAD designed according to ISO 4049:2019, and 60 duplicates of the 3D model were nested at a 45-degree angle with the printing platform and 3D-printed with three materials: denture teeth resin (Denture Teeth, Formlabs), temporary crown and bridge resin (Temporary CB, Formlabs), and composite (Flexcera Smile Ultra+, Desktop Health). The 3D model was also imported into a dental CAM software, duplicated 60 times, nested, and milled from a 3D-milled composite puck (Ivotion Denture Teeth, Ivoclar). All specimens were post-processed following the manufacturer's recommendation. The specimens were then subjected to a three-point bending test until failure using a Universal Testing Machine at a crosshead speed of 0.75 mm/min, and FS and FM were calculated. The remaining thirty specimens were tested for Fatigue Limit using the staircase approach starting at 50% FS maximum up to 1.2 M cycles at 10 Hz. The data were analyzed using one-way ANOVA and the Weibull distribution (α = 0.05). RESULTS: The results showed that Ivotion and Flexcera had higher FS (110.3 ± 7.1 MPa and 107.6 ± 6.4 MPa, respectively) and FM (3.3 ± 0.1 GPa and 3.0 ± 0.2 GPa, respectively) compared to the 3D-printed Denture Teeth (FS = 66.4 ± 18.5 MPa and FM = 1.8 ± 0.1 GPa) and Temporary CB (FS = 79.6 ± 12.1 MPa and FM = 2.7 ± 0.4 GPa). Weibull analysis showed that the Ivotion and Flexcera had a more uniform and narrower spatial distribution of defects (m: 27.98 and 29.19) than the printed materials, which had m values of 8.17 and 4.11 for Temporary CB and Denture Teeth, respectively. Although no differences were found in the static properties (FS and FM) between Ivotion and Flexcera, Ivotion presented a higher endurance limit than Flexcera (51.43 vs. 40.95 MPa). The Temporary CB presented 21.08 MPa and Denture Teeth presented 17.80 MPa of endurance limit. CONCLUSIONS: 3D-milled (Ivotion Denture Teeth) and 3D-printed (Flexcera Smile Ultra+) composites outperformed 3D-printed resins (Formlabs Denture Teeth and Temporary Crown & Bridge) in terms of flexural properties and fatigue resistance. 3D-milled (Ivotion) and 3D-printed (Flexcera) composites exhibited similar flexural properties, but 3D-milled composites showed a 25% higher fatigue endurance limit, suggesting improved clinical longevity.
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OBJECTIVES: The aim of this study was to review available evidence for Type 1A (immediate implant placement and immediate loading) of single tooth replacement in the maxillary esthetic zone. MATERIALS AND METHODS: An electronic search was conducted utilizing the databases of MEDLINE, Embase, and Cochrane to identify publications reporting on the outcomes of Type 1A for single tooth replacement in the maxillary esthetic zone. The success and survival rates of the included articles were reported, which were further categorized according to the clinical criteria reported in Type 1A. Mean survival rates were univariately compared between risk groups and additionally between studies published before and since 2012 using bias-corrected and study size-weighed bootstrap tests. A study time-correcting meta-analysis was then performed to obtain an overall effect for the study pool. RESULTS: A total of 3118 publications were identified in the search, with a total of 68 articles included. A mean number of implants per study were 37.2 and mean follow-up was 2.8 years. All the included studies utilizing Type 1A report highly selective inclusion and exclusion criteria. Univariate risk group comparison determined that studies before 2012 report a significantly lower mean survival rate (difference of -1.9 percentage points [PP], 95% CI: [-0.3, -4.0], p = .02), facial gap dimension had an impact on survival rates (+3.1 PP [0.2, 5.3] for width >2 mm, p = .04), as well as presence of endodontic infection (+2.6 PP [0.9, 5.1], p = .004). CONCLUSIONS: Type 1A has a high survival rate in studies reporting strict patient and site selection criteria. Further research is required to assess esthetic and functional success with Type 1A treatments.
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Implantes Dentales , Humanos , Selección de Paciente , Estética Dental , Bases de Datos FactualesRESUMEN
OBJECTIVES: Working Group 5 was convened to discuss and find consensus on the topics of implant placement and loading protocols associated with single missing teeth in the anterior maxilla (aesthetic zone). Consensus statements, clinical recommendations, patient perspectives and future research suggestions were developed and presented to the plenary for discussion and approval. MATERIALS AND METHODS: Two systematic reviews were developed and submitted prior to the conference. The group considered in detail the systematic reviews and developed statements, clinical recommendations, patient perspectives and future research suggestions based on the findings of the reviews and experience of group members. Definitive versions were developed after presentation to and discussion by the plenary. RESULTS: Five consensus statements were developed and approved from each systematic review. Twelve clinical recommendations were developed by the group based on both reviews and experience. Three patient perspectives were developed, and five suggestions made for future research. CONCLUSIONS: Based on the findings of the systematic reviews and experience of group members, the Type 1A protocol (immediate placement and immediate loading), when utilized in the anterior maxilla under favorable conditions, is considered predictable and is associated with high survival rates. The procedure is considered clinically viable and is associated with aesthetic outcomes, although surgical, technical, and biological complications can occur.
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Implantes Dentales , Pérdida de Diente , Humanos , Consenso , Guías de Práctica Clínica como Asunto , Revisiones Sistemáticas como AsuntoRESUMEN
The present clinical report describes the rehabilitation of a patient diagnosed with ectodermal dysplasia performed by an interdisciplinary team in a comprehensive approach aided by digital technology. The complexity of the treatment was related to predictability regarding timing and the type of approach. The patient was referred for treatment because of congenitally missing and abnormally shaped permanent teeth. The need for an interdisciplinary team involving orthodontic, periodontic, and prosthodontic specialists was identified. A virtual treatment plan was developed to guide tooth movement, placement of dental implants, and tooth preparation for indirect restorations. Therefore, each treatment phase could be communicated to the patient and treatment team in a predictable way.
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Anodoncia , Displasia Ectodérmica , Displasia Ectodérmica/complicaciones , Humanos , Planificación de Atención al Paciente , Prostodoncia , Técnicas de Movimiento DentalRESUMEN
Treatment planning for the transition of patients from terminal dentition to full-arch implant rehabilitation poses challenges. Such challenges pertain to achieving the new orientation of the occlusal and esthetic plane as well as the change of vertical dimension of occlusion (VDO), while the fixed provisionalization using a digital workflow still tends to be considered complex and hard to perform. This article illustrates step-by-step the utilization of a digital workflow protocol in the treatment planning for rehabilitation of terminal dentition patients, simplifying the smile design and ensuring that fixed provisionalization serves both functional and esthetic requirements. This protocol includes facially driven, three-dimensional (3D) digital smile design and chairside mock-up restoration workflows that enable prosthetically driven assessment prior to implant treatment planning and 3D printing of surgical templates and prefabricated interim prostheses, which can predictably reduce chairside time and adjustments at the surgical and fixed provisionalization appointment.
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Implantes Dentales , Prótesis Dental de Soporte Implantado , Diseño Asistido por Computadora , Dentición , Estética Dental , Humanos , Flujo de TrabajoRESUMEN
The increased precision and design of surgical templates for static computer-aided implant surgery has led to concerns of excessive heat generation from the friction between the twist drills, metal sleeves in the surgical template, and guide handles during osteotomy preparation. In addition, the presence of a surgical template can prevent proper cooling of the twist drill as it enters the osteotomy site, leading to greater heat generation. This excessive heat may result in bone necrosis and failure of osseointegration to the implant surface. This article describes a straightforward technique of incorporating an existing design feature in many virtual implant planning software programs to add an irrigation channel to the implant surgical template for effective cooling during osteotomy preparation.
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Implantes Dentales , Implantación Dental Endoósea , Calor , Humanos , Osteotomía , Temperatura , Irrigación TerapéuticaRESUMEN
Total Suspended Solids (TSS) and chlorophyll-a concentration are two critical parameters to monitor water quality. Since directly collecting samples for laboratory analysis can be expensive, this paper presents a methodology to estimate this information through remote sensing and Machine Learning (ML) techniques. TSS and chlorophyll-a are optically active components, therefore enabling measurement by remote sensing. Two study cases in distinct water bodies are performed, and those cases use different spatial resolution data from Sentinel-2 spectral images and unmanned aerial vehicles together with laboratory analysis data. In consonance with the methodology, supervised ML algorithms are trained to predict the concentration of TSS and chlorophyll-a. The predictions are evaluated separately in both study areas, where both TSS and chlorophyll-a models achieved R-squared values above 0.8.
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Clorofila A/química , Aprendizaje Automático , Tecnología de Sensores Remotos/métodos , Algoritmos , Monitoreo del Ambiente , Sistemas de Información Geográfica , Procesamiento de Imagen Asistido por Computador , Calidad del AguaRESUMEN
Spectral information provided by multispectral and hyperspectral sensors has a great impact on remote sensing studies, easing the identification of carbonate outcrops that contribute to a better understanding of petroleum reservoirs. Sensors aboard satellites like Landsat series, which have data freely available usually lack the spatial resolution that suborbital sensors have. Many techniques have been developed to improve spatial resolution through data fusion. However, most of them have serious limitations regarding application and scale. Recently Super-Resolution (SR) convolution neural networks have been tested with encouraging results. However, they require large datasets, more time and computational power for training. To overcome these limitations, this work aims to increase the spatial resolution of multispectral bands from the Landsat satellite database using a modified artificial neural network that uses pixel kernels of a single spatial high-resolution RGB image from Google Earth as input. The methodology was validated with a common dataset of indoor images as well as a specific area of Landsat 8. Different downsized scale inputs were used for training where the validation used the ground truth of the original size images, obtaining comparable results to the recent works. With the method validated, we generated high spatial resolution spectral bands based on RGB images from Google Earth on a carbonated outcrop area, which were then properly classified according to the soil spectral responses making use of the advantage of a higher spatial resolution dataset.
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Geodetic networks provide accurate three-dimensional control points for mapping activities, geoinformation, and infrastructure works. Accurate computation and adjustment are necessary, as all data collection is vulnerable to outliers. Applying a Least Squares (LS) process can lead to inaccuracy over many points in such conditions. Robust Estimator (RE) methods are less sensitive to outliers and provide an alternative to conventional LS. To solve the RE functions, we propose a new metaheuristic (MH), based on the Vortex Search (IVS) algorithm, along with a novel search space definition scheme. Numerous scenarios for a Global Navigation Satellite Systems (GNSS)-based network are generated to compare and analyze the behavior of several known REs. A classic iterative RE and an LS process are also tested for comparison. We analyze the median and trim position of several estimators, in order to verify their impact on the estimates. The tests show that IVS performs better than the original algorithm; therefore, we adopted it in all subsequent RE computations. Regarding network adjustments, outcomes in the parameter estimation show that REs achieve better results in large-scale outliers' scenarios. For detection, both LS and REs identify most outliers in schemes with large outliers.
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PURPOSE: To perform a systematic review on studies assessing clinical outcomes in patients rehabilitated with complete-arch fixed implant-supported prostheses according to the time of loading. MATERIALS AND METHODS: Data obtained from patient and clinical outcomes, as implant failure, success rate, survival rate, biological complications, technical complications, mechanical complications, and marginal bone loss, were included on this review. The search was performed on databases PubMed, Scopus, and Cochrane. Cochrane Collaboration tool was used to assess the risk of bias of randomized controlled studies, and an adapted version of Newcastle-Ottawa scale was used for observational studies. All data were tabulated according to the time of loading: (1) immediate restoration/loading, (2) early loading, and (3) conventional loading. RESULTS: From a total of 4027 studies identified through the three databases, six of them were randomized controlled trials, five of them were prospective observational studies, and another five were retrospective observational studies. In total, 5954 implants, 1294 patients and 1305 full-arch fixed implant-supported prostheses were included in this review. There was a wide heterogeneity among clinical studies regarding the study design and treatment procedures. Thus, pooled estimates were not performed in order to avoid potential biases. The methodological assessment by the Modified Newcastle-Ottawa scale showed a moderate quality of observational studies. Regarding the RCTs studies, all of them presented at least one element of bias according to the Cochrane Collaboration tool for assessing risk of bias. CONCLUSION: There is evidence of high survival-success implant rate (95-100%) for either loading protocols (immediate restoration/loading, early loading, and conventional loading). However, careful attention must be taken by clinician when interpreting the results reported in clinical studies. Future studies should be performed using standardized methodology in order to determine the true predictability regarding immediate, early, and conventional loading protocols.
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Implantes Dentales , Prótesis Dental de Soporte Implantado , Carga Inmediata del Implante Dental , Implantación Dental Endoósea , Fracaso de la Restauración Dental , Estudios de Seguimiento , Humanos , Estudios Observacionales como Asunto , Estudios Prospectivos , Ensayos Clínicos Controlados Aleatorios como Asunto , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
The Eurylaimides is one of the few passerine groups with a pantropical distribution. In this study, we generated a multi-calibrated tree with 83% of eurylaimid species diversity based on 30 molecular loci. Particular attention was given to the monotypic Sapayoidae to reconstruct the biogeography of this radiation. We conducted several topological tests including nonoverlapping subsampling of the concatenated alignment and coalescent species tree reconstruction. These tests firmly placed the South American Sapayoidae as the sister group to all other Eurylaimides families (split at â¼28 Ma), with increasing branch support as highly variable sites were removed. This topology is consistent with the breakup of the insular connection between Africa and South America (Atlantogea) that took place between the middle Eocene and the early Oligocene. We recovered Africa as the cradle of the core Eurylaimides, and this result is supported by all African lineages corresponding to the oldest splits within each family in this group. Our timescale suggests that desertification and the uplift of the Tibetan Plateau caused a parallel divergence between African and Asian lineages in all major clades in the core Eurylaimides at 22-9 Ma. We also propose that the ground-foraging behavior in the Pittidae ancestor allowed the pitta lineage to thrive and coexist with the older arboreal lineages of the core Eurylaimides. In contrast, the diversification of pittas in Australia was likely hindered by direct competition with the endemic ground-foraging oscines that had been well established in that continent since the Eocene.
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Passeriformes/genética , África , Animales , Australia , Evolución Biológica , Fósiles , Variación Genética , Filogenia , Análisis de Secuencia de ADN , América del SurRESUMEN
BACKGROUND INFORMATION: The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose structure and composition was further analysed by electron microscopy and mass spectrometry. RESULTS: Electron microscopy of negatively stained samples revealed that the costa, which is a periodic structure with alternating electron-dense and electron-lucent bands, displays three distinct regions, named the head, neck and body. Fourier transform analysis showed that the electron-lucent bands present sub-bands with a regular pattern. An analysis of the costa fraction via one- and two-dimensional electrophoresis and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) allowed the identification of 54 hypothetical proteins. Fourteen of those proteins were considered to be major components of the fraction. CONCLUSIONS: The costa of T. foetus is a complex and organised cytoskeleton structure made of a large number of proteins which is assembled into filamentous structures. Some of these proteins exhibit uncharacterised domains and no function related according to gene ontology, suggesting that the costa structure may be formed by a new class of proteins that differ from those previously described in other organisms. Seven of these proteins contain prefoldin domains displaying coiled-coil regions. This propriety is shared with proteins of the striated fibres of other protozoan as well as in intermediate filaments. SIGNIFICANCE: Our observations suggest the presence of a new class of the cytoskeleton filaments in T. foetus. We believe that our data could auxiliate in determining the specific locations of these proteins in the distinct regions that compose the costa, as well as to define the functional roles of each component. Therefore, our study will help in the better understanding of the organisation and function of this structure in unicellular organisms.
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Citoesqueleto/química , Proteínas Protozoarias/química , Trichomonadida/metabolismo , Fraccionamiento Celular , Citoesqueleto/metabolismo , Citoesqueleto/ultraestructura , Microscopía Electrónica , Proteínas Protozoarias/metabolismo , Proteínas Protozoarias/ultraestructura , Espectrometría de Masas en Tándem , Trichomonadida/química , Trichomonadida/ultraestructuraRESUMEN
BACKGROUND: HLA genes are the most polymorphic of the human genome and have distinct allelic frequencies in populations of different geographical regions of the world, serving as genetic markers in ancestry studies. In addition, specific HLA alleles may be associated with various autoimmune and infectious diseases. The bone marrow donor registry in Brazil is the third largest in the world, and it counts with genetic typing of HLA-A, -B, and -DRB1. Since 1991 Brazil has maintained the DATASUS database, a system fed with epidemiological and health data from compulsory registration throughout the country. METHODS: In this work, we perform spatial analysis and georeferencing of HLA genetic data from more than 86,000 bone marrow donors from Rio Grande do Sul (RS) and data of hospitalization for rheumatoid arthritis, multiple sclerosis and Crohn's disease in RS, comprising the period from 1995 to 2016 obtained through the DATASUS system. The allele frequencies were georeferenced using Empirical Bayesian Kriging; the diseases prevalence were georeferenced using Inverse Distance Weighted and cluster analysis for both allele and disease were performed using Getis-Ord Gi* method. Spearman's test was used to test the correlation between each allele and disease. RESULTS: The results indicate a HLA genetic structure compatible with the history of RS colonization, where it is possible to observe differentiation between regions that underwent different colonization processes. Spatial analyzes of autoimmune disease hospitalization data were performed revealing clusters for different regions of the state for each disease analyzed. The correlation test between allelic frequency and the occurrence of autoimmune diseases indicated a significant correlation between the HLA-B*08 allele and rheumatoid arthritis. CONCLUSIONS: Genetic mapping of populations and the spatial analyzes such as those performed in this work have great economic relevance and can be very useful in the formulation of public health campaigns and policies, contributing to the planning and adjustment of clinical actions, as well as informing and educating professionals and the population.
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Enfermedades Autoinmunes/epidemiología , Enfermedades Autoinmunes/genética , Mapeo Cromosómico/métodos , Bases de Datos Genéticas , Antígenos HLA/genética , Análisis Espacial , Brasil/epidemiología , Mapeo Cromosómico/estadística & datos numéricos , Bases de Datos Genéticas/estadística & datos numéricos , HumanosRESUMEN
Water quality monitoring through remote sensing with UAVs is best conducted using multispectral sensors; however, these sensors are expensive. We aimed to predict multispectral bands from a low-cost sensor (R, G, B bands) using artificial neural networks (ANN). We studied a lake located on the campus of Unisinos University, Brazil, using a low-cost sensor mounted on a UAV. Simultaneously, we collected water samples during the UAV flight to determine total suspended solids (TSS) and dissolved organic matter (DOM). We correlated the three bands predicted with TSS and DOM. The results show that the ANN validation process predicted the three bands of the multispectral sensor using the three bands of the low-cost sensor with a low average error of 19%. The correlations with TSS and DOM resulted in R² values of greater than 0.60, consistent with literature values.
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Staphylococcus aureus bone and joint infection (BJI) is associated with significant rates of chronicity and relapse. In this study, we investigated how S. aureus is able to adapt to the human environment by comparing isolates from single patients with persisting or relapsing BJIs that were recovered during the initial and recurrent BJI episodes. In vitro and in vivo assays and whole-genome sequencing analyses revealed that the recurrent isolates induced a reduced inflammatory response, formed more biofilms, persisted longer in the intracellular compartments of host bone cells, were less cytotoxic and induced less mortality in a mouse infection model compared with the initial isolates despite the lack of significant changes at the genomic level. These findings suggest that S. aureus BJI chronicization is associated with an in vivo bacterial phenotypical adaptation that leads to decreased virulence and host immune escape, which is linked to increased intraosteoblastic persistence and biofilm formation.
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Artritis Infecciosa/microbiología , Biopelículas , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/fisiología , Adaptación Fisiológica , Adulto , Anciano de 80 o más Años , Secuencia de Aminoácidos , Células Cultivadas , Enfermedad Crónica , Progresión de la Enfermedad , Femenino , Proteínas Hemolisinas/metabolismo , Interacciones Huésped-Patógeno , Humanos , Masculino , Osteoblastos/inmunología , Osteoblastos/microbiologíaRESUMEN
In this study, we present a detailed family-level phylogenetic hypothesis for the largest avian order (Aves: Passeriformes) and an unmatched multi-calibrated, relaxed clock inference for the diversification of crown passerines. Extended taxon sampling allowed the recovery of many challenging clades and elucidated their position in the tree. Acanthisittia appear to have diverged from all other passerines at the early Paleogene, which is considerably later than previously suggested. Thus, Passeriformes may be younger and represent an even more intense adaptive radiation compared to the remaining avian orders. Based on our divergence time estimates, a novel hypothesis for the diversification of modern Suboscines is proposed. According to this hypothesis, the first split between New and Old World lineages would be related to the severing of the Africa-South America biotic connection during the mid-late Eocene, implying an African origin for modern Eurylaimides. The monophyletic status of groups not recovered by any subsequent study since their circumscription, viz. Sylvioidea including Paridae, Remizidae, Hyliotidae, and Stenostiridae; and Muscicapoidea including the waxwing assemblage (Bombycilloidea) were notable topological findings. We also propose possible ecological interactions that may have shaped the distinct Oscine distribution patterns in the New World. The insectivorous endemic Oscines of the Americas, Vireonidae (Corvoidea), Mimidae, and Troglodytidae (Muscicapoidea), probably interfered with autochthonous Suboscines through direct competition. Thus, the Early Miocene arrival of these lineages before any other Oscines may have occupied the few available niches left by Tyrannides, constraining the diversification of insectivorous Oscines that arrived in the Americas later. The predominantly frugivorous-nectarivorous members of Passeroidea, which account for most of the diversity of New World-endemic Oscines, may not have been subjected to competition with Tyrannides. In fact, the vast availability of frugivory niches combined with weak competition with the autochthonous passerine fauna may have been crucial for passeroids to thrive in the New World.
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Biodiversidad , Passeriformes/clasificación , Filogenia , Pájaros Cantores/clasificación , África , Animales , Evolución Biológica , Fenómenos Geológicos , Passeriformes/genética , Filogeografía , Análisis de Secuencia de ADN , Pájaros Cantores/genética , América del SurRESUMEN
OBJECTIVES: (1) To assess whether there is evidence of an association between the number of peri-implant tissue complications and patient characteristics such as gender, diabetes status, smoking status, and bite force; (2) To assess whether there is evidence of an association between the number of peri-implant tissue complications and location of the implant, surgical technique used, bone graft status and sinus lift status. MATERIALS AND METHODS: This randomized, controlled clinical trial included a total of 176 implants (OsseoSpeed, DENTSPLY) in 67 participants with 88 fixed dental prostheses. Information was obtained from health histories, a baseline exam, surgical notes, and post-operative exams. The data were analyzed using Fisher's exact and Mann-Whitney tests and generalized estimating equations using logistic regression with a significance level set at 0.05. RESULTS: All 176 implants survived within a recall period of 3 years, but 11 implants demonstrated peri-implant tissue complications. Ten sites showed dehiscence and one case exhibited vertical bone loss. There was a statistically significant association between surgical technique used (1-stage or 2-stage) and the presence of soft tissue complications (P = 0.005), where 2-stage surgery was associated with a higher frequency of peri-implant soft tissue complications. A correlation, although not statistically significant (P = 0.077), was noted, between peri-implant tissue complications and bone grafting, suggesting a possible role for this factor as well. CONCLUSIONS: Participants who did not require any second-stage surgery at the implant sites experienced fewer complications. Therefore, additional surgical procedures should be performed judiciously considering their possible effects on peri-implant tissue health. CLINICAL SIGNIFICANCE: The clinical implication of this research study is that secondary surgery should be considered with caution during implant placement and it should be performed only when other options have been exhausted, as it has been shown to have a direct adverse effect on the long-term peri-implant tissue health.
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Complicaciones Posoperatorias/epidemiología , Adulto , Anciano , Trasplante Óseo , Implantación Dental Endoósea/métodos , Implantes Dentales , Prótesis Dental de Soporte Implantado , Fracaso de la Restauración Dental , Complicaciones de la Diabetes , Femenino , Humanos , Masculino , Persona de Mediana Edad , Factores de Riesgo , Factores Sexuales , Elevación del Piso del Seno Maxilar , Fumar/efectos adversos , Resultado del TratamientoRESUMEN
Tritrichomonas foetus is a protist that causes bovine trichomoniasis and presents a well-developed Golgi. There are very few studies concerning the Golgi in trichomonads. In this work, monoclonal antibodies were raised against Golgi of T. foetus and used as a tool on morphologic and biochemical studies of this organelle. Among the antibodies produced, one was named mAb anti-Golgi 20.3, which recognized specifically the Golgi complex by fluorescence and electron microscopy. By immunoblotting this antibody recognized two proteins with 60 and 66 kDa that were identified as putative beta-tubulin and adenosine triphosphatase, respectively. The mAb 20.3 also recognized the Golgi complex of the Trichomonas vaginalis, a human parasite. In addition, the nucleotide coding sequences of these proteins were identified and included in the T. foetus database, and the 3D structure of the proteins was predicted. In conclusion, this study indicated: (1) adenosine triphosphatase is present in the Golgi, (2) ATPase is conserved between T. foetus and T. vaginalis, (3) there is new information concerning the nucleic acid sequences and protein structures of adenosine triphosphatase and beta-tubulin from T. foetus and (4) the mAb anti-Golgi 20.3 is a good Golgi marker and can be used in future studies.
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Adenosina Trifosfatasas/metabolismo , Anticuerpos Antiprotozoarios/inmunología , Antígenos de Protozoos/inmunología , Aparato de Golgi/ultraestructura , Infecciones Protozoarias en Animales/parasitología , Tritrichomonas foetus/ultraestructura , Adenosina Trifosfatasas/química , Adenosina Trifosfatasas/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales/inmunología , Secuencia de Bases , Bovinos , Femenino , Aparato de Golgi/química , Aparato de Golgi/metabolismo , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica de Transmisión/veterinaria , Microscopía Fluorescente/veterinaria , Modelos Moleculares , Proteínas Protozoarias/química , Proteínas Protozoarias/inmunología , Proteínas Protozoarias/metabolismo , Alineación de Secuencia/veterinaria , Análisis de Secuencia de ADN/veterinaria , Trichomonas vaginalis/enzimología , Trichomonas vaginalis/inmunología , Tritrichomonas foetus/enzimología , Tritrichomonas foetus/genética , Tritrichomonas foetus/inmunologíaRESUMEN
The present study aimed to develop and implement a method for detection and classification of spectral signatures in point clouds obtained from terrestrial laser scanner in order to identify the presence of different rocks in outcrops and to generate a digital outcrop model. To achieve this objective, a software based on cluster analysis was created, named K-Clouds. This software was developed through a partnership between UNISINOS and the company V3D. This tool was designed to begin with an analysis and interpretation of a histogram from a point cloud of the outcrop and subsequently indication of a number of classes provided by the user, to process the intensity return values. This classified information can then be interpreted by geologists, to provide a better understanding and identification from the existing rocks in the outcrop. Beyond the detection of different rocks, this work was able to detect small changes in the physical-chemical characteristics of the rocks, as they were caused by weathering or compositional changes.
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Rayos Láser , Modelos Teóricos , Tecnología de Sensores Remotos , Programas Informáticos , Brasil , Análisis por Conglomerados , Procesamiento de Imagen Asistido por ComputadorRESUMEN
Russet-mantled Foliage-gleaner is an extremely poorly known species, the range of which is centered in the Brazilian Cerrado, where it inhabits riparian forests. Two subspecies are recognized, but the limits of their ranges are controversial. Furthermore, it was recently suggested that the species is one of the few in the family Furnariidae to show sexual dichromatism. In this paper we examined the plumage coloration and morphometrics of 33 study skins (85% of the available specimens). We conclude that the geographic variation and sexual dichromatism reported for S. dimidiata originated from misinterpretation of the plumage variation observed in this species, which is best considered monotypic. We also present natural history data on Russet-mantled Foliage-gleaners and suggest considering it a globally Vulnerable species.