Molecular analysis of maize (Zea mays L.)-infecting mastreviruses in Ethiopia reveals marked diversity of virus genomes and a novel species.

Maize (Zea mays L.) is host for more than 50 virus species worldwide with Maize streak virus (MSV) (genus Mastrevirus) causing significant yield losses in Africa. A survey for viruses infecting maize was conducted in major growing regions of Ethiopia. To test for DNA viruses, in particular mastreviruses, rolling circle amplification was performed for the analysis of virus composition in assayed samples. Following the analysis of the entire virus genomes, three genetic groups, each representing distinct virus species, were identified. The first group was almost identical with the A-strain of MSV. The next sequence-cluster shared 96-98% identity with isolates of Maize streak reunion virus (MSRV) confirming the presence of this virus also in continental East Africa. Sequence analysis of additional virus genomes (each 2846 nt) in length revealed only a limited 70-71% nt identity with MSRV isolates and an even lower identity (< 64%) with sequences of mastreviruses described elsewhere. Our analysis suggests a novel virus species, which is tentatively named maize streak dwarfing virus (MSDV). The pairwise comparison of capsid protein and replication-associated protein (Rep) of the novel species revealed a limited identity of 63% and 68% with the respective protein sequences of MSRV. The incidence of the virus species in the maize regions of Ethiopia was studied across 89 samples collected during four growing seasons. PCR analysis with general and specific mastrevirus primers showed that MSV is the most incident virus (39.3%) followed by MSRV (14.6%) and MSDV (12.4%). Identification of three different mastrevirus species in a confined geographical location on the same host, maize, is unprecedented, and suggests that Ethiopia may be one of the potential hot spots for diversity of maize-infecting mastreviruses.

Correction to: Molecular analysis of maize (Zea mays L.)-infecting mastreviruses in Ethiopia reveals marked diversity of virus genomes and a novel species.

The original version of this article unfortunately contained errors in the "Results" section.

Serological, biological and molecular characterization of viruses causing mosaic diseases on cabbage (Brassica sp L.) in Central Ethiopia.

The productivity of cabbage (Brassica oleracea var. capitata) in Ethiopia has been generally low due to several biotic and abiotic constraints among which are several viral diseases. There is a recent report indicating that this economically important vegetable is seriously affected in Ethiopia by cauliflower mosaic virus (CaMV) and turnip mosaic virus (TuMV). However, little information exists on the incidence and distribution of these viruses as the previous report is based on samples only from Addis Ababa. In this study, a total of 370 leaf samples were collected from 75 cabbage growing fields in Central Ethiopia in two rounds of survey. Two cabbage varieties locally known as "Habesha gomen" and "Tikur gomen" with virus-like symptoms were collected and tested with Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) using polyclonal antibodies specific to CaMV and TuMV. Results from serological diagnosis were confirmed with PCR and Sanger sequencing. The results indicated a high incidence and wide distribution of both viruses in Central Ethiopia with an average of 29.5% infection for CaMV and 40% for TuMV. Biological inoculation tests for CaMV or TuMV or both on healthy cabbage seedlings gave similar symptoms as those observed in the field. Symptom severity was higher with co-infection of CaMV and TuMV followed by TuMV single infection. BLAST analysis showed that TuMV and CaMV isolates from Ethiopia have nucleotide identity of 95-98% and 93-98%, respectively to previously reported isolates. Phylogenetic analysis revealed that CaMV isolates from Ethiopia are closely related to isolates from USA and Italy within Group II clade whereas TuMV isolates have close similarities with isolates from World B clade including isolates from Kenya, UK, Japan and the Netherlands. The identification of the causative agents of the mosaic disease observed on cabbage in Central Ethiopia may lay the foundation for future management studies.