p-Smad3 differentially regulates the cytological behavior of osteoclasts before and after osteoblasts maturation.

BACKGROUND: A series of previous investigations have revealed that p-Smad3 plays a facilitative role in the differentiation and maturation of osteoblasts, while also regulating the expression of certain intercellular communication factors. However, the effects of p-Smad3 in osteoblasts before and after maturation on the proliferation, migration, differentiation, apoptosis and other cellular behaviors of osteoclasts have not been reported. METHODS: MC3T3-E1 cells were cultured in osteogenic induction medium for varying durations, After that, the corresponding conditioned medium was collected and the osteoclast lineage cells were treated. To elucidate the regulatory role of p-Smad3 within osteoblasts, we applied the activator TGF-ß1 and inhibitor SIS3 to immature and mature osteoblasts and collected corresponding conditioned media for osteoclast intervention. RESULTS: We observed an elevation of p-Smad3 and Smad3 during the early stage of osteoblast differentiation, followed by a decline in the later stage. we discovered that as osteoblasts mature, their conditioned media inhibit osteoclasts differentiation and the osteoclast-coupled osteogenic effect. However, it promotes apoptosis in osteoclasts and the angiogenesis coupled with osteoclasts. p-Smad3 in immature osteoblasts, through paracrine effects, promotes the migration, differentiation, and osteoclast-coupled osteogenic effects of osteoclast lineage cells. For mature osteoblasts, p-Smad3 facilitates osteoclast apoptosis and the angiogenesis coupled with osteoclasts. CONCLUSIONS: As pre-osteoblasts undergo maturation, p-Smad3 mediated a paracrine effect that transitions osteoclast cellular behaviors from inducing differentiation and stimulating bone formation to promoting apoptosis and coupling angiogenesis.

Impact Assessment of heavy metal cations to the characteristics of photosynthetic phycocyanin.

This work has assessed the impact of typical heavy metal cations on C-phycocyanin in vitro and in silico. At low concentrations (<2×10-6 mol/L), the influence of Pb2+ is the highest on the light absorption of C-phycocyanin trimer. At higher concentrations, however, a new order of influence on the light absorption has been observed with Cd2+ < Cu2+ < Pb2+ < Zn2+. The fluorescence polarization has changed from the order of Cd2+ < Pb2+≈Cu2+ < Zn2+ to Cd2+ < Cu2+ < Pb2+ < Zn2+, when the metal concentrations reaches 2×10-6 mol/L. The mechanisms for these findings have been studied using FTIR, hydrophobic probe, isothermal titration calorimetry and molecular docking for the analysis of structure disorder of C-phycocyanin. It has been suggested that the secondary structure of C-phycocyanin affects more to the light absorbance while the fluorescence characteristics relies more on the tertiary structure. The interaction between Pb2+ and C-phycocyanin is both enthalpically and entropically favoured, whereas the interactions for Cd2+, Cu2+ and Zn2+ are entropically driven. The ion-molecular docking suggests that the structure disorder of C-phycocyanin relies on the molecular interactions with metal ions. The in silico study also showed that the binding cites of Zn2+ are closer to chromophores.