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AKT2 potassium (K+) channels are members of the plant Shaker family which mediate dual-directional K+ transport with weak voltage-dependency. Here we show that OsAKT2 of rice (Oryza sativa) functions mainly as an inward rectifier with strong voltage-dependency and acutely suppressed outward activity. This is attributed to the presence of a unique K191 residue in the S4 domain. The typical bi-directional leak-like property was restored by a single K191R mutation, indicating that this functional distinction is an intrinsic characteristic of OsAKT2. Furthermore, the opposite R195K mutation of AtAKT2 changed the channel to an inward-rectifier similar to OsAKT2. OsAKT2 was modulated by OsCBL1/OsCIPK23, evoking the outward activity and diminishing the inward current. The physiological relevance in relation to the rectification diversity of OsAKT2 was addressed by functional assembly in the Arabidopsis (Arabidopsis thaliana) akt2 mutant. Overexpression (OE) of OsAKT2 complemented the K+ deficiency in the phloem sap and leaves of the mutant plants but did not significantly contribute to the transport of sugars. However, the expression of OsAKT2-K191R overcame both the shortage of phloem K+ and sucrose of the akt2 mutant, which was comparable to the effects of the OE of AtAKT2, while the expression of the inward mutation AtAKT2-R195K resembled the effects of OsAKT2. Additionally, OE of OsAKT2 ameliorated the salt tolerance of Arabidopsis.
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Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Oryza/genética , Oryza/metabolismo , Floema/metabolismo , Canales de Potasio/metabolismo , Tolerancia a la Sal/fisiología , Productos Agrícolas/genética , Productos Agrícolas/metabolismo , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Mutación , Floema/genética , Canales de Potasio/genética , Tolerancia a la Sal/genéticaRESUMEN
BACKGROUND: To explore if the quantitative perfusion histogram parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) correlates with the expression of PTEN, P-Akt and m-TOR protein in lung cancer. METHODS: Thirty-three patients with 33 lesions who had been diagnosed with lung cancer were enrolled in this study. They were divided into three groups: squamous cell carcinoma (SCC, 15 cases), adenocarcinoma (AC, 12 cases) and small cell lung cancer (SCLC, 6 cases). Preoperative imaging (conventional imaging and DCE-MRI) was performed on all patients. The Exchange model was used to measure the phar- macokinetic parameters, including Ktrans, Vp, Kep, Ve and Fp, and then the histogram parameters meanvalue, skewness, kurtosis, uniformity, energy, entropy, quantile of above five parameters were analyzed. The expression of PTEN, P-Akt and m-TOR were assessed by immunohistochemistry. Spearman correlation analysis was used to compare the correlation between the quantitative perfusion histogram parameters and the expression of PTEN, P-Akt and m-TOR in different pathological subtypes of lung cancer. RESULTS: The expression of m-TOR (P = 0.013) and P-Akt (P = 0.002) in AC was significantly higher than those in SCC. Vp (uniformity) in SCC group, Ktrans (uniformity), Ve (kurtosis, Q10, Q25) in AC group, Fp (skewness, kurtosis, energy), Ve (Q75, Q90, Q95) in SCLC group was positively correlated with PTEN, and Fp (entropy) in the SCLC group was negatively correlated with PTEN (P < 0.05); Kep (Q5, Q10) in the SCLC group was positively correlated with P-Akt, and Kep (energy) in the SCLC group was negatively correlated with P-Akt (P < 0.05); Kep (Q5) in SCC group and Vp (meanvalue, Q75, Q90, Q95) in SCLC group was positively correlated with m-TOR, and Ve (meanvalue) in SCC group was negatively correlated with m-TOR (P < 0.05). CONCLUSIONS: The quantitative perfusion histogram parameters of DCE-MRI was correlated with the expression of PTEN, P-Akt and m-TOR in different pathological types of lung cancer, which may be used to indirectly evaluate the activation status of PI3K/Akt/mTOR signal pathway gene in lung cancer, and provide important reference for clinical treatment.
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Adenocarcinoma/diagnóstico por imagen , Carcinoma de Células Escamosas/diagnóstico por imagen , Neoplasias Pulmonares/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Carcinoma Pulmonar de Células Pequeñas/diagnóstico por imagen , Adenocarcinoma/metabolismo , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/metabolismo , Medios de Contraste , Femenino , Humanos , Neoplasias Pulmonares/metabolismo , Masculino , Persona de Mediana Edad , Proteínas de Neoplasias/metabolismo , Fosfohidrolasa PTEN/metabolismo , Imagen de Perfusión/métodos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Carcinoma Pulmonar de Células Pequeñas/metabolismo , Serina-Treonina Quinasas TOR/metabolismoRESUMEN
PURPOSE: To compare prethoracoscopy localization of small pulmonary nodules (SPNs) by means of medical adhesive versus hookwire. MATERIALS AND METHODS: One hundred seven patients who underwent video-assisted thoracoscopic surgery resection for SPNs were consecutively recruited in this retrospective cohort study. Patients were divided into 2 groups according to the material used for localization of the SPNs: the medical adhesive group (n = 88) and the hookwire group (n = 19). The baseline data were collected, and operation waiting time (OWT; the time gap between localization and surgery), wedge resection performing time (WRPT), pathologic result, and complications of the 2 groups were assessed. RESULTS: All SPNs were successfully marked. No differences in pathologic result (P = .676), wedge resection, or segmentectomy rate (P = .679) were observed. OWT was markedly longer in the medical adhesive group than in the hookwire group (P < .001), whereas WRPT was similar in the 2 groups (P = .926). There were significantly (P = .004) fewer complications in the medical adhesive group (37.42%) than in the hookwire group (15.79%). Regarding individual complications, hemorrhage occurred significantly less in the medical adhesive group than in the hookwire group (9% vs 68%; P < .001), and no differences of cough, pneumothorax, or chest pain were found between the 2 groups (all P > .05). Multivariate logistic regression analysis further validated that hookwire was independently correlated with a higher risk of complication occurrence (P = .008) and hemorrhage occurrence (P < .001) compared with medical adhesive. CONCLUSIONS: Compared with hookwire, localization via medical adhesive can achieve a flexible time gap between localization and surgery. It also decreases the complication rate and increases convenience owing to no need for an anchor hook.
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Neoplasias Pulmonares/diagnóstico por imagen , Nódulos Pulmonares Múltiples/diagnóstico por imagen , Neumonectomía/métodos , Nódulo Pulmonar Solitario/diagnóstico por imagen , Cirugía Torácica Asistida por Video , Adhesivos Tisulares/administración & dosificación , Tomografía Computarizada por Rayos X/instrumentación , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Neoplasias Pulmonares/patología , Neoplasias Pulmonares/cirugía , Masculino , Persona de Mediana Edad , Nódulos Pulmonares Múltiples/patología , Nódulos Pulmonares Múltiples/cirugía , Neumonectomía/efectos adversos , Complicaciones Posoperatorias/etiología , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Nódulo Pulmonar Solitario/patología , Nódulo Pulmonar Solitario/cirugía , Cirugía Torácica Asistida por Video/efectos adversos , Factores de Tiempo , Adhesivos Tisulares/efectos adversos , Resultado del Tratamiento , Carga Tumoral , Adulto JovenRESUMEN
Efficient stomatal opening requires activation of KAT-type K(+) channels, which mediate K(+) influx into guard cells. Most KAT-type channels are functionally facilitated by extracellular acidification. However, despite sequence and structural homologies, the maize counterpart of Arabidopsis KAT1 (ZmK2.1) is resistant to pH activation. To understand the structural determinant that results in the differential pH activation of these counterparts, we analysed chimeric channels and channels with point mutations for ZmK2.1 and its closest Arabidopsis homologue KAT1. Exchange of the S1-S2 linkers altered the pH sensitivity between the two channels, suggesting that the S1-S2 linker is essentially involved in the pH sensitivity. The effects of D92 mutation within the linker motif together with substitution of the first half of the linker largely resemble the effects of substitution of the complete linker. Topological modelling predicts that one of the two cysteines located on the outer face section of the S5 domain may serve as a potential titratable group that interacts with the S1-S2 linker. The difference between ZmK2.1 and KAT1 is predicted to be the result of the distance of the stabilized linkers from the titratable group. In KAT1, residue K85 within the linker forms a hydrogen bond with C211 that enables the pH activation; conversely, the linker of ZmK2.1 is distantly located and thus does not interact with the equivalent titration group (C208). Thus, in addition to the known structural contributors to the proton activation of KAT channels, we have uncovered a previously unidentified component that is strongly involved in this complex proton activation network.
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Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Plantas/genética , Canales de Potasio de Rectificación Interna/genética , Zea mays/genética , Secuencia de Aminoácidos , Aminoácidos/química , Aminoácidos/genética , Aminoácidos/metabolismo , Arabidopsis/metabolismo , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Sitios de Unión/genética , Enlace de Hidrógeno , Concentración de Iones de Hidrógeno , Modelos Moleculares , Proteínas Mutantes Quiméricas/química , Proteínas Mutantes Quiméricas/genética , Proteínas Mutantes Quiméricas/metabolismo , Mutación , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Canales de Potasio de Rectificación Interna/química , Canales de Potasio de Rectificación Interna/metabolismo , Unión Proteica , Dominios Proteicos , Homología de Secuencia de Aminoácido , Zea mays/metabolismoRESUMEN
A novel rapid synthesis of quinolines from 2-alkenylanilines has been described; the reaction involves an unexpected DMAP-catalyzed cyclization of 2-alkenylanilines with di-tert-butyl dicarbonate (Boc2O, 2.0 equiv), and a series of tert-butyl quinolin-2-yl carbonate with various functional groups have been synthesized in good yields under mild conditions. Furthermore, the tert-butyl quinolin-2-yl carbonate can be easily converted into corresponding quinolinones and 2-(pseudo)haloquinolines.
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A novel rapid synthesis of indoles from N-Ts-2-alkenylanilines has been described; the reaction involves a NIS-mediated cascade C-N bond formation/aromatization, and a series of indoles with various functional groups have been synthesized in good to excellent yields under mild conditions without any other additives or catalysts.
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Compuestos de Anilina/química , Indoles/síntesis química , Metales/química , Catálisis , Indoles/química , Estructura MolecularRESUMEN
Lymphoepithelioma-like intrahepatic cholangiocarcinoma (LEL-ICC) is a rare distinctive variant of liver cancer with unique epidemiological and pathological characteristics, including dense lymphocyte infiltration. We herein describe a 67-year-old Chinese man with LEL-ICC. The patient had undergone endoscopic extraction of a bile duct stone 1 month prior. Contrast-enhanced abdominal computed tomography (CT) revealed a 2.5- × 2.5- × 1.5-cm low-density mass located in a covert part of the left lateral segment of the liver. Contrast-enhanced magnetic resonance imaging revealed a hyperintense lesion on T2-weighted and diffusion-weighted images of the left lateral liver, with similar size and signal characteristics in the arterial and portal venous phases. The patient subsequently underwent left lateral laparoscopic hepatectomy. The results of postoperative pathology and immunohistochemistry allowed for the definitive diagnosis. In situ hybridization using an Epstein-Barr virus-encoded RNA probe revealed extensive reactivity in the tumor cell nuclei, supporting a diagnosis of LEL-ICC. The patient was recurrence-free at 12 months postoperatively as shown by CT. A literature review indicated that in middle-aged patients with Epstein-Barr virus infection, a liver mass with a well-defined margin and a combination of hypervascularity and delayed intratumoral enhancement on CT and magnetic resonance imaging may suggest a diagnosis of LEL-ICC.
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Neoplasias de los Conductos Biliares , Carcinoma de Células Escamosas , Colangiocarcinoma , Infecciones por Virus de Epstein-Barr , Neoplasias Hepáticas , Masculino , Persona de Mediana Edad , Humanos , Anciano , Infecciones por Virus de Epstein-Barr/diagnóstico por imagen , Herpesvirus Humano 4 , Colangiocarcinoma/diagnóstico por imagen , Colangiocarcinoma/cirugía , Neoplasias Hepáticas/diagnóstico por imagen , Neoplasias Hepáticas/cirugía , Carcinoma de Células Escamosas/patología , Neoplasias de los Conductos Biliares/diagnóstico por imagen , Neoplasias de los Conductos Biliares/cirugía , Conductos Biliares Intrahepáticos/diagnóstico por imagen , Conductos Biliares Intrahepáticos/cirugía , Conductos Biliares Intrahepáticos/patologíaRESUMEN
Purpose: To evaluate the effectiveness of machine learning model based on magnetic resonance imaging (MRI) in identifying microsatellite instability (MSI) status and PD-L1 expression in endometrial cancer (EC). Methods: This retrospective study included 82 EC patients from 2 independent centers. Radiomics features from the intratumoral and peritumoral regions, obtained from four conventional MRI sequences (T2-weighted images; contrast-enhanced T1-weighted images; diffusion-weighted images; apparent diffusion coefficient), were combined with clinicopathologic characteristics to develop machine learning model for predicting MSI status and PD-L1 expression. 60 patients from center 1 were used as the training set for model construction, while 22 patients from center 2 were used as an external validation set for model evaluation. Results: For predicting MSI status, the clinicopathologic model, radscore model, and combination model achieved area under the curves (AUCs) of 0.728, 0.833, and 0.889 in the training set, respectively, and 0.595, 0.790, and 0.848 in the validation set, respectively. For predicting PD-L1 expression, the clinicopathologic model, radscore model, and combination model achieved AUCs of 0.648, 0.814, and 0.834 in the training set, respectively, and 0.660, 0.708, and 0.764 in the validation set, respectively. Calibration curve analysis and decision curve analysis demonstrated good calibration and clinical utility of the combination model. Conclusion: The machine learning model incorporating MRI-based radiomics features and clinicopathologic characteristics could be a potential tool for predicting MSI status and PD-L1 expression in EC. This approach may contribute to precision medicine for EC patients.
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The inflammatory response to viral infection is an important component of the antiviral response, a process that involves the activation and proliferation of CD8+ T, CD4+ T, and dendritic cells; thus, viral infection disrupts the immune homeostasis of the organism, leading to an increased release of inflammatory factors. Kikuchi-Fujimoto disease (KFD) is an inflammatory self-limited disorder of unknown etiology, and it is generally believed that the pathogenesis of this disease includes two aspects: viral infection and autoimmune response. Various immune cells, such as CD8+ T lymphocytes, CD4+ T lymphocytes, and CD123+ plasmacytoid dendritic cells, as well as the cytokines they induce and secrete, such as interferons, interleukins, and tumor necrosis factors, play a crucial role in the pathogenesis of KFD. In this article, we present a case study of a young female patient from China who exhibited typical symptoms of lymph node inflammation and fever. The diagnosis of KFD was confirmed through a lymph node biopsy. She presented with elevated ESR, IL-6, and IFN-γ. Viral markers showed elevated IgG and IgM of cytomegalovirus (CMV) and elevated IgG of Epstein-Barr virus (EBV), while changes occurred in the CD4+ T and CD8+ T cell counts. Eventually, the patient achieved disease relief through steroid treatment. Based on these findings, we conducted a comprehensive review of the involvement of viral infection-induced inflammatory response processes and autoimmunity in the pathogenesis of Kikuchi-Fujimoto disease.
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Infecciones por Virus de Epstein-Barr , Linfadenitis Necrotizante Histiocítica , Linfadenopatía , Fiebre Recurrente , Femenino , Humanos , Diagnóstico Diferencial , Infecciones por Virus de Epstein-Barr/patología , Herpesvirus Humano 4 , Linfadenitis Necrotizante Histiocítica/diagnóstico , Linfadenitis Necrotizante Histiocítica/tratamiento farmacológico , Linfadenitis Necrotizante Histiocítica/patología , Inmunoglobulina G , Ganglios Linfáticos/patología , Linfadenopatía/diagnósticoRESUMEN
To explore the hydrogen and oxygen stable isotopes and vapor sources of atmospheric precipitation in the loess region of north Shaanxi, we collected 107 samples of precipitation during 2018 to 2020 in the Dingbian County, Shaanxi Province. After measuring δ18O and δ2H of precipitation, we analyzed the isotopic characteristics and vapor sources of precipitation in different seasons. There was obvious seasonal variability in δ18O and δ2H, in that they were more depleted in the wet season (June-September) but more enriched in the dry season (April-May, October-November). In addition, the deuterium excess values were high in the dry season but low in the wet season. The local meteoric water line was regressed as δ2H=7.35δ18O+4.19 (R2=0.96, P<0.01) with slope and intercept lower than that of the global meteoric water line, indicating an impact of evaporation on isotope fractionation. There was temperature effect in the precipitation isotopes for the whole year and for the dry season, while the precipitation isotopes in the wet season were affected by the combined effects of temperature and rainfall amount. The HYSPLIT model showed that the dry season water vapor mainly came from the Atlantic Ocean and the polar Arctic Ocean, while the wet season vapor mainly came from the Indian Ocean and Pacific subject to the Westerly.
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Monitoreo del Ambiente , Lluvia , China , Isótopos de Oxígeno/análisis , Estaciones del AñoRESUMEN
The situations are complex and variant in the three stages of "carbon emission peak", "rapid reduction of carbon emission" and "deep decarbonization for carbon neutrality" in China's carbon neutralization roadmap. Forest carbon sequestration is an important means to achieve the goal of carbon neutralization in China. Its intertemporal allocation is a vital way to balance industrial emission reduction and forest carbon sequestration, reduce the cost of carbon neutrality, and gradually achieve the goal of carbon neutrality based on optimal cost. Based on the cost optimization allocation theory, we simulated the cost change process of three stages of carbon neutralization in China by quoting the theory of marginal carbon sequestration cost and combining with the existing domestic marginal abatement cost theory. The results showed that annual forest carbon sequestrations with the optimal cost in China was 20 million t, 775 million t and 1.982 billion t respectively in the three stages of "carbon emission peak", "rapid reduction of carbon emission" and "deep decarbonization for carbon neutrality", accounting for 1.8%, 17.5%, and 37.6% of the total emission reduction in each period. Compared with the way relying only on industrial emission reduction, forest carbon sequestration under the optimal cost design reduced the total cost by 48, 79136, and 909253 million US$ in the three stages of carbon neutralization, respectively. Due to the limited cost advantage of forest carbon sequestration, industrial emission reduction should be emphasized in the "carbon emission peak" stage. In the "rapid reduction of carbon emissions" stage, the cost advantage of forest carbon sequestration will be increasingly prominent. In the stage of "deep decarbonization for carbon neutrality", it is necessary to fully exploit the cost advantage of forest carbon sequestration to achieve the goal of "zero carbon" to avoid the risk of high costs, especially for industries with high decarbonization cost or that will never be completely decarbonized. The optimal cost design for forest carbon sequestration can save 988.437 billion US $ in carbon-neutral costs.
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Secuestro de Carbono , Gases de Efecto Invernadero , Carbono/análisis , Dióxido de Carbono/análisis , China , BosquesRESUMEN
Enhanced carotenoid accumulation in plants is crucial for the nutritional and health demands of the human body since these beneficial substances are acquired through dietary intake. Plastids are the major organelles to accumulate carotenoids in plants and it is reported that manipulation of a single plastid phosphate transporter gene enhances carotenoid accumulation. Amongst all phosphate transport proteins including phosphate transporters (PHTs), plastidial phosphate translocators (pPTs), PHOSPHATE1 (PHO1), vacuolar phosphate efflux transporter (VPE), and Sulfate transporter [SULTR]-like phosphorus distribution transporter (SPDT) in plants, plastidic PHTs (PHT2 & PHT4) are found as the only clade that is plastid located, and manipulation of which affects carotenoid accumulation. Manipulation of a single chromoplast PHT (PHT4;2) enhances carotenoid accumulation, whereas manipulation of a single chloroplast PHT has no impact on carotenoid accumulation. The underlying mechanism is mainly attributed to their different effects on plastid orthophosphate (Pi) concentration. PHT4;2 is the only chromoplast Pi efflux transporter, and manipulating this single chromoplast PHT significantly regulates chromoplast Pi concentration. This variation subsequently modulates the carotenoid accumulation by affecting the supply of glyceraldehyde 3-phosphate, a substrate for carotenoid biosynthesis, by modulating the transcript abundances of carotenoid biosynthesis limited enzyme genes, and by regulating chromoplast biogenesis (facilitating carotenoid storage). However, at least five orthophosphate influx PHTs are identified in the chloroplast, and manipulating one of the five does not substantially modulate the chloroplast Pi concentration in a long term due to their functional redundancy. This stable chloroplast Pi concentration upon one chloroplast PHT absence, therefore, is unable to modulate Pi-involved carotenoid accumulation processes and finally does affect carotenoid accumulation in photosynthetic tissues. Despite these advances, several cases including the precise location of plastid PHTs, the phosphate transport direction mediated by these plastid PHTs, the plastid PHTs participating in carotenoid accumulation signal pathway, the potential roles of these plastid PHTs in leaf carotenoid accumulation, and the roles of these plastid PHTs in other secondary metabolites are waiting for further research. The clarification of the above-mentioned cases is beneficial for breeding high-carotenoid accumulation plants (either in photosynthetic or non-photosynthetic edible parts of plants) through the gene engineering of these transporters.
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BACKGROUND: Cerebral small vessel disease (SVD) related brain changes have been found associated with various clinical symptoms of Parkinson disease (PD). On the other hand, PD pathology and treatment may also accelerate SVD progression. OBJECTIVE: The aim of this study is to explore the interplay between SVD and PD pathology using longitudinal dataset. METHODS: We screened 66 healthy controls (HCs) and 114 patients from the Parkinson Progression Markers Initiative (PPMI) database. The peak width of skeletonized mean diffusivity (PSMD) was quantified from diffusion tensor images to reflect vascular pathologies at baseline and 24 months follow-up, and dopamine transporter (DAT) imaging data was used to represent the extent of dopaminergic neuronal degeneration at the same point time. We compared the PSMD between PD patients and HCs, and analyzed whether PSMD and DAT availability could predict each other's progression using multiple regression analyses in PD patients. RESULTS: PSMD at baseline had no significant difference between the HCs and patients with PD (P = 0.169). Higher baseline PSMD was associated with less DAT reduction in the caudate (ß = 0.216, P = 0.029), but not the putamen (ß = 0.058, P = 0.552) in PD patients. Baseline caudate and putamen DAT availability had no significant association with PSMD progression (ß = -0.006, P = 0.950; ß = 0.017, P = 0.860, respectively). CONCLUSIONS: Mild SVD might slow down PD pathology progression, while the effect of PD pathology on the progression of SVD was not significant.
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Enfermedades de los Pequeños Vasos Cerebrales , Enfermedad de Parkinson , Encéfalo/diagnóstico por imagen , Enfermedades de los Pequeños Vasos Cerebrales/complicaciones , Enfermedades de los Pequeños Vasos Cerebrales/diagnóstico por imagen , Imagen de Difusión por Resonancia Magnética , Humanos , Estudios Longitudinales , Enfermedad de Parkinson/complicaciones , Enfermedad de Parkinson/diagnóstico por imagen , Tomografía Computarizada de Emisión de Fotón ÚnicoRESUMEN
Reactive lymphoid hyperplasia (RLH) of the liver is a rare benign disease. This article describes a 77-year-old female patient with RLH of the liver. The patient was admitted to the hospital due to atrial fibrillation. A liver tumor was incidentally found during abdominal enhanced CT. Further magnetic resonance imaging (MRI) and PET/CT showed four lesions in the liver. The imaging findings suggested hepatocellular carcinoma (HCC), but it was not consistent that the patient had no history of liver cirrhosis and hepatitis, and a variety of tumor markers were within the normal range. The largest lesion was surgically removed and microscopically diagnosed as RLH of the liver. The pathology included a large number of reactive hyperplastic lymphoid follicles. Immunohistochemical examination showed that the infiltrating lymphocytes were polyclonal. The authors believe that the perinodular enhancement on MRI, the obvious limitation of diffusion on DWI, the insignificant increase of SUVmax on PET-CT delayed phase, and the support of clinical data can help distinguish liver RLH from lymphoma and HCC.
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Maize grows in nitrate-dominated dryland soils, but shortly upon localized dressing of nitrogen fertilizers, ammonium is retained as a noticeable form of nitrogen source available to roots. Thus in addition to nitrate, the absorption of ammonium can be an important strategy that promotes rapid plant growth at strong nitrogen demanding stages. The present study reports the functional characterization of three root-expressed ammonium transporters (AMTs), aiming at finding out functional and regulatory properties that correlate with efficient nitrogen acquisition of maize. Using a stable electrophysiological recording method we previously established in Xenopus laevis oocytes that integrates the capture of currents in response to voltage ramps with onsite stability controls, we demonstrate that all three ZmAMT1s engage NH4 + uniporting as ammonium uptake mechanisms. The K m value for ZmAMT1.1a, 1.1b, or ZmAMT1.3 is, respectively, 9.9, 15.6, or 18.6 µM, indicating a typical high-affinity transport of NH4 + ions. Importantly, the uptake currents of these ZmAMT1s are markedly amplified upon extracellular acidification. A pH drop from 7.4 to 5.4 results in a 140.8%, 64.1% or a 120.7% increase of ammonium uptake activity through ZmAMT1.1a, 1.1b, or ZmAMT1.3. Since ammonium uptake by plant roots accompanies a spontaneous acidification to the root medium, the functional promotion of ZmAMT1.1a, 1.1b, and ZmAMT1.3 by low pH is in line with the facilitated ammonium uptake activity in maize roots. Furthermore, the expression of the three ZmAMT1 genes is induced under ammonium-dominated conditions. Thus we describe a facilitated ammonium uptake strategy in maize roots by functional and expression regulations of ZmAMT1 transporters that may coordinate with efficient acquisition of this form of nitrogen source when available.
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AtAMT1;3 is a major contributor to high-affinity ammonium uptake in Arabidopsis roots. Using a stable electrophysiological recording strategy, we demonstrate in Xenopus laevis oocytes that AtAMT1;3 functions as a typical high-affinity NH4 + uniporter independent of protons and Ca2+. The findings that AtAMT1;3 transports methylammonium (MeA+, a chemical analog of NH4 +) with extremely low affinity (K m in the range of 2.9-6.1 mM) led to investigate the mechanisms underlying substrate binding. Homologous modeling and substrate docking analyses predicted that the deduced substrate binding motif of AtAMT1;3 facilitates the binding of NH4 + ions but loosely accommodates the binding of MeA+ to a more superficial location of the permeation pathway. Amongst point mutations tested based on this analysis, P181A resulted in both significantly increased current amplitudes and substrate binding affinity, whereas F178I led to opposite effects. Thus these 2 residues, which flank W179, a major structural component of the binding site, are also important determinants of AtAMT1;3 transport capacity by being involved in substrate binding. The Q365K mutation neighboring the histidine residue H378, which confines the substrate permeation tunnel, affected only the current amplitudes but not the binding affinities, providing evidence that Q365 mainly controls the substrate diffusion rate within the permeation pathway.
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Investigation of stable isotope composition under different land use types is helpful for understanding soil water movement and hydrological effects of land use change. We collected soil samples in profiles ï¼ 15 m deep under four land use types (i.e. farmland, grassland, Salix cheilophila and Populus sp.) in the loess deposits of northern Shaanxi. We measured hydrogen and oxygen stable isotope composition of soil water to explore the mechanism of soil water movement and the impacts of land use types. The isotope compositions of soil water under four land use types were significantly different. The δD values of soil water under farmland, grassland, S. cheilophila and Populus sp. were -81.1--60.1, -91.2--61.0, -87.4--63.6 and -73.5--62.2, while the δ18O values were -11.2--7.6, -12.6--8.2, -11.5--8.1 and -9.9--7.7, respectively. The soil water stable isotopes fluctuated across the profiles. The soil water isotope compositions in the layers of 0-3 m changed sharply, with the δD values being -80.2--61.8, -75.9--65.5, -76.0--63.6 and -73.5--62.2, respectively. In the layers of 3-12 m, the isotope profiles of farmland and grassland were parabolic, whereas those of S. cheilophila and Populus sp. were relatively stable. Soil water isotope compositions in the layers deeper than 12 m were generally stable with the δD values of -80.8--71.5, -83.0--67.5, -87.4--76.0 and -67.5--64.3, respectively. Across the four land use types, soil water stable isotope compositions were not significantly different either in the shallow layers or in the deep soil layers, but their differences in the layers of 3-12 m were significant. Soil moisture was mainly recharged from precipitation with piston flow as the main form of soil water movement. Soil water under four land use types might be recharged by wet events of different intensities. Soil water under farmland and grassland could be recharged by wet events of small intensity, but that under S. cheilophila and Populus sp. may be mainly recharged by the rainstorm in summer and autumn.
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Suelo , Agua , China , Hidrógeno , OxígenoRESUMEN
Two simple methods are proposed to respectively impart external force-free reversible shape memory effect to commercial polyolefins: ultrahigh molecular weight polyethylene (UHMWPE) and polypropylene (PP). The key issues lie in the utilization of the partially entangled molecular chains of UHMWPE and the medium crystalline phases of PP as the reversible internal stress providers. The acquired reversible shape memory effect further proves to be applicable for assisting repeatedly self-healing of wider cracks. Compared to the conventional approaches, which used to introduce cross-linkages into the target materials, the present ones only need physical treatment, so that the valuable thermoplasticity of polyolefins is retained. This work can be regarded as an example of the concept "physically converting instead of chemically modifying" for the preparation of functional polymeric materials based on market available plastics.
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To explore the effects of winter crops recycling into paddy field on soil carbon pool, we cultivated rape, Chinese milk vetch, ryegrass and potato in winter season after harvesting double-season rice, with fallow as control (CK). The contents of soil organic carbon (SOC) and labile organic carbon (LOC) in the harvest of early and late rice were measured, and the stable carbon, activity of carbon pool, carbon pool index, carbon pool activity index and carbon pool management index (CMI) were calculated. Results showed that winter cover crops returning increased SOC content by 1%-8% and 3%-18% after harvest of early rice and late rice, respectively in comparison with CK. Cultivating rape, Chinese milk vetch and ryegrass all increased the LOC content, with enhancement of 16.2%-84.2% and 24.4%-28.1% after harvest of early rice and late rice, respectively. The CMI of all winter crop treatments presented a growth ratio of 1.4%-41.8% compared to CK. In conclusion, the cultivation of winter crop and recycling to field promoted soil carbon fixation and soil quality, among which the ryegrass and Chinese milk vetch showed better comprehensive effect.
Asunto(s)
Agricultura/métodos , Oryza/crecimiento & desarrollo , Carbono/análisis , Estaciones del Año , Suelo/químicaRESUMEN
The present work is focused on developing external stress-free two-way triple shape memory polymers (SMPs). Accordingly, a series of innovative approaches are proposed for the material design and preparation. Polyurethane prepolymers carrying crystalline polytetrahydrofuran (PTMEG) and poly(ε-caprolactone) (PCL) as the switching phases are respectively synthesized in advance and then cross-linked to produce the target material. The stepwise method is believed to be conducive to manipulation of the relative contribution of PCL and PTMEG. Moreover, the chain extender, 2-amino-5-(2-hydroxyethyl)-6-methylpyrimidin-4-ol (UPy), is incorporated to establish hydrogen bonds among the macromolecules. By straightforward stretching treatment at different temperatures, the hydrogen bond networks are successfully converted into an internal stress provider, which overcomes the challenge of stress relaxation of the melted low melting temperature polymer (i.e., PTMEG) and increases the efficiency of stress transfer. Meanwhile, the contraction force of the switching phases is tuned to match the internal tensile stress. As a result, the internal stress provider can closely collaborate with melting/recrystallization of the crystalline domains, leading to the repeated multiple shape memory effects. The cross-linked polyurethane is thus able to reversibly morph among three shapes and displays its potentials as soft robot and actuator. The strategy reported here has the advantages of easily accessible raw materials, simple reaction, and facile programing/deprograming/reprograming, so that it possesses wide applicability.