A new virus, classifiable in the family Tombusviridae, found infecting Solanum tuberosum in the UK.

A novel virus was discovered in a freeze-dried collection held at SASA, UK, originating from potato (Solanum tuberosum) cv. Nadine. The complete sequence of the viral RNA was determined to be 3674 nucleotides in length encoding five predicted proteins. Based on the deduced genome organization and phylogenetic analysis, this virus represents a putative new member of the genus Alphanecrovirus, family Tombusviridae, most closely related to isolates of Olive mild mosaic virus. The virus was easily transmitted to indicator plants with symptoms that were slower to develop and less severe than those of related viruses. To distinguish this virus, the clearest symptom differences occurred with Nicotiana debneyi, Chenopodium amaranticolor and Ch. quinoa. The virus was detected with antisera to the related viruses tobacco necrosis virus A and tobacco necrosis virus D. The close association to the tobacco necrosis viruses would suggest this virus is not a new introduction to potato but in the past has been misidentified as one of these viruses. The virus isolate has been named potato necrosis virus.

Seasonal Abundance of Psyllid Species on Carrots and Potato Crops in Spain.

Psyllids (Hemiptera: Psylloidea) can transmit the phloem restricted bacterium 'Candidatus Liberibacter solanacearum' (Lso). In Europe, Lso causes severe losses to carrot and represents a threat to the potato industry. A rising concern is Lso transmission from carrot to potato and within potato, and this has driven the need for monitoring populations of psyllid species which could serve as vectors on both crops. This would provide a fundamental understanding of the epidemiology of Lso. Different sampling methods were used to survey populations of psyllid species in commercial carrot and potato fields in central and eastern mainland Spain from 2015 to 2017. Two psyllid species, Bactericera trigonica and Bactericera nigricornis were found on carrot and potato crops. In carrot fields the most abundant species was B. trigonica (occurring from crop emergence to harvest); whereas in potato crops the most abundant psyllid species was B. nigricornis. Depending on field location, the maximum psyllid populations occurred between June and October. Since B. nigricornis was found on both carrot and potato and is the only psyllid species able to feed and reproduce on both these crops in Europe, there is the potential risk of Lso transmission from carrot to potato.

Detection of Potato spindle tuber viroid and Other Related Viroids by a DIG Labelled RNA Probe.

Viroids can cause diseases of considerable economic importance; in Europe the main concern is with pospiviroids that may affect the tomato and potato industries. Methods for detection are required that are both sensitive and robust. The detection method described here is a probe hybridization method with a commercially available digoxigenin (DIG) labelled full-length Potato spindle tuber viroid (PSTVd) RNA probe. This method detects PSTVd and all other known pospiviroids.

Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes.

Phytoplasma infections are regularly reported worldwide, and concerns about their threats on agricultural production, especially in relation to global climate change, are increasing. Sensitive and reliable detection methods are important to ensure that propagation material is free of phytoplasma infection and for epidemiological studies that may provide information to limit the extent of phytoplasma diseases and to prevent large-scale crop losses. The detection method described here uses LNA chemistry in real-time PCR. It has been developed and validated for use on potatoes, and its sensitivity and specificity make it suitable for use in postentry potato quarantine and initiation of potato nuclear stocks to ensure that material is phytoplasma-free.