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The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus infects host cells by engaging its spike (S) protein with human ACE2 receptor. Recent studies suggest the involvement of integrins in SARS-CoV-2 infection through interaction with the S protein, but the underlying mechanism is not well understood. This study investigated the role of integrin α5ß1, which recognizes the Arg-Gly-Asp (RGD) motif in its physiological ligands, in S-mediated virus entry and cell-cell fusion. Our results showed that α5ß1 does not directly contribute to S-mediated cell entry, but it enhances S-mediated cell-cell fusion in collaboration with ACE2. This effect cannot be inhibited by the putative α5ß1 inhibitor ATN-161 or the high-affinity RGD-mimetic inhibitor MK-0429 but requires the participation of α5 cytoplasmic tail (CT). We detected a direct interaction between α5ß1 and the S protein, but this interaction does not rely on the RGD-containing receptor binding domain of the S1 subunit of the S protein. Instead, it involves the S2 subunit of the S protein and α5ß1 homo-oligomerization. Furthermore, we found that the S protein induces inflammatory responses in human endothelial cells, characterized by NF-κB activation, gasdermin D cleavage, and increased secretion of proinflammatory cytokines IL-6 and IL-1ß. These effects can be attenuated by the loss of α5 expression or inhibition of the α5 CT binding protein phosphodiesterase-4D (PDE4D), suggesting the involvement of α5 CT and PDE4D pathway. These findings provide molecular insights into the pathogenesis of SARS-CoV-2 mediated by a nonclassical RGD-independent ligand-binding and signaling function of integrin α5ß1 and suggest potential targets for antiviral treatment.
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COVID-19 , Integrina alfa5beta1 , Humanos , Integrina alfa5beta1/metabolismo , SARS-CoV-2/metabolismo , Células Endoteliales/metabolismo , Fusión Celular , Enzima Convertidora de Angiotensina 2 , Oligopéptidos/farmacología , Integrinas/química , Inflamación , Glicoproteína de la Espiga del Coronavirus/genéticaRESUMEN
SignificanceAdjusting the floating states when objects float on water shows great potential for assembly, mineral flotation, nanostructured construction, and floating robot design, but the real-time regulation of floating states is challenging. Inspired by the different floating states of a falling fruit, we propose a facile strategy to transform the object between different floating states based on a three-segment three-phase contact line evolution. In addition, the potential of floating state transformation in solar-powered water evaporation, interface catalysis, and drug delivery is demonstrated. These findings provide insights into floating regulation and show great potential for floating-related applications.
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Sistemas de Liberación de Medicamentos , Nanoestructuras , AguaRESUMEN
Ethylene glycol is an essential commodity chemical with high demand, which is conventionally produced via thermocatalytic oxidation of ethylene with huge fossil fuel consumption and CO2 emission. The one-step electrochemical approach offers a sustainable route but suffers from reliance on noble metal catalysts, low activity, and mediocre selectivity. Herein, we report a one-step electrochemical oxidation of ethylene to ethylene glycol over an earth-abundant metal-based molecular catalyst, a cobalt phthalocyanine supported on a carbon nanotube (CoPc/CNT). The catalyst delivers ethylene glycol with 100% selectivity and 1.78 min-1 turnover frequency at room temperature and ambient pressure, more competitive than those obtained over palladium catalysts. Experimental data demonstrate that the catalyst orchestrates multiple tasks in sequence, involving electrochemical water activation to generate high-valence Co-oxo species, ethylene epoxidation to afford an ethylene oxide intermediate via oxygen transfer, and eventually ring-opening of ethylene oxide to ethylene glycol facilitated by in situ formed Lewis acid site. This work offers a great opportunity for commodity chemicals synthesis based on a one-step, earth-abundant metal-catalyzed, and renewable electricity-driven route.
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Predator-prey arms races are ideal models for studying the natural selection and adaptive evolution that drive the formation of biological diversity. For venomous snakes, venom is a key bridge linking snakes with their prey, but whether and how venom evolves under the selection of diet remains unclear. Here, we focused on two closely related sea snakes, Hydrophis cyanocinctus and Hydrophis curtus, which show significant differences in prey preferences. Data-independent acquisition (DIA)-based proteomic analysis revealed different degrees of homogeneity in the venom composition of the two snakes, which was consistent with the differential phylogenetic diversity of their prey. By investigating the sequences and structures of three-finger toxins (3FTx), a predominant toxin family in elapid venom, we identified significant differences between the two sea snakes in the binding activity of 3FTx to receptors from different prey populations, which could explain the trophic specialization of H. cyanocinctus. Furthermore, we performed integrated multiomic profiling of the transcriptomes, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteomes of the venom glands; constructed venom-related mRNA-miRNA-lncRNA networks; and identified a series of noncoding RNAs involved in the regulation of toxin gene expression in the two species. These findings are highly informative for elucidating the molecular basis and regulatory mechanisms that account for discrepant venom evolution in response to divergent diets in closely related snakes, providing valuable evidence for the study of coselection and coevolution in predator-prey ecosystems.
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Hydrophiidae , Animales , Filogenia , Ecosistema , Proteómica , Multiómica , Venenos Elapídicos/química , Venenos Elapídicos/genéticaRESUMEN
Glaucoma is a complex neurodegenerative disorder characterized by the progressive loss of retinal ganglion cells (RGC) and optic nerve axons, leading to irreversible visual impairment. Despite its clinical significance, the underlying mechanisms of glaucoma pathogenesis remain poorly understood. In this study, we aimed to unravel the multifaceted nature of glaucoma by investigating the interaction between T cells and retinas. By utilizing clinical samples, murine glaucoma models, and T cell transfer models, we made several key findings. Firstly, we observed that CD4+ T cells from glaucoma patients displayed enhanced activation and a bias towards T helper (Th) 1 responses, which correlated with visual impairment. Secondly, we identified the infiltration of Th1 cells into the retina, where they targeted RGC and integrated into the pro-inflammatory glial network, contributing to progressive RGC loss. Thirdly, we discovered that circulating Th1 cells upregulated vascular cell adhesion protein 1 (VCAM-1) on retinal microvessels, facilitating their entry into the neural retina. Lastly, we found that Th1 cells underwent functional reprogramming before reaching the retina, acquiring a phenotype associated with lymphocyte migration and neurodegenerative diseases. Our study provides novel insights into the role of peripheral CD4+ T cells in glaucoma pathogenesis, shedding light on the mechanisms underlying their infiltration into the retina and offering potential avenues for innovative therapeutic interventions in this sight-threatening disease.
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Glaucoma , Células Ganglionares de la Retina , Humanos , Ratones , Animales , Células Ganglionares de la Retina/patología , Molécula 1 de Adhesión Celular Vascular/metabolismo , Células TH1/patología , Glaucoma/metabolismo , Retina/patología , Trastornos de la Visión/patología , Modelos Animales de EnfermedadRESUMEN
BACKGROUND: Syntaxin6 (STX6) is a SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptors) protein complex located in the trans-Golgi network and endosomes, which is closely associated with a variety of intracellular membrane transport events. STX6 has been shown to be overexpressed in a variety of human malignant tumors such as esophageal, colorectal, and renal cell carcinomas, and participates in tumorigenesis and development. METHODS: Based on clinical public database and clinical liver samples analysis, the expression of STX6 in hepatocellular carcinoma (HCC) tissues was investigated. The effects of STX6 on proliferation, migration and invasion of HCC cell in vitro and in vivo were evaluated through gain- and loss-of-function studies. We further performed RNA-seq analysis and protein interactome analysis, to further decifer the detailed mechanisms of STX6 in the regulation of the JAK-STAT pathway in HCC. RESULTS: STX6 expression was upregulated in HCC tissues and its expression was highly correlated with the high histological grade of the tumor. STX6 promoted HCC cell proliferation, migration and invasion both in vitro and in vivo. Mechanistically, STX6 mediated tumor progression depending on promoting the activation of JAK-STAT signaling pathway. Receptor for activated protein kinase C (RACK1) as an essential adaptor protein mediating STX6 regulation of JAK-STAT pathway. Specifically, STX6 interacted with RACK1 and then recruited signal transducer and activator of transcription 3 (STAT3) to form a protein-binding complex and activates STAT3 transcriptional activity. CONCLUSIONS: This study provided a novel concept that STX6 exerted oncogenic effects by activating the STAT3 signaling pathway, and STX6 might be a promising therapeutic target for HCC.
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INTRODUCTION: This study examines the associations of gum treatment with cognitive decline and dementia risk among older adults with periodontal symptoms in the US. METHODS: A cohort of 866 adults aged ≥50 with periodontal symptoms was recruited for the 2008 Health and Retirement Study "Dental Health Experimental Module" and followed until 2020. Cognitive function was assessed with the Telephone Interview for Cognitive Status (TICS). Dementia status was ascertained with the Langa-Weir algorithm based on TICS scores and proxy assessments. Linear mixed-effects model and multivariable Cox regression models were utilized to analyze the associations of gum treatment with cognitive decline and the risk of dementia, respectively. RESULTS: Of 866 participants (mean age 67.7, 61.4% women), 105 (12.1%) developed dementia with a median 9 (IQR, 6-10) years follow-up. The dementia incidence rates were lower in the group with gum treatment (7.4 vs. 12.9 per 1,000 person-years). Compared with participants who did not have gum treatment, those with gum treatment experienced a decline in TICS score that was on average 0.025 (95% CI, 0.005-0.044) points less per year and a 38% lower incidence of dementia (Hazard Ratio, 0.62; 95% CI, 0.41-0.93). These associations were consistent across participants with different severity of periodontal symptoms and sociodemographic characteristics (age, sex, race, ethnicity, and education) except for income levels. CONCLUSION: Prompt gum treatment for older adults with periodontal symptoms may be beneficial for their cognitive health.
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Interfacial solar steam generation for sustainable and eco-friendly desalination and wastewater treatment has attracted much attention. However, costly raw materials and complex preparation processes pose constant challenges to its wide promotion. Herein, a novel, cost-effective, and scalable strategy is presented for preparing solar interface evaporators using industrial waste as a raw material. Modified polyethylene foam evaporators (M-EPEs) are simply prepared by drilling and then hydrophilic modification of industrial waste (EPE-1). M-EPEs not only retain the strong mechanical properties and thermal insulating properties (0.047 W·m-1·K-1) of EPE-1 but also exhibit superhydrophilicity and strong light absorption (over 90%). M-EPEs achieve a high evaporation rate of 1.497 kg·m-2·h-1 and photothermal efficiency of up to 93.8% under 1 kW·m-2 solar illumination. Moreover, it has excellent stability and salt tolerance. Our work addresses the environmental issues of recycling polyethylene waste and provides a facile and efficient strategy for designing low-cost, large-scale solar interface evaporators for desalination.
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Aerogels have been widely studied in the field of thermal insulation. Herein, we reported a kind of conjugated micropolymer (CMP) aerogel synthesized by 1,3,5-triethynylbenzene and 2-amino-3,5-dibromopyridine. To enhance the flame-retardant property, we composited hydroxyapatite (HAP) nanowires with a CMP aerogel. Transmission electron microscopy (TEM) analysis revealed that HAP nanowires were encapsulated within nanosized CMP tubes. In addition, the thermal conductivity of HAP2-NCMP aerogel was 0.0251 W m-1 K-1, which possesses good thermal insulation property. In the micro-combustion calorimeter (MCC) test, compared with pure NCMP, the peak heat release rate (pHRR) of HAP2-NCMP decreased from 39.3 to 30.82 W g-1, approximately 21.6% lower. Furthermore, with the increased addition of hydroxyapatite in the HAP-NCMP composite, the pHRR of HAP3-NCMP decreased by about 37.4%. Besides, NCMP possesses good mechanical properties, with a compressive strength of 117.3 kPa at a strain level of 60%. These findings suggest promising application potential for HAP-NCMP in energy-saving and flame-retardant applications.
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Three psychrophilic bacteria, designated as strains SQ149T, SQ345T, and S1-1T, were isolated from deep-sea sediment from the South China Sea. All three strains were the most closely related to Thalassotalea atypica RZG4-3-1T based on the 16S rRNA gene sequence analysis (similarity ranged from 96.45 to 96.67â%). Phylogenetic analysis based on the 16S rRNA gene and core-genome sequences showed that three strains formed a cluster within the genus Thalassotalea. The average amino acid identity, average nucleotide identity, and digital DNA-DNA hybridization values among the three strains and closest Thalassotalea species were far below the cut-off value recommended for delineating species, indicating they each represented a novel species. All three strains were Gram-stain-negative, rod-shaped, and contained summed feature 3 (C16â:â1 ω7c and/or C16â:â1 ω6c) as the predominant fatty acid, Q-8 as the major respiratory quinone, and phosphatidylethanolamine and phosphatidylglycerol as predominant polar lipids. Based on the genomic, phylogenetic, and phenotypic characterizations, each strain is considered to represent a novel species within the genus Thalassotalea, for which the names Thalassotalea psychrophila sp. nov. (type strain SQ149T=MCCC 1K04231T=JCM 33807T), Thalassotalea nanhaiensis sp. nov. (type strain SQ345T=MCCC 1K04232T=JCM 33808T), and Thalassotalea fonticola sp. nov. (type strain S1-1T=MCCC 1K06879T=JCM 34824T) are proposed.
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Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano , Ácidos Grasos , Sedimentos Geológicos , Hibridación de Ácido Nucleico , Filogenia , ARN Ribosómico 16S , Agua de Mar , Análisis de Secuencia de ADN , Sedimentos Geológicos/microbiología , ARN Ribosómico 16S/genética , ADN Bacteriano/genética , Ácidos Grasos/química , China , Agua de Mar/microbiologíaRESUMEN
PURPOSE: To examine the relationship between hyperdense artery sign (HAS)/susceptibility vessel sign (SVS) and thrombus composition and evaluate the effect of HAS/SVS status on the association between first-line thrombectomy techniques and outcomes in patients with acute anterior circulation large vessel occlusion (LVO). MATERIALS AND METHODS: From January 2018 to June 2021, 103 consecutive patients with acute anterior circulation LVO (75 [63.1%] men; median age, 66 years) who underwent thrombectomy and for whom the removed clot was available for histological analyses were retrospectively reviewed. The presence of HAS and SVS was assessed on unenhanced computed tomography (CT) and susceptibility-weighted imaging, respectively. Association of first-line thrombectomy techniques (stent retriever [SR] combined with contact aspiration [CA] vs CA alone) with outcomes was assessed according to HAS/SVS status. RESULTS: Among the included patients, 55 (53.4%) were HAS/SVS-negative, and 69 (67.0%) underwent first-line SR + CA. Higher relative densities of fibrin/platelets (0.56 vs 0.51; P < .001) and lower relative densities of erythrocytes (0.32 vs 0.42; P < .001) were observed in HAS/SVS-negative patients compared with HAS/SVS-positive patients. First-line SR + CA was associated with reduced odds of distal embolization (adjusted odds ratio, 0.18; 95% CI, 0.04-0.83; P = .027) and a more favorable 90-day functional outcome (adjusted odds ratio, 5.29; 95% CI, 1.06-26.34; P = .042) in HAS/SVS-negative patients and a longer recanalization time (53 vs 25 minutes; P = .025) and higher risk of subarachnoid hemorrhage (24.2% vs 0%; P = .044) in HAS/SVS-positive patients. CONCLUSIONS: Absence of HAS/SVS may indicate a higher density of fibrin/platelets in the thrombus, and first-line SR + CA yielded superior functional outcomes than CA alone in patients with acute LVO without HAS/SVS.
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Procedimientos Endovasculares , Stents , Trombectomía , Humanos , Masculino , Femenino , Trombectomía/efectos adversos , Trombectomía/instrumentación , Estudios Retrospectivos , Anciano , Resultado del Tratamiento , Persona de Mediana Edad , Succión , Procedimientos Endovasculares/instrumentación , Procedimientos Endovasculares/efectos adversos , Valor Predictivo de las Pruebas , Factores de Riesgo , Anciano de 80 o más Años , Factores de Tiempo , Trombosis Intracraneal/diagnóstico por imagen , Trombosis Intracraneal/terapia , Trombosis Intracraneal/fisiopatologíaRESUMEN
The ß-catenin/B-cell lymphoma 9 (BCL9) protein-protein interaction (PPI) is a potential target for aberrantly active Wnt/ß-catenin signaling which actively participates in initiating and progressing of many cancers. Herein, we discovered novel 8-substituted quercetin derivatives with potential inhibitory activities targeting ß-catenin/BCL9 PPI. Among all the derivatives, compound B4 displayed the most promising PPI inhibitory activity with an IC50 value of 2.25 µM in a competitive fluorescence polarization assay and a KD value of 1.44 µM for the ß-catenin protein. Furthermore, B4 selectively inhibited the growth of colorectal cancer (CRC) cells, suppressed the transactivation of Wnt signaling, and downregulated the expression of oncogenic Wnt target gene. Especially, B4 showed potent anti-CRC activity in vivo with the tumor growth inhibition (TGI) of 75.99 % and regulated the tumor immune microenvironment.
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Neoplasias Colorrectales , Linfoma de Células B , Neoplasias , Quercetina , Humanos , beta Catenina/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular , Neoplasias Colorrectales/tratamiento farmacológico , Linfoma de Células B/tratamiento farmacológico , Proteínas de Neoplasias/metabolismo , Quercetina/farmacología , Microambiente Tumoral , Vía de Señalización WntRESUMEN
The ligands, derived from the combination of phenanthroline and various five-membered N-heterocyclic rings, were subject to a comprehensive investigation for their potential in the extraction and separation of actinides and lanthanides. This study employed DFT methods to thoroughly explore the properties of both phenanthroline (Ph) and the diverse five-membered N-heterocyclic rings (R1-R8). Additionally, tridentate ligands RlPh (l = 1-8) and tetradentate ligands RlPhRr (l, r = 1-8) were analyzed in detail, encompassing their electrostatic potential (ESP), protonation energy, coordination bonding with the metals Am(III) and Eu(III), and the thermodynamics of extraction separation for Am(III) and Eu(III). The findings highlight that the electrostatic potential (ESP) and binding capabilities of the five-membered N-heterocyclic ring units serve as effective predictors for the properties of intricate tridentate and tetradentate ligands, as well as their coordination bonding affinity with metals. The ligands' binding energy is closely associated with their ESP, and notably, the binding energy of tridentate and tetradentate ligands correlates well with the binding energies of their constituent structural units. The computational results reveal that the R2 unit, along with its corresponding tridentate ligand R2Ph and tetradentate ligands R2PhRr, exhibits the highest ESP, superior binding energies, and the strongest coordination bonding affinity with the metals. The theoretical calculations further identify several promising extractants for the effective separation of Am(III) and Eu(III). The tridentate ligands R1Ph, R7Ph, and R4Ph, and the tetradentate ligands R4PhR4, R6PhR6, R2PhR2, R1PhR5 and R3PhR6 were identified as having excellent separation performance for Am(III) and Eu(III). This study would provide insights for the design of extractants for the separation of Am(III) and Eu(III) by use of five-membered N-heterocyclic rings as structural units.
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The separation of trivalent actinide elements from lanthanide elements represents one of the most formidable challenges within the context of nuclear waste partitioning and transmutation (P&T) processes. Consequently, we embarked on a systematic investigation aimed at elucidating the bonding properties and thermodynamic behavior of a N-ethyl-N-tolyl-2-amide-1,10-phenanthroline (Et-Tol-PTA) ligand in conjunction with trivalent actinide and lanthanide elements. This investigation involved the utilization of various density functional theory (DFT) methods and a comparative analysis between small-core pseudopotential basis sets and all-electron basis sets. It was found that well-performing results were achieved using the PBE0 functional in both bond length and thermodynamic energy calculations, with minimal impact being exerted by the basis set on the results. Furthermore, an exploration was carried out into the bonding and thermodynamic properties of trivalent actinides and lanthanides with ligands derived from Et-Tol-PTA, encompassing non-rigid (La), partially rigid (Lb, Lc), and rigid (Ld) ligands. Thermodynamically, advantages in the separation of Am(III)/Eu(III) were exhibited by Lb and Lc ligands, while excellent performance in the separation of Am(III)/Cm(III) was demonstrated by the La ligand. Analyses conducted using quantum theory of atoms in molecules (QTAIM), reduced density gradient (RDG), and natural bond orbital (NBO) methodologies revealed the presence of partial covalent character in the bonds between oxygen (O) and metal (M), as well as between nitrogen (N) and metal (M), with a higher degree of covalent character being observed in O-Am and N-Am bonds compared to O-Cm/Eu and N-Cm/Eu interactions.
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PURPOSE: The optimal primary recanalization strategy for intracranial atherosclerosis-related emergent large vessel occlusion (ICAS-ELVO) remains controversial. We aimed to explore the safety and efficacy of balloon angioplasty as the first-choice recanalization strategy for ICAS-ELVO with small clot burden. METHODS: Consecutive ICAS-ELVO patients presenting with microcatheter "first-pass effect" during endovascular treatment (EVT) were retrospectively analyzed. Patients were divided into preferred balloon angioplasty (PBA) and preferred mechanical thrombectomy (PMT) groups based on the first-choice recanalization strategy. The reperfusion and clinical outcomes between the two groups were compared. RESULTS: Seventy-six patients with ICAS-ELVO involving the microcatheter "first-pass effect" during EVT were enrolled. Compared with patients in the PMT group, those in the PBA group were associated with (i) a higher rate of first-pass recanalization (54.0% vs. 28.9%, p = .010) and complete reperfusion (expanded thrombolysis in cerebral ischemia ≥ 2c; 76.0% vs. 53.8%, p = .049), (ii) shorter puncture-to-recanalization time (49.5 min vs. 89.0 min, p < .001), (iii) lower operation costs (¥48,499.5 vs. ¥ 99,086.0, p < .001), and (iv) better 90-day functional outcomes (modified Rankin scale:0-1; 44.0% vs. 19.2%, p = .032). Logistic regression analysis revealed that balloon angioplasty as the first-choice recanalization strategy was an independent predictor of 90-day excellent functional outcomes for ICAS-ELVO patients with microcatheter "first-pass effect" (adjusted odds ratio = 6.01, 95% confidence interval: 1.15-31.51, p = .034). CONCLUSION: Direct balloon angioplasty potentially improves 90-day functional outcomes for ICAS-ELVO patients with small clot burden, and may be a more appropriate first-choice recanalization strategy than mechanical thrombectomy for these patients.
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Angioplastia de Balón , Arteriosclerosis Intracraneal , Accidente Cerebrovascular , Humanos , Accidente Cerebrovascular/cirugía , Estudios Retrospectivos , Trombectomía , Arteriosclerosis Intracraneal/diagnóstico por imagen , Arteriosclerosis Intracraneal/terapia , Arteriosclerosis Intracraneal/complicaciones , Resultado del TratamientoRESUMEN
According to the green development requirements of carbon neutrality and carbon peaking, the effective separation of lanthanides and actinides is one of the key factors for nuclear energy to become a sustainable energy source. In recent years, o-phenanthroline-based ligands have been proven to be effective in the separation of lanthanides and actinides. In this work, based on 5,9b-dihydro-4aH-cyclopenta[1,2-b:5,4-b']dipyridines, we designed six N-heterocyclic ring ligands and theoretically studied their extraction capacity and separation selectivity for the Am(III) and Eu(III) ions. Various theoretical methods were used to analyze the properties of the ligands and study the bonding nature of the ligands with the metal ions. Thermodynamic parameters were calculated to measure the extraction ability of the ligands to the metal ions and to explore the separation capacity of the ligand for the Am(III) and Eu(III) ions. The calculated results show that the five- and six-membered N-heterocyclic ring side chains of the ligands and the distribution of the N atoms on the side chain rings have obvious effects on the bonding of the ligands to metal ions and on the extraction and separation properties of the ligands for the metal ions. It was found that the extractants with six-membered ring side chains possess an extraction ability slightly better than that of the ligands with five-membered ring side chains and that the ligands with a pair of adjacent N atoms on the side chains have a stronger separation selectivity for the Am(III)/Eu(III) ions. The theoretical research in this work will help to understand the details of binding and extraction properties of similar ligands and provide insights for the future design of five- and six-membered heterocyclic ligands.
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AIM: To assess the relationship between dietary antioxidant intake and periodontal health in US adults and the potential role of mitochondrial function. MATERIALS AND METHODS: We performed a cross-sectional study using data from the National Health and Nutrition Examination Survey (NHANES) 2011-2014. Dietary antioxidant intake was evaluated using three diet-related indices: dietary oxidative balance score (DOBS), dietary total antioxidant capacity (DTAC) of antioxidant vitamins and composite dietary antioxidant index (CDAI). Periodontal parameters included attachment loss (AL) and probing pocket depth (PPD). Mitochondrial dysfunction was assessed using the methylmalonic acid (MMA) level. Weighted multivariable linear regression analyses were employed to investigate the association between dietary antioxidant intake and periodontal status. Additionally, exploratory mediation analyses were conducted to determine the mediating effect of MMA on the association. RESULTS: Totally, 5520 participants were included in our study. Participants with higher DOBS and DTAC scores had lower mean AL/PPD and MMA values. CDAI was negatively associated with mean AL and PPD. Furthermore, MMA mediated 9.4% and 4.9% of the associations between DOBS and mean AL and mean PPD, respectively. MMA also accounted for 7.2% and 3.3% of the association between DTAC and mean AL and PPD, respectively. CONCLUSIONS: The findings support that dietary antioxidant intake helps in improving periodontal health, possibly and partially by enhancing mitochondrial function.
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Antioxidantes , Dieta , Mitocondrias , Encuestas Nutricionales , Humanos , Antioxidantes/administración & dosificación , Masculino , Femenino , Estudios Transversales , Adulto , Persona de Mediana Edad , Estados Unidos , Análisis de MediaciónRESUMEN
PURPOSE: Mucosal inflammation is a key feature of ulcerative colitis (UC), a chronic relapsing and remitting form of inflammatory bowel disease. Omentin-1, a newly discovered adipokine, is reported to have anti-inflammatory effects and has been found to be decreased in patients with inflammatory bowel disease. The aim of our study was to investigate the association between serum omentin-1 levels and mucosal disease activity in patients with UC. STUDY DESIGN: A total of 126 patients with UC and 77 healthy volunteers were enrolled in the study. Serum omentin-1 expression levels were measured using enzyme-linked immunosorbent assay to evaluate its potential for monitoring disease activity, including clinical and endoscopic activity. RESULTS: Serum omentin-1 levels were significantly lower in patients with UC compared to healthy controls (HC) (UC, 61.7 interquartile range: 51.5-72.6 versus healthy controls, 103.5 interquartile range: 48.3-156.2 ng/ml; P < .001). Furthermore, serum omentin-1 levels were associated with both clinical and endoscopic activity in patients with UC. Notably, omentin-1 levels were significantly lower in patients who achieved mucosal healing. Receiver operating characteristic curves indicated that serum omentin-1 levels could potentially serve as an activity index for evaluating UC. CONCLUSIONS: These findings provide further insight into the association between omentin-1 and UC, suggesting that omentin-1 may be a useful biomarker for monitoring mucosal disease activity in patients with UC.
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Biomarcadores , Colitis Ulcerosa , Citocinas , Proteínas Ligadas a GPI , Lectinas , Humanos , Colitis Ulcerosa/sangre , Proteínas Ligadas a GPI/sangre , Lectinas/sangre , Citocinas/sangre , Masculino , Femenino , Adulto , Biomarcadores/sangre , Persona de Mediana Edad , Estudios de Casos y Controles , Mucosa Intestinal/metabolismo , Ensayo de Inmunoadsorción EnzimáticaRESUMEN
Adipocyte play an important role in human health and meat quality by influencing the tenderness, flavor, and juiciness of mutton It has been shown that neuron-derived neurotrophic factor (NENF) is closely related to energy metabolism and adipocyte differentiation in bovine. However, the role of NENF in the goats remains unclear. The aim of this study was to detect the expression of NENF in goat subcutaneous and intramuscular adipocytes, temporal expression profiles of the NENF, and overexpressed NENF on the differentiation of different adipocytes. In this study, PCR amplification successfully cloned the goat NENF gene with a fragment length of 521 bp. In addition, the time point of highest expression of NENF differed between these two adipocytes differentiation processes. Overexpression of NENF in intramuscular and subcutaneous adipocytes promoted the expression levels of differentiation markers CEBPß and SREBP, which in turn promoted the differentiation of intramuscular and subcutaneous adipocytes. This study will provide basic data for further study of the role of goats in goat adipocyte differentiation and for the final elucidation of its molecular mechanisms in regulating goat adipocyte deposition.