Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.

Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.

Synthesis, photophysics, and reverse saturable absorption of bipyridyl platinum(II) bis(acetylide) complexes bearing aromatic electron-withdrawing substituents on the acetylide ligands.

Three platinum(II) bipyridyl bis((7-R-fluoren-2-yl)acetylide) complexes (R = benzoyl (Pt-1), 2-(N-phenylbenzimidazoly) (Pt-2), or 2-(3-phenylquinoxalinyl) (Pt-3)) are synthesized and characterized. Their photophysical properties and reverse saturable absorption are systematically investigated via UV-vis absorption, emission, transient absorption, and nonlinear transmission spectroscopy/technique. All three complexes possess ligand-centered (1)π,π* transitions below 400 nm, and a broad, featureless (1)MLCT/(1)LLCT absorption band in the region of 400-550 nm in CH2Cl2 solutions. They are emissive in a variety of fluid solutions at room temperature and at 77 K glassy matrix. On the basis of the emission lifetime, solvatochromic effect, and thermally induced Stokes shift, the emitting states are tentatively ascribed to (3)π,π*/(3)MLCT/(3)LLCT states for Pt-1, and predominantly (3)MLCT/(3)LLCT states for Pt-2 and Pt-3 in polar solvents like CH3CN. Pt-1-Pt-3 also exhibit broad triplet excited-state absorption, i.e., 425-800 nm for Pt-1 and Pt-3, and 425-725 nm for Pt-2, from the same excited states that emit. Strong reverse saturable absorption (RSA) occurs at 532 nm for ns laser pulses from all of the complexes due to the stronger triplet excited-state absorption at this wavelength, suggesting that the π-conjugated aromatic electron-withdrawing substituents at the fluorenylacetylide ligands enhance the RSA of the Pt(II) diimine bis(acetylide) complexes.

Orange-emissive carbon quantum dots for ligand-directed Golgi apparatus-targeting and in vivo imaging.

The Golgi apparatus is one of the most important organelles in cells. Targeting and monitoring the morphology and structure of the Golgi apparatus are crucial and challenging. Aimed at the cysteine (Cys) receptor on the surface of the Golgi apparatus, ligand-directed carbon quantum dots (CQDs) were synthesized for Golgi apparatus-targeting imaging. In order to reduce the interference of tissue self-fluorescence and enhance the tissue penetration depth, orange-emissive levorotatory CQDs (L-CQDs) with Golgi apparatus-targeting ability were synthesized using the strategy of inheriting Cys residues and the inherent conjugated electronic structure of neutral red. They exhibit excitation-dependent, fluorescence stability, rich surface Cys residues, excellent biocompatibility, and low toxicity. As a Golgi apparatus-targeting agent, L-CQDs can quickly enter cells for Golgi apparatus-targeting imaging, and can also penetrate through biological tissue for imaging in vivo. The surface Cys residues of CQDs actively target the Cys receptors on the surface of the Golgi apparatus to achieve Golgi apparatus-targeting imaging.

[Spectral analysis and band gap of RbVO3].

In the present paper, RbVO3 was prepared by wet chemistry synthesis from hybrid precursor. The structure of RbVO3 was characterized by X-ray diffraction (XRD) pattern and FTIR spectra. Photoluminescent (PL) properties were investigated by UV-Vis absorption spectrum and PL spectrum. In addition, the band gap of RbVO3 was calculated by using the CASTEP code with density-functional theory (DFT) method. The results indicated that RbVO3 could emit intense green-white fluorescence with peak wavelength at 525 nm and the color coordinates was (0.318 0, 0.430 9) under UV excitation at 357 nm. It was demonstrated that the theoretical result of band gap, which is 2.67 eV, agreed well with the experiment.

The reaction of acyl cyanides with "Huisgen zwitterion": an interesting rearrangement involving ester group migration between oxygen and nitrogen atoms.

A novel rearrangement involving ester group migration was found in the reaction of acyl cyanides and Huisgen zwitterions, affording hydrazone derivatives at higher temperature (90 degrees C) and azine derivatives at lower temperature (20 degrees C), respectively. Interestingly, the reaction temperature is identified as a critical factor to control the final products. Presumably, the rearrangement involving ester group migration between oxygen and nitrogen atoms leads to the formation of different products.

[Characterization and photoluminescence properties of a blue-light-emitting material].

Tris (2-methyl-8-hydroxyquinoline) aluminum (AlMeq3) was synthesized and purified by vacuum sublimation. The structure of the complex was characterized by 1H NMR, FTIR spectra and elemental analysis techniques. AlMeq3 consists of three 2-methyl-8-hydroxyquinoline ligands and one aluminum atom. Its thermal stability was studied by TG and DSC analysis and the result shows that AlMeq3 is a thermally stable material, with decomposition and crystalline transition temperature being 357 and 158 degrees C, respectively. Energy band structure was investigated by UV-Vis absorption spectra and fluorescence emission spectra. Experimental results show that its UV absorption bands were at about 246 and 390 nm, and the absorption band at about 246 nm can be assigned to pi--pi* of phenyl ring. AlMeq2 displays 8-hydroxyquinoline-oriented photophysical properties. The optical gap of AlMeq3: was about 2.85 eV, as determined by intrinsic absorption band edge of the complex in ethanol solution. Under UV excitation at 365 nm, the complex in ethanol solution emitted intensive blue fluorescence with the maximum emission peak at 479 nm, while the effective energy-transfer from the ligand to the central AlP+ ion occurred in the complex. AlMeq3 with bright blue photoluminescence can be applied as luminescent material in OLEDs.

Asymmetric Morita-Baylis-Hillman reaction of arylaldehydes with 2-cyclohexen-1-one catalyzed by chiral bis(thio)urea and DABCO.

Novel bis(thio)urea organocatalysts were synthesized from axially chiral (R)-(-)-5,5',6,6',7,7',8,8'-octahydro-1,1'-binaphthyl-2,2'-diamine (H8-BINAM), and their catalytic abilities have been examined in the Morita-Baylis-Hillman reaction of 2-cyclohexen-1-one or 2-cyclopenten-1-one with a wide range of aromatic aldehydes in combination with DABCO. The best result was achieved in the reaction of 3-fluorobenzaldehyde with 2-cyclohexen-1-one to give the desired Morita-Baylis-Hillman product in 79% yield and 88% ee.

Asymmetric catalytic aza-Morita-Baylis-Hillman reaction (aza-MBH): an interesting functional group-caused reversal of asymmetric induction.

A highly efficient aza-Morita-Baylis-Hillman reaction (aza-MBH reaction) of N-tosyl salicylaldehyde imines with alpha,beta-unsaturated ketones has been achieved by using beta-isocupreidine (beta-) as the catalyst (10 mol%) to give the corresponding adducts in good to high yields (90%-quant.) and excellent ee's (up to 99% ee), with adducts showing the opposite absolute configuration to that of those obtained in the similar aza-MBH reaction of N-tosyl aldimines with alpha,beta-unsaturated ketones.

Gold(I)-catalyzed cascade cyclization reaction: highly regio- and diastereoselective intermolecular addition of water and alcohols to epoxy alkynes.

We have developed a novel access to ketal skeletons through a highly regio- and diastereoselective intermolecular addition of water and alcohols to alkynyl epoxides catalyzed by gold(I). This procedure involves a domino three-membered ring-opening, 6-exo-cycloisomerization, and subsequent intra- or intermolecular nucleophilic addition to a double-bond sequence.

[Luminescence properties of white LED blue light conversion materials].

Using rare earth oxides as raw materials, yttrium aluminum garnets activated by cerium and gadolinium ions were prepared by high energy ball milling and solid-state reaction at 1300 degrees C. The crystal structures of the products were studied by XRD, and the luminescence characteristics of Ce ion and the influence of Gd ion were studied by emission and excitation spectra. The results indicated that the products were yttrium aluminum garnet crystals of cubic system, and they were excited by blue light chips effectively. The emission peak of phosphors could change among 530-560 nm by adjusting the mole density of doping ions. The red shift of emission spectra could be interpreted with the configuration coordination figure. White LEDs were fabricated by phosphor conversion. When electric current is 20 mA, and the working voltage is 3.5 V, the white light LED chromaticity coordinates are x=0.310 and y=0.323, the lumen efficiency is 26.131 m x W(-1), the color rendering index is 81.8, and the color temperature is 6605 K.

[Spectral analysis and photoluminescence properties of 1,5-naphthalene diamine derivative].

In the present paper, 1,5-bis[N-(1-naphthyl)-N-phenyl] naphthalene diamine (NPN) with high purity was synthesized by liquid-phase Ullmann reaction, and high quality film of NPN was formed on cleaned glass substrates by vacuum evaporation. Photoluminescence properties were studied by solvent effects on UV-Vis absorption spectra and fluorescence spectra. In addition the structure of energy band of NPN was studied by cyclic voltammetry, UV-Vis absorption spectra, and fluorescence spectra. The results indicate that the fluorescence emitting is attributed to the charge transfer transition from nitrogen to aromatic ring. The molecules of NPN in its film form "J-aggregate". The HOMO energy and optical gap of NPN are -5.74 and 2.79 eV respectively. NPN film could emit intense blue fluorescence with a peak wavelength at 448.6 nm and a bandwidth of 72.6 nm under UV-Vis excitation at 365 nm.

[Spectroscopic properties of salicylaldehyde anil zinc and its thin film].

A new light emitting material, salicylaldehyde anil zinc (SAZ), was synthesized. It can form high quality nano-scale amorphous thin films on clean glass substrates by vacuum evaporation. Its structure, crystallization, thermal stability, and optical property were investigated by IR spectra, DTA-TG analysis, XRD spectra, UV-Vis spectra, and fluorescence spectra. Its energy band structure was confirmed by cyclic voltammogram and optical absorption band edge. Results show that the SAZ film is a thermally stable material, and can emit intense green fluorescence with a peak wavelength at 508 nm and a full width at half-maximum of 90.2 nm under UV irradiation. Its HOMO energy level is about -5.659 eV, LUMO energy level is about -3.054 eV, optical gap band is about 2.604 eV. The fluorescence decay of stored films under ambient atmosphere is more rapid than that of 8-hydroxyquinoline aluminum films. However, the fluorescence decay of the films under UV irradiation is slower than that of 8-hydroxyquinoline aluminum films.

[Effects of Chronological Moxibustion on Circadian Rhythm Activities of Hypothalamus-Pituitary-Axis in Rheumatoid Arthritis Rats].

OBJECTIVE: To observe the effect of chronological moxibustion on plasma corticosterone (CORT), corticotrophin releasing factor (CRF) and adrenocorticotropic hormone (ACTH) levels in rheumatoid arthritis (RA) rats, so as to explore its mechanisms underlying improvement of RA. METHODS: A total of 144 SD rats were randomly divided into normal control, model, moxibustion, sham-adrenalectomy (ADX, sham-ADX), ADX and ADX+moxibustion groups which were further separately divided into 4 subgroups (4 time-points: 12 pm, 6 am, 12 am, 6 pm, n = 6/subgroup). The RA model was established by subcutaneous injection of Freund's complete adjuvant (FCA, 0. 1 mL) into the right footpad (for rats of the latter 5 groups). In rats of the ADX and ADX+ moxibustion groups, bilateral adrenal glands were removed under anesthesia. Moxibustion was applied to unilateral "Shenshu" (BL 23) and "Zusanli" (ST 36) for 10 min, once daily for 6 days in a week, and continuously for 3 weeks. The paw swelling volume was measured and plasma CORT, ACTH and CRF contents were assayed by ELISA and the circadian rhythm was analyzed by cosine curve fitting (cosinor) method. RESULTS: The paw swelling volume was significantly increased in the model group than in the normal control group (P<0.05), and decreased remarkably in the moxibustion group (P<0.05). The paw volume was markedly increased in the ADX +moxibustion group in comparison with the moxibustion group (P<0.05). In intact rats, plasma CRF and ACTH contents were significantly increased (P<0.05), and plasma CORT was obviously decreased in the model group (P<0.05). After moxibustion, the increased plasma CRF and ACTH contents and the decreased plasma CORT level Nere considerably reversed (P<0.05). In ADX rats, plasma ORE, ACTH and CORT contents of ADX and ADX + moxibustion groups had no significant.changes compared with the sham-ADX group (P>0.05). Results of the cosine curve fitting analysis showed that the peak phases of plasma CORT contents were -355.78 degrees at about 23:43 in the normal control group, -309.05 degrees at about 20:36 in the model group, -326.5 degrees at about 21:46 in the moxibustion group, -291.65 degrees at about 19:27 in the ADX group and -300.87 degrees at about 20:31 in the ADX + moxibustion group. The peak phases of plasma ACTH contents were -324.08 degrees at about 21:37 in the normal control group, -295.39 degrees at about 19:41 in the model group, -310.81 degrees at about 20:43 in the moxibustion group, -146.51 degrees at about 9:46 in the ADX group and -267.64 degrees at about 17:50 in the ADX+ moxibustion group. The peak phases of plasma CRF contents were -257.47 degrees at about 17:10 in the normal control group, -184.74 degrees at about 12:19 in the model group, -263.00 degrees at about 17:32 in the moxibustion group, -202.46 degrees at about 13:30 in the ADX group and -232.84 degrees at about 15:31 in the ADX+ moxibustion group. It suggests that in the intact RA rats, moxibustion intervention may inhibit modeling-induced decrease of the circadian rhythm of plasma CORT, and hyperactivity of ACTH and CRF circadian rhythm. CONCLUSION: VAoxibustion intervention can relieve paw swelling in RA rats and modulate the circadian rhythm activities of plasma CORT, ACTH and ORE levels (HPA axis activities) in intact animals.

[Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats].

OBJECTIVE: To observe the effect of electroacupuncture (EA) intervention on the levels of CD 4+ and CD 8+ lymphocytes in the plasma and thymus in anxiety disorder rats, so as to explore its mechanism underlying favorable regulation of immune function. METHODS: Thirty-four SD rats were randomly divided into control (n = 10) , model (n = 12) and EA (n = 12) groups. The anxiety model was established by using chronic unpredictable emotional stress stimulation (fasting, water-deprivation, shaking, tail-clamping, forced warm- and cool-water swimming, electrical shock stimulation, etc.) for 15 days. EA (15 Hz/ 25 Hz) was applied to "Neiguan" (PC 6) and "Shenmen" (HT 7) for 15 min, once every other day for 15 days. The anxiety-like behavior was measured by elevated plus maze test. Anxiety reduction in the plus-maze is indicated by an increase in the proportion of time spent in the open arms (time in open arms/total time in open or closed arms) , and an increase in the proportion of entries into the open arms (entries into open arms/total entries into open or closed arms). While the contents and expression of CD 4+ and CD 8+ in the plasma and thymus tissues were measured by ELISA and immunohistochemistry, respectively. RESULTS: The values of proportions of open-arm entries (OE%) and the content of plasma CD 4+ lymphocytes were obviously lower in the model group than in the control group (P < 0.05), and were significantly higher in the EA group than in the model group (P < 0.05). Whereas, the content of plasma CD 8+ lymphocytes was obviously higher in the model group than in the control group (P < 0.05) and markedly down-regulated in the EA group after the treatment (P < 0.05). The ratio of CD 4+/CD 8+ was decreased in the model group in comparison with the control group (P = 0.054), and was significantly up-regulated in the EA group after EA stimulation (P < 0.01). Compared with the control group, the expression levels (optical density, OD values) of CD 4+ and CD 8+ lymphocytes in the thymus tissue were obviously decreased in the model group (P < 0.01). After EA, the expression levels of thymus CD 4+ and CD 8+ were remarkably up-regulated in the EA group (P < 0.05). No significant changes were found in proportion of open-arm time (OT%, P > 0.05). CONCLUSION: EA can effectively relieve anxiety disorder in anxiety rats, which may be related to its effects in up-regulating plasma CD 4+ , thymus CD 4+ and CD 8' levels as well as CD 4+/CD 8+ ratio, and down-regulating plasma CD 8+ content.

[Preparation and characterization of 2(8-hydroxyquinoline)-2( phenol) zirconium thin film].

In this paper, the new emitting material zirconium complex with 8-hydroxyquinoline and phenol (Zr(OPh)2 q2), which can form high quality nano-scale amorphous thin films at glass substrates cleaned by vacuum evaporation, was synthesized. Its crystallization, thermal stability, the structure of energy band and PL mechanism were investigated respectively by FTIR spectra, DTA-TG analysis, SEM, XRD spectra, UV-Vis absorption spectra and fluorescence spectra. Results show that the Zr(OPh)2 q2 is a thermally stable material, whose melting point is 381.2 degrees C, decomposition temperature is 467.1 degrees C, whose film can emits intense yellow-green fluorescence with peak wavelength at 525 nm and a full width at half-maximum of 107.6 nm under UV excitation (390 nm), powder has intense yellow fluorescence, therefore it may be a excellent electroluminescent material.

Auxin transport and activity regulate stomatal patterning and development.

Stomata are two-celled valves that control epidermal pores whose spacing optimizes shoot-atmosphere gas exchange. They develop from protodermal cells after unequal divisions followed by an equal division and differentiation. The concentration of the hormone auxin, a master plant developmental regulator, is tightly controlled in time and space, but its role, if any, in stomatal formation is obscure. Here dynamic changes of auxin activity during stomatal development are monitored using auxin input (DII-VENUS) and output (DR5:VENUS) markers by time-lapse imaging. A decrease in auxin levels in the smaller daughter cell after unequal division presages the acquisition of a guard mother cell fate whose equal division produces the two guard cells. Thus, stomatal patterning requires auxin pathway control of stem cell compartment size, as well as auxin depletion that triggers a developmental switch from unequal to equal division.

[Effect of different types of moxibustion intervention on expression of inflammatory cytokines IL-1 and TNF-alpha in rabbits with rheumatoid arthritis].

OBJECTIVE: To observe the effect of different moxibustion intervention on expression of interleukin-1 (IL-l) and tumor necrosis factor-alpha (TNF-alpha) in the synovial fluid of hind-knee joint in rheumatoid arthritis (RA) rabbits. METHODS: Forty Japanese big-ear white rabbits (half male and half female) were randomized into normal control, RA model, direct-moxibustion, ginger-partitioned moxibustion and warm moxibustion groups (n= 8). RA model was established by injection of Freund's Complete Adjuvant (0. 5 mL/kg) into the articular cavities of the rabbits' bilateral hind-limbs. Moxibustion intervention was applied to unilateral "Shenshu" (BL 23) and "Zusanli"(ST 36) regions alternatively for 20 min from the 7th day on after modeling, once daily for 3 weeks except Sundays. The circumference of the hindlimb-knee joint was measured using a tape measure and the contents of IL-1 and TNF-alpha in the synovial fluid of articular cavities were detected by ELISA. RESULTS: In comparison with the normal control group, the circumference values of the bilateral hind-knee joints, and the contents of IL-1 and TNF-alpha in the synovial fluid of articular cavities in the model group were significantly increased (P<0. 01). After the moxibustion treatment, compared with the model group, the circumference values of the bilateral hind-knee joints, and IL-1 and TNF-alpha contents of the synovial fluid in the warm moxibustion, direct moxibustion and ginger-partitioned moxibustion groups were remarkably reduced (P<0.01, P<0.05). The effects of the ginger-partitioned group were significantly superior to those of both warm moxibustion and direct moxibustion groups in decreasing the swelled hind-knee joint circumference on day 21 after the treatment and down-regulating synovial fluid inflammatory cytokines IL-1 and TNF-alpha levels (P<0. 05). CONCLUSION: Warm, direct and ginger-separated moxibustion interventions all can reduce inflammatory reactions of the knee-joint and suppress inflammatory cytokine IL-1 and TNF-alpha levels of the synovial fluid in RA rabbits, which may contribute to its effect in improving RA in clinic. The therapeutic effect of ginger-partitioned muxibustion intervention is apparently better.

[Effects of moxibustion intervention on inflammatory reactions and expression of suppressor of cytokine signaling proteins of synovium cells in rheumatoid arthritis rabbits].

OBJECTIVE: To observe the effect of moxibustion intervention on inflammatory reactions and expression of suppressor of cyfokine signaling 1 (SOCS 1) and SOCS 2 [Which are involved in inhibition of the Janus Kinase-signal transducer and activator of transcrip-tion (JAK/STAT signaling pathway and in sffenuation of cytokine signaling)] in synovium cells of the hind-knee joint in rheumatoid arthritis (RA) rabbits, so as to study its mechanism underlying improvement of RA. METHODS: Forty-two Japanese big-ear white rabbits were randomized into control, model and moxibustion groups respectively, with 14 cases in each group. RA model was established by injection of Freund's Complete Adjuvant (0. 5 mL/kg) into the rabbits' bilateral hind-knee joint cavities. Moxibustion was applied to bilateral "Shenshu" (BL 23) areas, 5 cones every time, once daily for 3 weeks except the Sundays. The perimeters of rabbits' hind legs were measured before and after modeling and after the therapy. The synovial tissue of joint was sampled for analyzing the expression levels of SOCS 1 and SOCS 3 by immunohistochemistry. RESULTS: Before the therapy, the perimeters of bilateral knee joints of the control, model and moxibustion groups were of no statistical significance (P>0. 05). In comparison with the control group, the perimeters of bilateral knee joints were significantly increased on day 1, 7, 14 and 21 in the model group (P<0. 01). Compared with the model group, the perimeters of bilateral knee joints in the moxibustion group were significantly decreased (P<0. 05), suggesting an improvement of the inflammatory reaction after moxibustion intervention. Correspondingly, synovial SOCS 1 and SOCS 3 expression levels were remarkabely higher in the model group than in the control group (P<0. 01), and obviously decreased in the moxibustion group compared with the model group (P<0. 01). CONCLUSIONS: Moxibustion intervention has an anti-inflammatory and detumescent effects in RA rabbits, which may be closely associated with its effects in down-regulating expression of SOCS 1 and SOCS 3 proteins by suppressing negative feedback regulatory JAK/STAT pathway in synovial cells. [KEY WORDS] Moxibustion; Rheumatoid arthritis; Inflammatory reactions; Synovial cells; Suppressor of cytokine signaling proteins; Negative-feedback regulatory factors

[Impacts of acupuncture at Hegu (LI 4) on facial EMG in rhesus monkeys under the physiological state].

OBJECTIVE: To observe the specificity relationship between acupuncture at "Hegu" (LI 4) and the facial muscular movement in rhesus monkeys under the physiological state by using neuromuscular electrical measurement technique. METHODS: Eighteen rhesus monkeys were randomized into a Hegu group, a Houxi group and a Waiguan group, 6 monkeys in each one. Under the physiological state, EMG was detected on the frontal muscle, zygomatic muscle and orbicular muscle before and after acupuncture at different acupoints. The impacts of acupuncture on the facial EMG were studied and compared among different acupoints. RESULTS: With acupuncture at "Hegu" (LI 4), the latency was reduced (P < 0.01) and the peak value and area were increased (P < 0.05, P < 0.01) in the frontal EMG; the area and the peak value were increased (P < 0.01, P < 0.05) and latency was reduced (P < 0.05) in the zygomatic EMG; the frequency was increased (P < 0.01) and the latency was reduced (P < 0.05) in the orbicular EMG. Before and after acupuncture at "Hegu" (LI 4), the change rates of EMG frequency, peak value, area and latency on the frontal, zygomatic and orbicular muscles were higher than those at "Houxi" (SI 3) and "Waiguan" (TE 5) (P < 0.05, P < 0.01) separately. CONCLUSION: The relative specificity presents between Hegu (LI 4) and facial muscular movement.