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1.
Biofizika ; 55(2): 269-70, 2010.
Artículo en Ruso | MEDLINE | ID: mdl-20429280

RESUMEN

Changes in the surface potential of liposomes obtained from dipalmitoyl phospatidylcholine during their interaction with the new antiviral preparation boraadmantane have been studied. It has been concluded that the saturation of the lipid bilayer of liposomes by boraadmantane occurs at concentrations of the preparation above 10 microg/ml.


Asunto(s)
1,2-Dipalmitoilfosfatidilcolina/química , Adamantano/química , Antivirales/química , Membrana Dobles de Lípidos/química , Liposomas/química , Adamantano/análogos & derivados
2.
Artículo en Ruso | MEDLINE | ID: mdl-20095433

RESUMEN

AIM: To develop new method of determination of size and concentration of lyposomes based on measurement of opacity in dispersed media. MATERIALS AND METHODS: Dispersions of lyposomes from dipalmitoylposphatidylcholine were the object of the study. Opacity spectrum of lyposome suspension was measured with Zenyth 200st spectrophotometer. Mean values of diameter of lyposomes determined by opacity spectrum were compared with the same values measured by electron microscopy (JEM 100-CX, JEOL, Japan) with magnification 58,000 - 100,000. Refraction index was measured with refractometer RPL-3 (Russia). RESULTS: Sizes of lyposomes measured by the new method and by electron microscopy did not differ significantly. Determination of sizes and concentration of lyposomes by the new method did not depend from effect of secondary multiple scattering of light. CONCLUSION: Obtained data allowed to conclude that developed method could be used in practice. Advantages of the new method are usage of common spectrophotometers and photoelectrocolorimeters for deriving of liposomes suspension opacity curve as well as its high validity, which are confirmed by data obtained with electron microscopy.


Asunto(s)
Calorimetría/métodos , Liposomas/química , Tamaño de la Partícula , Espectrofotometría/métodos , Suspensiones
3.
Artículo en Ruso | MEDLINE | ID: mdl-19338232

RESUMEN

New Russian virosomal split vaccine against influenza "Grifor" was developed. The vaccine is represented by mix of highly purified protective external and internal antigens of influenza A (H1N1 and H3N2) and B viruses. Developed technology of manufacture allowed to provide presentation of external antigens of influenza virus in the form of virosomes, and presentation of internal antigens in the form of micelles with maximal preservation of their antigenic activity. Using electron microscopy, electrophoresis in 10% polyacrilamide gel with sodium dodecyl sulfate, and polymerase chain reaction, morphologic and biochemical properties of the vaccine were studied. Preclinical study, including assessment of antigenic characteristics of "Grifor" vaccine compared to vaccine "Vaxigrip" (France), was performed. It was established that administration of the vaccine did not result in death of experimental animals, decrease of body mass, development of pathologic (including inflammatory, dystrophic and necrobiotic) changes in viscera or render adverse effects on blood hematologic and biochemical parameters and on the immune system. The vaccine was not pyrogenic and allergenic, did not have local irritating effects. Obtained results supported the appropriateness of conducting the clinical trials of "Grifor" vaccine on limited number of volunteers.


Asunto(s)
Subtipo H1N1 del Virus de la Influenza A/inmunología , Subtipo H3N2 del Virus de la Influenza A/inmunología , Virus de la Influenza B/inmunología , Vacunas contra la Influenza/efectos adversos , Gripe Humana/prevención & control , Animales , Evaluación Preclínica de Medicamentos , Cobayas , Humanos , Hipersensibilidad/etiología , Esquemas de Inmunización , Vacunas contra la Influenza/administración & dosificación , Vacunas contra la Influenza/inmunología , Inyecciones Intraperitoneales , Ratones , Ratones Endogámicos BALB C , Micelas , Conejos , Vacunación , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/efectos adversos , Vacunas Sintéticas/inmunología , Virosomas/administración & dosificación
4.
Artículo en Ruso | MEDLINE | ID: mdl-18038551

RESUMEN

Real-time multiplex polymerase chain reaction (PCR) with internal positive control (IPC) was developed for simultaneous detection of adenoviruses (AV), enteroviruses (EV) and hepatitis A virus (HAV). Primes and probes labeled with different fluorophores (FAM, R6G, ROX, and Cy5) and able to detect up to four viral RNAs (DNAs) with high specificity in a single tube in real-time PCR were designed. Sensitivity and specificity of the method was estimated using cultural strains of 8 serotypes of EV, 5 serotypes of AV and 2 clinical specimens containing HAV. Sensitivity of the method for detection of polioviruses types 1, 2, and 3 (Sabin vaccine strains) was 0.5--1 TCID50 per reaction mixture. Thirty clinical specimens were analyzed by the multiplex PCR with and without IPC, and by mono-specific PCR. Comparison of these methods with cultural one revealed results agreement in 86.7% in case of multiplex PCR with IPC and in 100% in case of multiplex PCR without IPC and mono-specific PCR. This method can be used for rapid diagnostics of enteric viral infections as well as for determination of viral contamination level of water. As intermediate results of the study the methods for quantitative assessment of HAV, AV, and EV nucleic acids were developed which are convenient tools for the control of antiviral therapy effectiveness.


Asunto(s)
Infecciones por Adenoviridae/diagnóstico , Adenoviridae/aislamiento & purificación , Infecciones por Enterovirus/diagnóstico , Enterovirus/aislamiento & purificación , Virus de la Hepatitis A/aislamiento & purificación , Hepatitis A/diagnóstico , Reacción en Cadena de la Polimerasa/métodos , Adenoviridae/genética , Línea Celular , Cartilla de ADN , ADN Viral/genética , Enterovirus/genética , Virus de la Hepatitis A/genética , Humanos , ARN Viral/genética , Sensibilidad y Especificidad , Microbiología del Agua
5.
Mol Gen Mikrobiol Virusol ; (12): 42-7, 1988 Dec.
Artículo en Ruso | MEDLINE | ID: mdl-2855092

RESUMEN

Virosomes were prepared by using the zwitterion detergent sulfobetaine-12. The virosomes included the surface antigens and virus-specific lipids of influenza virus, strain A/PR/8/34. Immunogenic and protective properties of the surface antigens in the micellar form and as a complex with the virosomes were studied. The surface antigens of this complex, like the intact virus, were found to possess the high immunogenic and protective activity in relation to the following infection with the homologous pathogenic virus.


Asunto(s)
Detergentes , Virus de la Influenza A/efectos de los fármacos , Compuestos de Amonio Cuaternario , Tensoactivos , Animales , Anticuerpos/análisis , Virus de la Influenza A/inmunología , Ratones , Infecciones por Orthomyxoviridae/inmunología , Infecciones por Orthomyxoviridae/prevención & control , Solubilidad
6.
Vopr Virusol ; (6): 655-9, 1975.
Artículo en Ruso | MEDLINE | ID: mdl-1226702

RESUMEN

A culture of transformed cells, T-9 line, producing LPV oncornavirus and a culture of human diploid cells (HDC) infected with a cell-free ultrafiltrate of these transformed cells were examined in the electron microscope. The study was aimed at determination of the optimal time and intensity of LPV oncornavirus production by one cell of transformed and infected cultures. LPV oncornavirus production in T-9 cell culture was found to have a pattern of slowly developing, non-intensive two-wave process, whereas in infected HDC culture the process was progressing actively resulting in an increase of the amount of intracytoplasmic type A particles and extracellular type B and C virions by 10- and 15-fold, respectively, within 48- 96 hours for T-9 culture and 96 hours for infected HDC. Cell transformation was found to be apparently preceeded by the infectious process the intensity of which decreased as fibroblast cell culture was transformed into a culture of epithelioid cells.


Asunto(s)
Virus Oncogénicos/crecimiento & desarrollo , Replicación Viral , Línea Celular
7.
Vopr Virusol ; (6): 660-6, 1975.
Artículo en Ruso | MEDLINE | ID: mdl-1226703

RESUMEN

The results of immunological study of oncornavirus (LPV strain) produced by a continuous human T-9 cell line are presented. The method of immunodiffusion in agar revealed the following constituent antigens in the virus: (a) those identical to internal and external antigens of Mason-Pfizer oncornaviruses particles; (b) those specific only for LPV virus and not common in other known oncornaviruses of mammals. These antigens were common in LPV virus from T-9 culture and from xenogeneic T-BHK culture in which Mason-Pfizer virus antigens were absent completely; and (c) interspecific antigen of oncornavirus C type present in minimal amounts. Localization of the above antigens in virus particles of LPV oncornavirus was confirmed by the electron immunoenzyme method. Upon separation of virus polypeptides in polyacrylamide gel it was found that the antigens cross-reacting with Mason-Pfizer virus as well as LPV specific antigens were localized both in the main inner virus protein and in the surface glycoprotein.


Asunto(s)
Antígenos Virales/análisis , Virus Oncogénicos/inmunología , Línea Celular
8.
Vopr Virusol ; 33(2): 206-11, 1988.
Artículo en Ruso | MEDLINE | ID: mdl-3414068

RESUMEN

Electron microscopic examination of isolated intracellular measles virus nucleocapsids (NC) revealed a relationship between their structure, cell system, and the type of infection. Acute virus infection of Vero or Japanese quail embryo cells gave rise to the formation of linear NC strands with regularly and tightly stacked turns. Acutely infected L-41 or HEp-2 cells contained heteromorphous viral NC populations which included both typical and loosely packed NC. Persistently infected L-41 and Hep-2 cells predominantly contained NC of the latter type with the appearance of a "strings of beads".


Asunto(s)
Cápside/análisis , Virus del Sarampión/ultraestructura , Sarampión/microbiología , Proteínas del Núcleo Viral/análisis , Animales , Cápside/aislamiento & purificación , Virus del Sarampión/aislamiento & purificación , Microscopía Electrónica , Proteínas del Núcleo Viral/aislamiento & purificación , Cultivo de Virus
9.
Vopr Virusol ; 31(5): 567-72, 1986.
Artículo en Ruso | MEDLINE | ID: mdl-2432728

RESUMEN

The properties of virus-specific RNPs recovered from human HEp-2 and L-41 cells chronically infected with measles virus were studied in comparison with those of RNPs formed in acute infection of L-41 cells. The persisting RNP was shown to contain nucleoprotein not differing in the electrophoretic mobility from the same protein of measles virus virions, and RNA in the persisting RNP was found to be insensitive to the action of RN-ase. RNP from chronically infected cells had a changed ultrastructure and conformation as compared with RNP of the original virus and, unlike the latter had no infectivity upon transfection of the sensitive cells by calcium-phosphate precipitation. No differences in relationships of RNP with the cytoskeleton of the infected cells in the acute and chronic infection were observed.


Asunto(s)
Virus del Sarampión/patogenicidad , Ribonucleoproteínas/análisis , Proteínas Virales/análisis , Antígenos Virales/análisis , Cápside/análisis , Cápside/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Epítopos/análisis , Técnica del Anticuerpo Fluorescente , Humanos , Sarampión/microbiología , Virus del Sarampión/análisis , Virus del Sarampión/genética , Microscopía Electrónica , Ribonucleoproteínas/aislamiento & purificación , Transfección , Proteínas del Núcleo Viral/análisis , Proteínas del Núcleo Viral/aislamiento & purificación , Proteínas Virales/aislamiento & purificación , Cultivo de Virus
10.
Vopr Virusol ; 37(4): 199-204, 1992.
Artículo en Ruso | MEDLINE | ID: mdl-1281945

RESUMEN

The authors prepared 156 mouse hybridomas producing monoclonal (MCA) antibodies to type- and group-specific antigenic determinants of HSV-1 and HSV-2. Seven of them were studied at length by western blot and radioimmunoprecipitation methods. The cell lines 1H97 and 2H141 were shown to produce immunoglobulins of G2 beta and M class, respectively, and were directed against group-specific antigenic determinants of the major nucleocapsid protein p150. The cell lines 1H38 and 1H110 produced immunoglobulins of M and G2 beta, respectively, and were directed against type-specific antigens of HSV-1 glycoprotein gB. At the same time, the presence of group-specific antigenic determinants on glycoprotein gB molecule was indicated by MCA 1H188 belonging to immunoglobulins of G2 alpha class. Two cell lines, 2H208 and 1H225, produced immunoglobulins G2 alpha directed against type-specific antigenic determinants of HSV-2 glycoprotein gD and group-specific antigenic determinants of HSV-1 gD, respectively. The results of immunoelectron microscopy indicated that MCA 1H110 and 2H208 were directed against virus envelope proteins.


Asunto(s)
Anticuerpos Monoclonales/aislamiento & purificación , Simplexvirus/inmunología , Proteínas Virales/inmunología , Animales , Anticuerpos Monoclonales/análisis , Línea Celular , Núcleo Celular/inmunología , Reacciones Cruzadas , Citoplasma/inmunología , Epítopos/inmunología , Epítopos/aislamiento & purificación , Femenino , Hibridomas/inmunología , Inmunización , Immunoblotting , Ratones , Ratones Endogámicos BALB C , Microscopía Inmunoelectrónica , Simplexvirus/aislamiento & purificación , Proteínas Virales/aislamiento & purificación
11.
Vopr Virusol ; (4): 445-9, 1976.
Artículo en Ruso | MEDLINE | ID: mdl-188252

RESUMEN

Electron microscopy and mathematical analysis were used to determine the intensity of LPV oncornavirus production by a single cell in co-cultivated human diploid cells and cells of the continuous T-9 line. Maximum production of intracytoplasmic particles of A type was observed at 48 hours of cultivation, and extracellular virions at 96 hours. Mature virions of B type were more numerous than mature virions of C type in the mixed culture. On the whole the amolnt of mature virions was greater than that of immature ones, and the amount of intracytoplasmic A type particles was considerably greater than that of extracellular particles. By the total amount of production of all virus-specific structures and the two-wave pattern of virus production this mixed culture passaged at a ratio of HDC to T-9 cells 2000 : 1 resembled a culture of T-9 line. Thus, this study indicates that the infected HDC culture at later intervals of cultivation (96 hours) is a more active "producer" of LPV oncornavirus than the main line of T-9 cells or mixed HDC--T-9 culture.


Asunto(s)
Virus Oncogénicos/crecimiento & desarrollo , Replicación Viral , Línea Celular , Citoplasma/microbiología , Humanos , Células Híbridas , Virus Oncogénicos/aislamiento & purificación , Retroviridae/aislamiento & purificación
12.
Vopr Virusol ; (4): 486-90, 1976.
Artículo en Ruso | MEDLINE | ID: mdl-64025

RESUMEN

Some properties of 4 diplod cell strains derived from the lung and skin-muscle tissue of human embryos were studied. Only one strain (L-63) was shown to have a stable karyotype throughout its life span and induced no tumor formation in the cheek pouch of hamsters. Species-specific and group antigens were revealed. In the cell strains of the lung and skin-muscle derivation differences in the distribution of lactate dehydrogenase isoenzymes were observed and found characteristic of the original tissue cells.


Asunto(s)
Línea Celular , Animales , Cricetinae , Diploidia , Epítopos , Humanos , Isoenzimas , L-Lactato Deshidrogenasa/metabolismo , Pulmón/embriología , Músculos/embriología , Neoplasias Experimentales/etiología , Piel/embriología
13.
Vopr Virusol ; 27(4): 456-61, 1982.
Artículo en Ruso | MEDLINE | ID: mdl-6182696

RESUMEN

The results of a comparative study of the sensitivity of several methods for mycoplasma detection in cell cultures are presented. The most sensitive method was found to be that of electron microscopy detecting mycoplasmal contamination in 100% preparations. Seeding of the material on mycoplasma-elective nutrient medium (0.3% PPLO agar) and the method of autoradiography detect the culture contamination in 90% of the test materials. Staining of cell cultures with orsein and Schiff reagent are less sensitive methods revealing mycoplasmal contamination in 69% and 77.7% of the cultures, respectively.


Asunto(s)
Mycoplasma/aislamiento & purificación , Autorradiografía , Técnicas Bacteriológicas , Células Cultivadas , Centrifugación por Gradiente de Densidad , Medios de Cultivo/farmacología , Microscopía Electrónica , Fenilendiaminas/farmacología , Coloración y Etiquetado/métodos , Compuestos de Sulfhidrilo/farmacología
14.
Vopr Virusol ; 27(5): 22-7, 1982.
Artículo en Ruso | MEDLINE | ID: mdl-7147898

RESUMEN

Viral nucleocapsids isolated from cell cultures of human origin (HEp-2 and L-41) persistently infected with mumps virus for 4 years were analysed. In persistent infection, nucleocapsids accumulated in the cells in greater amounts than in primary infection. The synthesis of NP protein of nucleocapsids in persistent infection was approximately 10 times lower than that in primary infection. Morphologically, nucleocapsids in primary infection are rigid linear helical structures whereas in persistent infection they are of convoluted shape, fragmented, and partially or completely despiralized and destructuralized.


Asunto(s)
Cápside/biosíntesis , Virus de la Parotiditis/metabolismo , Nucleoproteínas/biosíntesis , Ribonucleoproteínas/biosíntesis , Proteínas Virales/biosíntesis , Línea Celular , Conformación Proteica , Factores de Tiempo
15.
Vopr Virusol ; (2): 153-7, 1980.
Artículo en Ruso | MEDLINE | ID: mdl-7385786

RESUMEN

Influenza virus nucleoid ("core") was obtained by the treatment of virions with hydrolytic enzymes without the use of fixing agents. The "core" buoyant density in sucrose density gradient was 1.24 g/cm3. The subvirus particles are completely devoid of surface proteins, glycoproteins, and their polypeptide composition is represented by group P proteins; NP and M proteins. RNA in the core is insensitive to the effect of RNase. Morphological analysis by electron microscopy revealed the presence of spherical compact particles without spikes on their surface.


Asunto(s)
Virus de la Influenza A/ultraestructura , Animales , Embrión de Pollo , Quimotripsina/farmacología , Glicoproteínas/análisis , Virus de la Influenza A/análisis , Virus de la Influenza A/efectos de los fármacos , Virus de la Influenza A/aislamiento & purificación , Péptidos/análisis , Péptidos/clasificación , Fosfolipasas/farmacología , ARN Viral/metabolismo , Ribonucleasas/farmacología , Proteínas Virales/análisis , Proteínas Virales/clasificación , Virión/ultraestructura
16.
Vopr Virusol ; (5): 560-4, 1978.
Artículo en Ruso | MEDLINE | ID: mdl-735016

RESUMEN

An agent isolated from Japanese quail embryos was found to be pathogenic for chick embryo and quail embryo fibroblast cultures, to cause hemadsorption and hemagglutination of guinea pig erythrocytes. Reproduction of the agent was inhibited by tetracycline and 5-bromodeoxyuridine. The agent incorporated both 3H-thymidine and 3H-uridine and had a buoyant density in sucrose gradient of 1.22 g/ml. Mature mycoplasma forms were determined electron microscopically in gradient fractions in cell sections.


Asunto(s)
Coturnix/microbiología , Mycoplasma/aislamiento & purificación , Animales , Bromodesoxiuridina/farmacología , Línea Celular , Centrifugación por Gradiente de Densidad , Coturnix/embriología , Éter/farmacología , Mycoplasma/efectos de los fármacos , Mycoplasma/crecimiento & desarrollo , Tetraciclina/farmacología
17.
Vopr Virusol ; (2): 224-8, 1983.
Artículo en Ruso | MEDLINE | ID: mdl-6868562

RESUMEN

Purified virions of the vaccine strain Leningrad-3 of mumps virus propagated in Japanese quail embryo cell cultures had a buoyant density 1.18-1.19 g/ml in sucrose gradient, contained 50 S RNA and showed variable sizes in electron microscopy as manifested by heterogeneity of the virus zone in sedimentation analysis. Purified L-3 virus contained 5 major polypeptides with molecular weights of 74,000, 68,000, 58,000, 45,000, and 39,000 daltons. Each polypeptide had an individual oligopeptide composition.


Asunto(s)
Vacuna contra la Parotiditis/análisis , Virus de la Parotiditis/análisis , Fenómenos Químicos , Química Física , Electroforesis en Gel de Poliacrilamida , Microscopía Electrónica , Peso Molecular , Virus de la Parotiditis/aislamiento & purificación , Virus de la Parotiditis/ultraestructura , Oligopéptidos/análisis , Péptidos/análisis , Virión/análisis , Virión/ultraestructura
18.
Vopr Virusol ; 35(1): 64-8, 1990.
Artículo en Ruso | MEDLINE | ID: mdl-2363277

RESUMEN

The properties of lymphoid Namalwa cell line propagated at the USSR Academy of Medical Sciences Research Institute of Viral Preparations for interferon production are described. The scanning and transmissive electron microscopy studies of the cells showed their morphological stability and the absence of microbial contamination. The 46-48-chromosome cells comprised 85% of the population, hypodiploid cells (44-45 chromosomes), 9%, tetraploid and hypertetraploid cells, 3%. Spontaneous aberrations were detected in 3% of the chromosome. Inoculation of the cells into unsuppressed laboratory animals (rabbits, guinea pigs, adult or suckling mice) or chick embryos did not cause the development of any pathological process. Namalwa cells were shown to produce interferon after multiple (up to 4 times) induction with Newcastle disease virus.


Asunto(s)
Línea Celular , Interferón Tipo I/biosíntesis , Linfoma de Burkitt , Línea Celular/citología , Línea Celular/metabolismo , Línea Celular/ultraestructura , Humanos , Inductores de Interferón , Cariotipificación , Microscopía Electrónica de Rastreo
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