Development and On-Field Deployment of a Mobile-Based Application 'MoSQuIT' for Malaria Surveillance in International Border Districts of Northeast India-Challenges and Opportunities.

The conventional paper-based system for malaria surveillance is time-consuming, difficult to track and resource-intensive. Few digital platforms are in use but wide-scale deployment and acceptability remain to be seen. To address this issue, we created a malaria surveillance mobile app that offers real-time data to stakeholders and establishes a centralised data repository. The MoSQuIT app was designed to collect data from the field and was integrated with a web-based platform for data integration and analysis. The MoSQuIT app was deployed on mobile phones of accredited social health activists (ASHA) working in international border villages in the northeast (NE) Indian states of Assam, Tripura and Arunachal Pradesh for 20 months in a phased manner. This paper shares the challenges and opportunities associated with the use of MoSQuIT for malaria surveillance. MoSQuIT employs the same data entry formats as the NVBDCP's malaria surveillance programme. Using this app, a total of 8221 fever cases were recorded, which included 1192 (14.5%) cases of P. falciparum malaria, 280 (3.4%) cases of P. vivax malaria and 52 (0.6%) mixed infection cases. Depending on network availability, GPS coordinates of the fever cases were acquired by the app. The present study demonstrated that mobile-phone-based malaria surveillance facilitates the quick transmission of data from the field to decision makers. Geospatial tagging of cases helped with easy visualisation of the case distribution for the identification of malaria-prone areas and potential outbreaks, especially in hilly and remote regions of Northeast India. However, to achieve the full operational potential of the system, operational challenges have to be overcome.

On the action and mechanism of withaferin-A from Withania somnifera, a novel and potent melanin dispersing agent in frog melanophores.

The present work was carried out to determine the effects of lyophilized root extracts of Withania somnifera along with pure withaferin-A, on the isolated skin melanophores of frog, Rana tigerina which are disguised type of smooth muscle cells and offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. The lyophilized extract of W. somnifera and its active ingredient withaferin-A induced powerful dose-dependent physiologically significant melanin dispersal effects in the isolated skin melanophores of R. tigerina, which were completely blocked by atropine as well as hyoscine. The per se melanin dispersal effects of lyophilized extracts of W. somnifera and its active ingredient withaferin-A got highly potentiated by neostigmine. It appears that the melanin dispersal effects of the extracts of W. somnifera and withaferin-A is mediated by cholino-muscarinic like receptors having similar properties.

In vitro responses of fish melanophores to lyophilized extracts of Psoralea corylifolia seeds and pure psoralen.

CONTEXT: Psoralens are naturally occurring furanocumarins used in photochemotherapy of several skin diseases. They are obtained from dried ripe fruits of Psoralea corylifolia Linn. (Fabaceae). However, little research has been done to study the melanogenic activity of P. corylifolia seeds and their active ingredients on the pigment cells, the melanophores taking account of their cholinergic activity. OBJECTIVE: The present work was carried out to determine the effects of lyophilized seed extracts of P. corylifolia, along with pure psoralen on the isolated scale melanophores of Channa punctatus Bloch. (Channidae), which are a disguised type of smooth muscle cells and offer excellent in vitro opportunities for studying the effects of drugs. MATERIALS AND METHODS: Effects of lyophilized extracts of P. corylifolia and pure psoralen were studied on the isolated scale melanophores of C. punctatus as per the modified method of Bhattacharya et al. (1976) . RESULTS: The lyophilized extract of P. corylifolia and its active ingredient psoralen caused significant melanin dispersal responses leading to darkening of the fish scale melanophores, which were completely antagonized by atropine and hyoscine. These melanin dispersal effects were also found to be markedly potentiated by neostigmine, an anticholinesterase agent. DISCUSSION: In the present study, the lyophilized extract of P. corylifolia seeds and standard psoralen in different dose ranges induced powerful melanin dispersal effects of the previously adrenaline-aggregated isolated scale melanophores of C. punctatus. Comparatively, psoralen caused a more sustained and powerful melanin dispersal within the isolated fish melanophores and interestingly the concentrations required to achieve maximal dispersion of melanophore were 10 times less than that of lyophilized seed extract of P. corylifolia. The physiologically significant dose-related melanin dispersion effects of lyophilized P. corylifolia seeds and synthetic psoralen per se were found to be completely abolished by atropine and hyoscine, which are specific cholino-muscarinic receptor blockers. These data strongly indicate that in the fish C. punctatus, the dispersion of melanin granules within the scale melanophores is mediated by choline receptors of muscarinic nature. CONCLUSION: It appears that the melanin dispersal effects of the extracts of P. corylifolia and pure psoralen leading to skin darkening are mediated by cholino-muscarinic- or cholino-psoralen-like receptors having similar properties.

Withania somnifera root extracts induce skin darkening in wall lizard melanophores via stimulation of cholinergic receptors.

The effects of the root extract of Withania somnifera and its active ingredient Withaferin A on the isolated melanophores of the wall lizard H. flaviviridis were studied in order to establish the mechanism of skin darkening at the cellular level. Significant skin darkening activity of the extract of W. somnifera and Withaferin A was observed on the isolated melanophores of the wall lizard. The melanin dispersal effects leading to the darkening of the skin were antagonised by atropine and hyoscine, and were also found to be highly potentiated by neostigmine. These findings suggest that the extract of W. somnifera, as well as its active principle, mimic the action of acetylcholine in melanin dispersion, thus leading to skin darkening via stimulation of cholinergic receptors of muscarinic nature within the melanophores of the wall lizard.

Nigella sativa seed extract and its bioactive compound thymoquinone: the new melanogens causing hyperpigmentation in the wall lizard melanophores.

OBJECTIVE: The effects of the lyophilized seed extract of Nigella sativa and its active ingredient, thymoquinone, were studied on the isolated melanophores of the wall lizard to find the mechanism of skin darkening at the cellular level. METHODS: The integumental melanophores of the wall lizard, Hemidactylus flaviviridis, were assayed using the mean melanophore size index and their responses were recorded in the presence of various concentrations of the plant extract, thymoquinone, specific antagonists and potentiator. KEY FINDINGS: Significant skin darkening activity of the extract of N. sativa and thymoquinone was observed on the isolated melanophores of the wall lizard. The pigment cells responded by distinct dispersion leading to skin darkening. The effect was physiologically significant as re-immersion in physiological saline made the melanophores return to their normal intermediate state. These melanin dispersal effects were antagonized by atropine as well as hyoscine and were also found to be highly potentiated by neostigmine, an anticholinesterase agent. CONCLUSIONS: These findings suggest that the extract of N. sativa, as well as its active principle, mimic the action of acetylcholine in melanin dispersion leading to skin darkening via stimulation of cholinergic receptors of muscarinic nature within the melanophores of wall lizard. This study opens new vistas for the use of N. sativa active ingredient, thymoquinone, as a novel melanogen for its clinical application in skin disorders such as hypopigmentation or vitiligo.