Chlorpyrifos enrichment enhances tolerance of Anabaena sp. PCC 7119 to dimethoate.

Organophosphorus (OP) insecticides are widely used for on-field pest control, constituting about 38% of global pesticide consumption. Insecticide tolerance has been recorded in microorganisms isolated from the contaminated soil. However, the cross-tolerance of laboratory-enriched cultures remains poorly understood. A chlorpyrifos tolerant (T) strain of Anabaena sp. PCC 7119 was developed through continuous enrichment of the wild strain (W). The cross-tolerance of the T strain to the OP insecticide dimethoate was assessed by measuring photosynthetic performance, key enzyme activities and degradation potential. The presence of dimethoate led to a significant reduction in the growth and pigment content of the W strain. In contrast, the T strain demonstrated improved growth and metabolic performance. Chl a and carotenoids were degraded faster than phycobiliproteins in both strains. The T strain exhibited superior photosynthetic performance, metabolic efficiency and photosystem functions, than of W strain, at both the tested dimethoate concentrations (100 and 200 µM). The treated T strain had more or less a normal OJIP fluorescence transient and bioenergetic functions, while the W strain showed a greater fluorescence rise at ≤ 300 µs indicating the inhibition of electron donation to PS II, and at 2 ms due to reduced electron release beyond QA. The T strain had significantly higher levels of esterase and phosphatases, further enhanced by insecticide treatment. Dimethoate degradation efficiency of the T strain was significantly higher than of the W strain. T strain also removed chlorpyrifos more efficiently than W strain at both the tested concentrations. The BCFs of both chlorpyrifos and dimethoate were lower in the T strain compared to the W strain. These findings suggest that the enriched strain exhibits promising results in withstanding dimethoate toxicity and could be explored for its potential as a bioremediating organism for OP degradation.

To decipher the phytochemical agent and mechanism for Urginea indica mediated green synthesis of Ag nanoparticles and investigation of its antibacterial activity against Methicillin-resistant Staphylococcus aureus.

Globally, Methicillin-Resistant Staphylococcus aureus bacteraemia is one of the commonest bloodstream infections associated with clinical complications and high mortality. Thence, devising effective and targeted biogenic silver based strategies are in great demand. However, limited insights regarding the biosynthesis methodologies impedes the possible scale up and commercial potentials. We, hereby demonstrate the biosynthesis of Ag nanoparticles using the phytochemical agent extracted and purified from bulb extract of Urginea indica. The chemical structure of the phytochemical agent is investigated by various chromatographic and spectroscopic techniques and was found closely relatable to N-ethylacetamide. Ag nanoparticles synthesis by this agent was found to have a strong Surface Plasmon band at 402 nm. X-ray diffraction and transmission electron microscopy further validated the formation of Ag nanoparticles with face-centred cubic structure with a size range of 20-30 nm. The biogenic metal nanoparticles have shown potential antibacterial activity against S. aureus and MRSA (within a range of 10-50 µg/mL). The nanoparticles have also shown promising anti-biofim activity against the above mentioned strains. The nanoparticles were expected to induce ROS mediated bactericidal mechamism. Cell viability and in-vitro infection studies advocate noticeable biocompatibility and future clinical potential of the developed nanoparticles against Staphylococcus infections.

Carbon Dots and Their Polymeric Nanocomposites: Insight into Their Synthesis, Photoluminescence Mechanisms, and Recent Trends in Sensing Applications.

Carbon dots (CDs), a novel class of carbon-based nanoparticles, have received a lot of interest recently due to their exceptional mechanical, chemical, and fluorescent properties, as well as their excellent photostability and biocompatibility. CDs' emission properties have already found a variety of potential applications, in which bioimaging and sensing are major highlights. It is widely acknowledged that CDs' fluorescence and surface conditions are closely linked. However, due to the structural complexity of CDs, the specific underlying process of their fluorescence is uncertain and yet to be explained. Because of their low toxicity, robust and wide optical absorption, high chemical stability, rapid transfer characteristics, and ease of modification, CDs have been recognized as promising carbon nanomaterials for a variety of sensing applications. Thus, following such outstanding properties of CDs, they have been mixed and imprinted onto different polymeric components to achieve a highly efficient nanocomposite with improved functional groups and properties. Here, in this review, various approaches and techniques for the preparation of polymer/CDs nanocomposites have been elaborated along with the individual characteristics of CDs. CDs/polymer nanocomposites recently have been highly demanded for sensor applications. The insights from this review are detailed sensor applications of polymer/CDs nanocomposites especially for detection of different chemical and biological analytes such as metal ions, small organic molecules, and several contaminants.

Chemopriming for induction of disease resistance against pathogens in rice.

Rice is an important grain crop of Asian population. Different fungal, bacterial and viral pathogens cause large reduction in rice grain production. Use of chemical pesticides, to provide protection against pathogens, has become incomplete due to pathogens resistance and is cause of environmental concerns. Therefore, induction of resistance in rice against pathogens via biopriming and chemopriming with safe and novel agents has emerged on a global level as ecofriendly alternatives that provide protection against broad spectrum of rice pathogens without any significant yield penalty. In the past three decades, a number of chemicals such as silicon, salicylic acid, vitamins, plant extract, phytohormones, nutrients etc. have been used to induce defense against bacterial, fungal and viral rice pathogens. From the detailed analysis of abiotic agents used, it has been observed that silicon and salicylic acid are two potential chemicals for inducing resistance against fungal and bacterial diseases in rice, respectively. However, an inclusive evaluation of the potential of different abiotic agents to induce resistance against rice pathogens is lacking due to which the studies on induction of defense against rice pathogens via chemopriming has become disproportionate and discontinuous. The present review deals with a comprehensive analysis of different abiotic agents used to induce defense against rice pathogens, their mode of application, mechanism of defense induction and the effect of defense induction on grain yield. It also provides an account of unexplored areas, which might be taken into attention to efficiently manage rice diseases. DATA AVAILABILITY STATEMENT: Data sharing not applicable to this article as no datasets were generated or analysed during the current study.