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1.
Mol Psychiatry ; 27(5): 2602-2618, 2022 05.
Artículo en Inglés | MEDLINE | ID: mdl-35246635

RESUMEN

A hallmark of the anterior cingulate cortex (ACC) is its functional heterogeneity. Functional and imaging studies revealed its importance in the encoding of anxiety-related and social stimuli, but it is unknown how microcircuits within the ACC encode these distinct stimuli. One type of inhibitory interneuron, which is positive for vasoactive intestinal peptide (VIP), is known to modulate the activity of pyramidal cells in local microcircuits, but it is unknown whether VIP cells in the ACC (VIPACC) are engaged by particular contexts or stimuli. Additionally, recent studies demonstrated that neuronal representations in other cortical areas can change over time at the level of the individual neuron. However, it is not known whether stimulus representations in the ACC remain stable over time. Using in vivo Ca2+ imaging and miniscopes in freely behaving mice to monitor neuronal activity with cellular resolution, we identified individual VIPACC that preferentially activated to distinct stimuli across diverse tasks. Importantly, although the population-level activity of the VIPACC remained stable across trials, the stimulus-selectivity of individual interneurons changed rapidly. These findings demonstrate marked functional heterogeneity and instability within interneuron populations in the ACC. This work contributes to our understanding of how the cortex encodes information across diverse contexts and provides insight into the complexity of neural processes involved in anxiety and social behavior.


Asunto(s)
Giro del Cíngulo , Péptido Intestinal Vasoactivo , Animales , Giro del Cíngulo/metabolismo , Interneuronas/metabolismo , Ratones , Neuronas/metabolismo , Células Piramidales/metabolismo , Péptido Intestinal Vasoactivo/metabolismo
2.
PLoS Biol ; 18(1): e3000604, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31935214

RESUMEN

Schizophrenia is a severe mental disorder with an unclear pathophysiology. Increased expression of the immune gene C4 has been linked to a greater risk of developing schizophrenia; however, it is not known whether C4 plays a causative role in this brain disorder. Using confocal imaging and whole-cell electrophysiology, we demonstrate that overexpression of C4 in mouse prefrontal cortex neurons leads to perturbations in dendritic spine development and hypoconnectivity, which mirror neuropathologies found in schizophrenia patients. We find evidence that microglia-mediated synaptic engulfment is enhanced with increased expression of C4. We also show that C4-dependent circuit dysfunction in the frontal cortex leads to decreased social interactions in juvenile and adult mice. These results demonstrate that increased expression of the schizophrenia-associated gene C4 causes aberrant circuit wiring in the developing prefrontal cortex and leads to deficits in juvenile and adult social behavior, suggesting that altered C4 expression contributes directly to schizophrenia pathogenesis.


Asunto(s)
Complemento C4/genética , Neuronas/fisiología , Corteza Prefrontal/citología , Esquizofrenia/genética , Conducta Social , Envejecimiento/genética , Envejecimiento/metabolismo , Envejecimiento/patología , Animales , Animales Recién Nacidos , Comunicación Celular/genética , Células Cultivadas , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Vías Nerviosas/metabolismo , Corteza Prefrontal/patología , Esquizofrenia/patología , Regulación hacia Arriba/genética
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